Development of a negative selectable marker for Entamoeba histolytica

Journal of Visualized Experiments

Volumn , Issue 46, 2010, Pages

  Abhyankar, Mayuresh M ^a   Haviland, Sarah M ^a   Gilchrist, Carol A ^a   Petri Jr , William A ^a  

^a UNIVERSITY OF VIRGINIA HEALTH SYSTEM   (United States)

Author keywords

5 fluorocytosine; Cytosine deaminase; Entamoeba; Gene knockout; Infectious Disease; Issue 46; Negative selectable marker; UPRT CMFDA

Indexed keywords

EID: 80355126783     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2410     Document Type: Article

References (12)

1. Olvera, A. et al. Stable transfection of Entamoeba histolytica trophozoites by lipofection. Arch. Med. Res 28 Spec No, 49-51 (1997).
2. Saito-Nakano, Y., Yasuda, T., Nakada-Tsukui, K., Leippe, M. & Nozaki, T. Rab5-associated vacuoles play a unique role in phagocytosis of the enteric protozoan parasite Entamoeba histolytica. J. Biol. Chem 279, 49497-49507 (2004).
3. Diamond, L.S., Harlow, D.R. & Cunnick, C.C. A new medium for the axenic cultivation of Entamoeba histolytica and other Entamoeba. Trans. R. Soc. Trop. Med. Hyg 72, 431-432 (1978).
4. Clark, C. Anaerobic parasitic protozoa: genomics and molecular biology. (Caister Academic: Norfolk UK, 2010).
5. Linford, A.S. et al. Short hairpin RNA-mediated knockdown of protein expression in Entamoeba histolytica. BMC Microbiol 9, 38 (2009).
6. Solis, C.F. & Guillén, N. Silencing genes by RNA interference in the protozoan parasite Entamoeba histolytica. Methods Mol. Biol 442, 113-128 (2008).
7. Solis, C.F., Santi-Rocca, J., Perdomo, D., Weber, C. & Guillén, N. Use of bacterially expressed dsRNA to downregulate Entamoeba histolytica gene expression. PLoS ONE 4, e8424 (2009).
8. MacFarlane, R.C. & Singh, U. Loss of dsRNA-based gene silencing in Entamoeba histolytica: implications for approaches to genetic analysis. Exp. Parasitol 119, 296-300 (2008).
9. Dhar, S.K., Vines, R.R., Bhattacharya, S. & Petri, W.A. Ribosomal DNA fragments enhance the stability of transfected DNA in Entamoeba histolytica. J. Eukaryot. Microbiol 45, 656-660 (1998).
10. Erbs, P. et al. In vivo cancer gene therapy by adenovirus-mediated transfer of a bifunctional yeast cytosine deaminase/uracil phosphoribosyltransferase fusion gene. Cancer Res 60, 3813-3822 (2000).
11. Braks, J.A.M., Franke-Fayard, B., Kroeze, H., Janse, C.J. & Waters, A.P. Development and application of a positive-negative selectable marker system for use in reverse genetics in Plasmodium. Nucleic Acids Res 34, e39 (2006).
12. Maier, A.G., Braks, J.A.M., Waters, A.P. & Cowman, A.F. Negative selection using yeast cytosine deaminase/uracil phosphoribosyl transferase in Plasmodium falciparum for targeted gene deletion by double crossover recombination. Mol. Biochem. Parasitol 150, 118-121 (2006).