Identification and analysis of mouse erythroid progenitors using the CD71/TER119 flow-cytometric assay

Journal of Visualized Experiments

Volumn , Issue 54, 2011, Pages

  Koulnis, Miroslav ^a   Pop, Ramona ^a   Porpiglia, Ermelinda ^a   Shearstone, Jeffrey R ^a   Hidalgo, Daniel ^a   Socolovsky, Merav ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

CD71; Cell cycle; Developmental Biology; EpoR mouse; Erythroblast; Erythroid subsets; Erythropoiesis; Erythropoietic stress; Erythropoietin; Fetal erythropoiesis; Fetal liver; Flow cytometry; Hematopoietic progenitors; Issue 54; Ter119

Indexed keywords

EID: 80355132656     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2809     Document Type: Article

References (11)

1. Liu, Y., et al. Suppression of Fas-FasL coexpression by erythropoietin mediates erythroblast expansion during the erythropoietic stress response in vivo. Blood. 108, 123-33 (2006).
2. Socolovsky, M., et al. Negative Autoregulation by FAS Mediates Robust Fetal Erythropoiesis. PLoS Biol. 5, e252 (2007).
3. Krutzik, P.O., Hale, M.B., & Nolan, G.P. Characterization of the murine immunological signaling network with phosphospecific flow cytometry. J Immunol. 175, 2366-73 (2005).
4. Socolovsky, M., et al. Ineffective erythropoiesis in Stat5a(-/-)5b(-/-) mice due to decreased survival of early erythroblasts. Blood. 98, 3261-73 (2001).
5. Guihard, S., et al. The MAPK ERK1 is a negative regulator of the adult steady-state splenic erythropoiesis. Blood. 115, 3686-94 (2010).
6. Yu, X., et al. An erythroid chaperone that facilitates folding of alpha-globin subunits for hemoglobin synthesis. J Clin Invest. 117, 1856-65 (2007).
7. Chen, M.L., et al. Erythroid dysplasia, megaloblastic anemia, and impaired lymphopoiesis arising from mitochondrial dysfunction. Blood. 114, 4045-53 (2009).
8. Chen, K., et al. Resolving the distinct stages in erythroid differentiation based on dynamic changes in membrane protein expression during erythropoiesis. Proc Natl Acad Sci U S A. 106, 17413-8 (2009).
9. McGrath, K.E., Bushnell, T.P., & Palis, J. Multispectral imaging of hematopoietic cells: where flow meets morphology. J Immunol Methods. 336, 91-7 (2008).
10. Pop, R. et al. A key commitment step in erythropoiesis is synchronized with the cell cycle clock through mutual inhibition between PU.1 and S-phase progression. PLoS Biol. 8, (2010).
11. Borsook, H., Lingrel, J.B., Scaro, J.L., & Millette, R.L. Synthesis of haemoglobin in relation to the maturation of erythroid cells. Nature. 196, 347-50 (1962).