Development of a multiplex PCR assay targeting O-antigen modification genes for molecular serotyping of Shigella flexneri

Journal of Clinical Microbiology

Volumn 49, Issue 11, 2011, Pages 3766-3770

  Sun, Qiangzheng ^a   Lan, Ruiting ^b   Wang, Yiting ^a   Zhao, Ailan ^a   Zhang, Shaomin ^a   Wang, Jianping ^a   Wang, Yan ^a   Xia, Shengli ^c   Jin, Dong ^a   Cui, Zhigang ^a   Zhao, Hongqing ^a   Li, Zhenjun ^a   Ye, Changyun ^a   Zhang, Shuxia ^a   Jing, Huaiqi ^a   Xu, Jianguo ^a  

^a National Institute for Communicable Disease Control and Prevention   (China)

^b UNIVERSITY OF NEW SOUTH WALES   (Australia)

^c CHINESE CENTER FOR DISEASE CONTROL AND PREVENTION   (China)

Author keywords

[No Author keywords available]

Indexed keywords

O ANTIGEN;

ANTIGEN ANTIBODY REACTION; ARTICLE; BACTERIAL GENE; BACTERIUM IDENTIFICATION; CONTROLLED STUDY; DNA MODIFICATION; GENETIC VARIABILITY; GTRI GENE; GTRIC GENE; GTRII GENE; GTRIV GENE; GTRV GENE; GTRX GENE; MOLECULAR GENETICS; MULTIPLEX POLYMERASE CHAIN REACTION; NONHUMAN; NUCLEOTIDE SEQUENCE; OAC GENE; PRIORITY JOURNAL; PROTEIN SYNTHESIS; SEROTYPE; SHIGELLA FLEXNERI;

AGGLUTINATION TESTS; HUMANS; MEMBRANE TRANSPORT PROTEINS; METABOLIC NETWORKS AND PATHWAYS; MULTIPLEX POLYMERASE CHAIN REACTION; O ANTIGENS; SEROTYPING; SHIGELLA FLEXNERI;

SHIGELLA; SHIGELLA FLEXNERI;

EID: 80355126243     PISSN: 00951137     EISSN: 1098660X     Source Type: Journal    
DOI: 10.1128/JCM.01259-11     Document Type: Article

References (21)

1. Adams, M. M., G. E. Allison, and N. K. Verma. 2001. Type IV O antigen modification genes in the genome of Shigella flexneri NCTC 8296. Microbiology 147:851-860. (Pubitemid 32401325)
2. Adhikari, P., G. Allison, B. Whittle, and N. K. Verma. 1999. Serotype 1a O-antigen modification: molecular characterization of the genes involved and their novel organization in the Shigella flexneri chromosome. J. Bacteriol. 181:4711-4718. (Pubitemid 29357962)
3. Allison, G. E., and N. K. Verma. 2000. Serotype-converting bacteriophages and O-antigen modification in Shigella flexneri. Trends Microbiol. 8:17-23. (Pubitemid 30066379)
4. Cheah, K. C., D. W. Beger, and P. A. Manning. 1991. Molecular cloning and genetic analysis of the rfb region from Shigella flexneri type 6 in Escherichia coli K-12. FEMS Microbiol. Lett. 67:213-218.
5. Chen, J. H., W. B. Hsu, C. S. Chiou, and C. M. Chen. 2003. Conversion of Shigella flexneri serotype 2a to serotype Y in a shigellosis patient due to a single amino acid substitution in the protein product of the bacterial glucosyltransferase gtrII gene. FEMS Microbiol. Lett. 224:277-283.
6. Clark, C. A., J. Beltrame, and P. A. Manning. 1991. The oac gene encoding a lipopolysaccharide O-antigen acetylase maps adjacent to the integraseencoding gene on the genome of Shigella flexneri bacteriophage Sf6. Gene 107:43-52.
7. Forster, R. A., et al. Structural elucidation of the O-antigen of the Shigella flexneri provisional serotype 88-893: structural and serological similarities with S. flexneri provisional serotype Y394 (1c). Carbohydr. Res. 346:872-876.
8. Guan, S., D. A. Bastin, and N. K. Verma. 1999. Functional analysis of the O antigen glucosylation gene cluster of Shigella flexneri bacteriophage SfX. Microbiology 145:1263-1273. (Pubitemid 29231831)
9. Huan, P. T., D. A. Bastin, B. L. Whittle, A. A. Lindberg, and N. K. Verma. 1997. Molecular characterization of the genes involved in O-antigen modification, attachment, integration and excision in Shigella flexneri bacteriophage SfV. Gene 195:217-227. (Pubitemid 27354699)
10. Kotloff, K. L., et al. 1999. Global burden of Shigella infections: implications for vaccine development and implementation of control strategies. Bull. World Health Organ. 77:651-666. (Pubitemid 29446617)
11. Li, Y., et al. 2009. Molecular detection of all 34 distinct O-antigen forms of Shigella. J. Med. Microbiol. 58:69-81.
12. Mavris, M., P. A. Manning, and R. Morona. 1997. Mechanism of bacteriophage SfII-mediated serotype conversion in Shigella flexneri. Mol. Microbiol. 26:939-950. (Pubitemid 27507878)
13. Pryamukhina, N. S., and N. A. Khomenko. 1988. Suggestion to supplement Shigella flexneri classification scheme with the subserovar Shigella flexneri 4c: phenotypic characteristics of strains. J. Clin. Microbiol. 26:1147-1149.
14. Roberts, F., A. V. Jennison, and N. K. Verma. 2005. The Shigella flexneri serotype Y vaccine candidate SFL124 originated from a serotype 2a background. FEMS Immunol. Med. Microbiol. 45:285-289. (Pubitemid 41040013)
15. Simmons, D. A., and E. Romanowska. 1987. Structure and biology of Shigella flexneri O antigens. J. Med. Microbiol. 23:289-302. (Pubitemid 17099384)
16. Stagg, R. M., P. D. Cam, and N. K. Verma. 2008. Identification of newly recognized serotype 1c as the most prevalent Shigella flexneri serotype in northern rural Vietnam. Epidemiol. Infect. 136:1134-1140. (Pubitemid 351982937)
17. Stagg, R. M., et al. 2009. A novel glucosyltransferase involved in O-antigen modification of Shigella flexneri serotype 1c. J. Bacteriol. 191:6612-6617.
18. Talukder, K. A., et al. 2003. Phenotypic and genotypic characterization of provisional serotype Shigella flexneri 1c and clonal relationships with 1a and 1b strains isolated in Bangladesh. J. Clin. Microbiol. 41:110-117. (Pubitemid 36098563)
19. Verma, N. K., J. M. Brandt, D. J. Verma, and A. A. Lindberg. 1991. Molecular characterization of the O-acetyl transferase gene of converting bacteriophage SF6 that adds group antigen 6 to Shigella flexneri. Mol. Microbiol. 5:71-75. (Pubitemid 21895942)
20. von Seidlein, L., et al. 2006. A multicentre study of Shigella diarrhoea in six Asian countries: disease burden, clinical manifestations, and microbiology. PLoS Med. 3:e353.
21. Ye, C., et al. 2010. Emergence of a new multidrug-resistant serotype X variant in an epidemic clone of Shigella flexneri. J. Clin. Microbiol. 48:419-426.