Cellfood™ improves respiratory metabolism of endothelial cells and inhibits hypoxia-induced rective oxygen species (ROS) generation

Journal of Physiology and Pharmacology

Volumn 62, Issue 3, 2011, Pages 287-293

  Ferrero, E ^a   Fulgenzi, A ^b   Belloni, D ^a   Foglieni, C ^a   Ferrero, M E ^b  

^a SAN RAFFAELE HOSPITAL   (Italy)

^b UNIVERSITY OF MILAN   (Italy)

Author keywords

Cellfood ; Human umbilical vein endothelial cells; Hypoxia inducible factor 1alpha; Mitochondrial activity; MnSOD; Reactive oxygen species generation; Superoxide dismutase

Indexed keywords

ANTIOXIDANT; CELLFOOD; HYPOXIA INDUCIBLE FACTOR 1ALPHA; MANGANESE SUPEROXIDE DISMUTASE; REACTIVE OXYGEN METABOLITE; UNCLASSIFIED DRUG;

ANTIOXIDANT ACTIVITY; ARTICLE; CELL ACTIVITY; CELL VIABILITY; CONTROLLED STUDY; CYTOPLASM; DRUG EFFICACY; ENDOTHELIUM CELL; ENZYME ACTIVITY; HUMAN; HUMAN CELL; HYPOXIA; MITOCHONDRIAL RESPIRATION; OXYGEN CONSUMPTION; OXYGEN SUPPLY; PROTEIN EXPRESSION; PROTEIN LOCALIZATION; UMBILICAL VEIN ENDOTHELIAL CELL; UPREGULATION;

ADENOSINE TRIPHOSPHATE; AMINO ACIDS; ANTIOXIDANTS; CELL HYPOXIA; CELL RESPIRATION; CELL SURVIVAL; ENDOTHELIAL CELLS; ENZYMES; HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS; HUMANS; HYPOXIA-INDUCIBLE FACTOR 1, ALPHA SUBUNIT; L-LACTATE DEHYDROGENASE; MINERALS; MITOCHONDRIA; OXIDATION-REDUCTION; OXYGEN CONSUMPTION; REACTIVE OXYGEN SPECIES; SULFATES; SUPEROXIDE DISMUTASE;

EID: 80355143539     PISSN: 08675910     EISSN: None     Source Type: Journal    
DOI: None     Document Type: Article

References (31)

1. Aird WC. Endothelium in health and disease. Pharmacol Rep 2008; 60: 139-143.
2. Aragones J, Fraisl P, Baes M, Carmeliet P. Oxygen sensors at the crossroad of metabolism. Cell Metab 2009; 9: 11-22.
3. Veschini L, Belloni D, Foglieni C, et al. Hypoxia-inducible transcription factor-1 alpha determines sensitivity of endothelial cells to the proteosome inhibitor bortezomib. Blood 2007; 109: 2565-2570.
4. Dranka BP, Hill BG, Darley-Usmar VM. Mitochondrial reserve capacity in endothelial cells: the impact of nitric oxide and reactive oxygen species. Free Radic Biol Med 2010; 48: 905-914.
5. Davidson SM, Duchen MR. Endothelial mitochondria: contributing to vascular function and disease. Circ Res 2007; 100: 1128-1141.
6. Clementi E, Brown GC, Foxwell N, Moncada S. On the mechanism by which vascular endothelial cells regulate their oxygen consumption. Proc Natl Acad Sci USA 1991; 96: 1559-1562.
7. Heistad DD. Oxidative stress and vascular disease: 2005 Duff lecture. Arterioscler Thromb Vasc Biol 2006; 26: 689-695.
8. Pagano PJ, Ito Y, Tornheim K, Gallop PM, Tauber AI, Cohen RA. An NADPH oxidase superoxide-generating system in the rabbit aorta. Am J Physiol 1995; 268: H2274-H2280.
9. Culic O, Gruwel ML, Schrader J. Energy turnover of vascular endothelial cells. Am J Physiol 1997; 273: C205-C213.
10. Loike JD, Cao L, Brett J, Ogawa S, Silverstein SC, Stern D. Hypoxia induces glucose transporter expression in endothelial cells. Am J Physiol 1992; 263: C326-C333.
11. Madamanchi NR, Runge MS. Mitochondrial dysfunction in atherosclerosis. Circ Res 2007; 100: 460-473.
12. Ferrero E, Ferrero ME, Pardi R, Zocchi MR. The platelet endothelial cell adhesion molecule-1 (PECAM1) contributes to endothelial barrier function. FEBS Lett 1995; 374: 323-326.
13. CRC Handbook of Chemistry and Physics. D.R. Lide (ed)., Cleveland, Chemical Rubber Pub. Co., 2008.
14. Yang NC, Ho WM, Chen YH, Hu ML. A convenient onestep extraction of cellular ATP using boiling water for the luciferin-luciferase assay of ATP. Anal Biochem 2002; 306: 323-327.
15. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 1976; 72: 248-254.
16. Sweet IR, Gilbert M, Maloney E, Hockenbery DM, Schwartz MW, Kim F. Endothelial inflammation induced by excess glucose is associated with cytosolic glucose 6-phosphate but not increased mitochondrial respiration. Diabetologia 2009; 52: 921-931.
17. Gao L, Mann GE. Vascular NAD(P)H oxidase activation in diabetes: a double-edged sword in redox signalling. Cardiovasc Res 2009; 82: 9-20.
18. Lee MY, Griendling KK. Redox signaling, vascular function, and hypertension. Antioxid Redox Signal 2008; 10: 1045-1059.
19. Kaelin WG, Ratcliffe PJ. Oxygen sensing by metazoans: the central role of the HIF hydroxylase pathway. Mol Cell 2008; 30: 393-402.
20. Ushio-Fukai M, Nakamura Y. Reactive oxygen species and angiogenesis: NADPH oxidase as target for cancer therapy. Cancer Lett 2008; 266: 37-52.
21. Pugh CW, Ratcliffe PJ. Regulation of angiogenesis by hypoxia: role of the HIF system. Nat Med 2003; 9: 677-684.
22. Roquer J, Segura T, Serena J, Castillo J. Endothelial dysfunction, vascular disease and stroke: the ARTICO study. Cerebrovasc Dis 2009; 27(Suppl 1): 25-37.
23. Esper RJ, Nordaby RA, Vilarino JO, Paragano A, Cacharron JL, Machado RA. Endothelial dysfunction: a comprehensive appraisal. Cardiovasc Diabetol 2006; 5: 4.
24. Zapolska-Downar D, Naruszewicz M. Propionate reduces the cytokine-induced VCAM-1 and ICAM-1 expression by inhibiting nuclear factor-kappa B (NF-kappaB) activation. J Physiol Pharmacol 2009; 60: 123-131.
25. Gawad A, Ptak-Belowska A, Brzozowski T, Pawlik WW. Monocytes and vascular endothelial cells apoptosis. Role of p-HSP27. J Physiol Pharmacol 2009; 60: 55-61.
26. Dernbach E, Urbich C, Brandes RP, Hofmann WK, Zeiher AM, Dimmeler S. Antioxidative stress-associated genes in circulating progenitor cells: evidence for enhanced resistance against oxidative stress. Blood 2004; 104: 3591-3597.
27. Spanier G, Xu H, Xia N, et al. Resveratrol reduces endothelial oxidative stress by modulating the gene expression of superoxide dismutase 1 (SOD1), glutathione peroxidase 1 (GPx1) and NADPH oxidase subunit (Nox4). J Physiol Pharmacol 2009; 60(Suppl 4): 111-116.
28. Fukai T, Ushio-Fukai M. Superoxide dismutases: role in redox signaling, vascular function and diseases. Antioxid Redox Signal 2011; June 6, epub. ahead of print.
29. Miller JD, Peotta VA, Chu Y, Weiss RM, Zimmerman K, Brooks RM, et al. MnSOD protects against COX1-mediated endothelial dysfunction in chronic heart failure. Am J Physiol Heart Circ Physiol 2010; 298(5): H1600-H1607.
30. Methy D, Bertrand N, Prigent-Tessier A, Stanimirovic D, Beley A, Marie C. Differential MnSOD and HO-1 expression in cerebral endothelial cells in response to sublethal oxidative stress. Brain Res 2004; 1003: 151-158.
31. Fijalkowska I, Xu W, Comhair SA, et al. Hypoxia induciblefactor1alpha regulates the metabolic shift of pulmonary hypertensive endothelial cells. Am J Pathol 2010; 176: 1130-1138.