Extraction of genomic DNA from paraffin-embedded tissue sections of human fetuses fixed and stored in formalin for long periods

Pathology Research and Practice

Volumn 204, Issue 9, 2008, Pages 633-636

  Santos, Maria C L G ^a   Saito, Cristiane P B ^a   Line, Sergio R P ^a  

^a UNIVERSITY OF CAMPINAS   (Brazil)

Author keywords

DNA extraction; Formalin fixed; Microdissection; Museum specimens; Paraffin embedded

Indexed keywords

ALBUMIN; ALCOHOL; AMMONIUM ACETATE; DNA FRAGMENT; FORMALDEHYDE; GENOMIC DNA; PARAFFIN; XYLENE;

ARTICLE; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; DNA EXTRACTION; FETUS; GENE AMPLIFICATION; HUMAN; HUMAN TISSUE; INFANT; MICRODISSECTION; POLYMERASE CHAIN REACTION; QUALITATIVE ANALYSIS; RELIABILITY; TIME; TISSUE PRESERVATION; UMBILICAL CORD;

DNA; FETUS; FORMALDEHYDE; HUMANS; MICRODISSECTION; PARAFFIN EMBEDDING; POLYMERASE CHAIN REACTION; TISSUE FIXATION;

EID: 49749125216     PISSN: 03440338     EISSN: 16180631     Source Type: Journal    
DOI: 10.1016/j.prp.2008.04.005     Document Type: Article

References (24)

1. Barnes I., Holton J., Vaira D., Spigelman M., and Thomas M.G. An assessment of the long-term preservation of the DNA of a bacterial pathogens in ethanol-preserved archival material. J. Pathol. 192 (2000) 554-559
2. Becker I., Becker K.F., Rohrl M.H., Minkus G., Schutze K., and Hofler H. Single-cell mutation analysis of tumors from stained histologic slides. Lab. Invest. 75 (1996) 801-807
3. Ben-Ezra J., Johnson D.A., Rossi J., Cook N., and Wu A. Effect of fixation on the amplification of nucleic acids from paraffin embedded material by the polymerase chain reaction. J. Histochem. Cytochem. 39 (1991) 351-354
4. Cao W., Hashibe M., Rao J.-Y., Morgenstern H., and Zhang Z.F. Comparison of methods for DNA extraction from paraffin-embedded tissues and buccal cells. Cancer Detect. Prev. 27 (2003) 397-404
5. Coura R., Prolla J.C., Meurer L., and Ashton-Prolla P. An alternative protocol for DNA extraction from formalin fixed and paraffin wax embedded tissue. J. Clin. Pathol. 58 (2005) 894-895
6. Elliott K., Hill D.S., Lambert C., Burroughes T.R., and Gill P. Use of laser microdissection greatly improves the recovery of DNA from sperm on microscope slides. Forensic Sci. Int. 137 (2003) 28-36
7. Feigelson H.S., Rodriguez C., Robertson A.S., Jacobs E.J., Calle E.E., Reid Y.A., and Thun M.J. Determinants of DNA yield and quality from buccal cell samples collected with mouthwash. Cancer Epidemiol. Biomarkers Prev. 9 (2001) 1005-1008
8. Garcia-Closas M., Egan K.M., Abruzzo J., Newcomb P.A., Titus-Ernstoff L., Franklin T., Bender P.K., Beck J.C., Le Marchand L., Lum A., Alavanja M., Hayes R.B., Rutter J., Buetow K., Brinton L.A., and Rothman N. Collection of genomic DNA from adults in epidemiological studies by buccal cytobrush and mouthwash. Cancer Epidemiol. Biomarkers Prev. 6 (2001) 687-696
9. Genest D.R., Granter S., and Pinkus G.S. Umbilical cord 'pseudo-vasculitis' following second trimester fetal death: a clinicopathological and immunohistochemical study of 13 cases. Histopathology 30 (1997) 563-569
10. Gloghini A., Canal B., Klein U., Dal Maso L., Perin T., Dalla-Favera R., and Carbone A. RT-PCR analysis of RNA extracted from Bouin-fixed and paraffin-embedded lymphoid tissues. J. Mol. Diagn. 6 (2004) 290-296
11. Huang Q., Sacks P.G., Mo J., McCormick S.A., Iacob C.E., Guo L., Schaefer S., and Schantz S.P. A simple method for fixation and microdissection of frozen fresh tissue sections for molecular cytogenetic analysis of cancers. Biotech. Histochem. 80 (2005) 147-156
12. Ikuta N., Kimura E.A., Taque J., Lunge V.R., Da Silva L.P., Debes A., and Ozaki L.S. Non-phenolic method of DNA purification from bacteria, blood samples and other biological sources for restriction enzyme assays and the polymerase chain reaction. Mem. Inst. Oswaldo Cruz. 4 (1992) 589-591
13. King I.B., Satia-Abouta J., Thornquist M.D., Bigler J., Patterson R.E., Kristal A.R., Shattuck A.L., Potter J.D., and White E. Buccal cell DNA yield, quality, and collection costs: comparison of methods for large-scale studies. Cancer Epidemiol. Biomarkers Prev. 10 (2002) 1130-1133
14. Kitazawa S., Kitazawa R., and Maeda S. Identification of methylated cytosine from archival formalin-fixed paraffin-embedded specimens. Lab. Invest. 80 (2000) 275-276
15. Krishna T.G., and Jawali N. DNA isolation from single or half seeds suitable for random amplified polymorphic DNA analyses. Anal. Biochem. 250 (1997) 125-127
16. Rivero E.R.C., Neves A.C., Silva-Valenzuela M.G., Sousa S.O.M., and Nunes F.D. Simple salting-out method for DNA extraction from fromalin-fixed paraffin-embedded tissues. Pathol. Res. Pract. 202 (2006) 523-529
17. Robino C., Barilaro M.R., Gino S., Chiarle R., Palestro G., and Torre C. Incestuous paternity detected by STR-typing of chorionic villi isolated from archival formalin-fixed paraffin-embedded abortion material using laser microdissection. J. Forensic Sci. 51 (2006) 90-92
18. Rogers B.B., Alpert L.C., Hine E.A.S., and Buffone G.J. Analysis of DNA in fresh and fixed tissue by the polymerase chain reaction. Am. J. Pathol. 136 (1990) 541-548
19. Sanguinetti C.J., Dias Neto E., and Simpson A.J. Rapid silver staining and recovery of PCR products separated on polyacrylamide gels. Biotechniques 5 (1994) 914-921
20. Savioz A., Blouin J.L., Guidi S., Antonarakis S.E., and Bouras C. A method for the extraction of genomic DNA from human brain tissue fixed and stored in formalin for many years. Acta Neuropathol. 93 (1997) 408-413
21. Singh H.B., Katoch V.M., Natrajan M., Sharma V.D., Chauhan D.S., Lavania M., Sharma P., Sharma M., Katoch K., Benara S., and Singh P. Improved protocol for PCR detection of Mycobacterium leprae in buffered formalin-fixed skin biopsies. Int. J. Lepr. Mycobact. Dis. 72 (2004) 175-178
22. Tonnies H., Gerlach A., Klunker G., Schultka R., and Gobbel L. First systematic CGH-based analyses of ancient DNA samples of malformed fetuses preserved in the Meckel Anatomical Collection in Halle/Saale (Germany). J. Histochem. Cytochem. 53 (2005) 381-384
23. Vachot A.M., and Monnerot M. Extraction, amplification and sequencing of DNA from formaldehyde-fixed specimens. Ancient Biomol. 1 (1996) 3-16
24. Walker A.H., Najarian D., White D.L., Jaffe J.F., Kanetsky P.A., and Rebbeck T.R. Collection of genomic DNA by buccal swabs for polymerase chain reaction-based biomarker assays. Environ. Health Perspect. 7 (1999) 517-520