Monitoring immune cells trafficking fluorescent prion rods hours after intraperitoneal infection

Journal of Visualized Experiments

Volumn , Issue 45, 2010, Pages

  Johnson, Theodore E ^a   Michel, Brady A ^a   Meyerett, Crystal ^a   Duffy, Angela ^a   Avery, Anne ^a   Dow, Steven ^a   Zabel, Mark D ^a  

^a Department of Environmental and Radiological Health Sciences   (United States)

Author keywords

Flow cytometry; Immunology; Intraperitoneal; Issue 45; Lymph nodes; Mouse; Prions; Trafficking

Indexed keywords

EID: 80355145342     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2349     Document Type: Article

References (19)

1. Klein, M. A. et al., A crucial role for B cells in neuroinvasive scrapie. Nature 390 (6661), 687 (1997).
2. Mabbott, N. A., Farquhar, C. F., Brown, K. L., and Bruce, M. E., Involvement of the immune system in TSE pathogenesis. Immunol Today 19 (5), 201 (1998).
3. Sigurdson, C. J. et al., PrP(CWD) lymphoid cell targets in early and advanced chronic wasting disease of mule deer. J Gen Virol 83 (Pt 10), 2617 (2002).
4. Klein, M. A. et al., Complement facilitates early prion pathogenesis. Nat Med 7 (4), 488 (2001).
5. Mabbott, N. A. et al., Temporary depletion of complement component C3 or genetic deficiency of C1q significantly delays onset of scrapie. Nat Med 7 (4), 485 (2001).
6. Zabel, M. D. et al., Stromal Complement Receptor CD21/35 Facilitates Lymphoid Prion Colonization and Pathogenesis. J Immunol 179 (9), 6144 (2007).
7. Cordier-Dirikoc, S. and Chabry, J., Temporary depletion of CD11c+ dendritic cells delays lymphoinvasion after intraperitonal scrapie infection. J Virol 82 (17), 8933 (2008).
8. Dorban, G. et al., Oral scrapie infection modifies the homeostasis of Peyer's patches' dendritic cells. Histochem Cell Biol 128 (3), 243 (2007).
9. Flores-Langarica, A. et al., Scrapie pathogenesis: the role of complement C1q in scrapie agent uptake by conventional dendritic cells. J Immunol 182 (3), 1305 (2009).
10. Huang, F. P. and MacPherson, G. G., Dendritic cells and oral transmission of prion diseases. Adv Drug Deliv Rev 56 (6), 901 (2004).
11. Ano, Y., Sakudo, A., Nakayama, H., and Onodera, T., Uptake and dynamics of infectious prion protein in the intestine. Protein Pept Lett 16 (3), 247 (2009).
12. Aucouturier, P. et al., Infected splenic dendritic cells are sufficient for prion transmission to the CNS in mouse scrapie. J Clin Invest 108 (5), 703 (2001).
13. Huang, F. P. et al., Migrating intestinal dendritic cells transport PrP(Sc) from the gut. J Gen Virol 83 (Pt 1), 267 (2002).
14. Jeffrey, M. et al., Transportation of prion protein across the intestinal mucosa of scrapiesusceptible and scrapie-resistant sheep. J Pathol 209 (1), 4 (2006).
15. Raymond, C. R. and Mabbott, N. A., Assessing the involvement of migratory dendritic cells in the transfer of the scrapie agent from the immune to peripheral nervous systems. J Neuroimmunol 187 (1-2), 114 (2007).
16. Mohan, J., Hopkins, J., and Mabbott, N. A., Skin-derived dendritic cells acquire and degrade the scrapie agent following in vitro exposure. Immunology 116 (1), 122 (2005).
17. Gilch, S. et al., CpG and LPS can interfere negatively with prion clearance in macrophage and microglial cells. FEBS J 274 (22), 5834 (2007).
18. Safar, J. et al., Molecular mass, biochemical composition, and physicochemical behavior of the infectious form of the scrapie precursor protein monomer. Proc. Natl. Acad. Sci. U.S.A. 87, 6373 (1990).
19. Büeler, H.R. et al., Mice devoid of PrP are resistant to scrapie. Cell 73 (7), 1339 (1993).