A method for murine islet isolation and subcapsular kidney transplantation

Journal of Visualized Experiments

Volumn , Issue 50, 2011, Pages

  Zmuda, Erik J ^a   Powell, Catherine A ^a   Hai, Tsonwin ^a  

^a OHIO STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355139363     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2096     Document Type: Article

References (14)

1. Shapiro, A. M. et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 343, 230-238 (2000).
2. Weir, G. C. & Bonner-Weir, S. Islet transplantation as a treatment for diabetes. J Am Optom Assoc 69, 727-732 (1998).
3. Gangemi, A. et al. title typecontributorIslet transplantation for brittle type 1 diabetes: the UIC protocol/title. Am J Transplant 8, 1250-1261, doi:AJT2234 [pii] 10.1111/j.1600-6143.2008.02234.x (2008).
4. Zmuda, E. et al. Deficiency of ATF3, an adaptive-response gene, protects islets and ameliorates inflammation in a syngenic transplantation model. Diabetologia Accpeted, preparing for press. (2010).
5. Bottino, R. et al. title typecontributorResponse of Human Islets to Isolation Stress and the Effect of Antioxidant Treatment/title. Diabetes 53, 2559-2568, doi:10.2337/diabetes.53.10.2559 (2004).
6. Markmann, J. F. et al. The effect of islet cell culture in vitro at 24 degrees C on graft survival and MHC antigen expression. Transplantation 49, 272-277 (1990).
7. Terasaka, R., Lacy, P. E., Hauptfeld, V., Bucy, R. P. & Davie, J. M. The effect of cyclosporin-A, low-temperature culture, and anti-Ia antibodies on prevention of rejection of rat islet allografts. Diabetes 35, 83-88 (1986).
8. Ricordi, C., Lacy, P. E., Sterbenz, K. & Davie, J. M. Low-temperature culture of human islets or in vivo treatment with L3T4 antibody produces a marked prolongation of islet human-to-mouse xenograft survival. Proc Natl Acad Sci U S A 84, 8080-8084 (1987).
9. Schnedl, W. J., Ferber, S., Johnson, J. H. & Newgard, C. B. STZ transport and cytotoxicity. Specific enhancement in GLUT2-expressing cells. Diabetes 43, 1326-1333 (1994).
10. Eckhard, M., Brandhorst, D., Brandhorst, H., Brendel, M. D. & Bretzel, R. G. Optimization in osmolality and range of density of a continuous ficoll-sodium-diatrizoate gradient for isopycnic purification of isolated human islets. Transplantation Proceedings 36, 2849-2854 (2004).
11. Gores, P. F., Rabe, F. & Sutherland, D. E. Prolonged survival of intraportal versus subrenal capsular transplanted islet allografts. Transplantation 43, 747-749 (1987).
12. Nasr, I. W. et al. Testicular Immune Privilege Promotes Transplantation Tolerance by Altering the Balance between Memory and Regulatory T Cells. J Immunol 174, 6161-6168 (2005).
13. Merani, S., Toso, C., Emamaullee, J. & Shapiro, A. M. J. Optimal implantation site for pancreatic islet transplantation. British Journal of Surgery 95, 1449-1461 (2008).
14. Inoue, M., Maeno, T. & Hatchell, D. L. title typecontributorSurvival of allografted pancreatic islets in the subretinal space in rats/title. Ophthalmic Res 35, 48-53, doi:10.1159/000068197 ore35048 [pii] (2003).