Multiplex PCR and reverse line blot hybridization assay (mPCR/RLB)

Journal of Visualized Experiments

Volumn , Issue 54, 2011, Pages

  O'Sullivan, Matthew V N ^a   Zhou, Fei ^a   Sintchenko, Vitali ^a   Kong, Fanrong ^a   Gilbert, Gwendolyn L ^a  

^a UNIVERSITY OF SYDNEY   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 80355125182     PISSN: 1940087X     EISSN: None     Source Type: Journal    
DOI: 10.3791/2781     Document Type: Article

References (19)

1. O'Sullivan, M.V., Cai, Y., Kong, F., Zeng, X., & Gilbert, G.L. Influence of disk separation distance on accuracy of the disk approximation test for detection of inducible clindamycin resistance in Staphylococcus spp. J Clin Microbiol. 44, 4072-4076 (2006).
2. Zeng, X., Kong, F., Wang, H., Darbar, A., & Gilbert, G.L. Simultaneous detection of nine antibiotic resistance-related genes in Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization assay. Antimicrob Agents Chemother. 50, 204-209 (2006).
3. Cai, Y., et al. Comparison of single- and multilocus sequence typing and toxin gene profiling for characterization of methicillin-resistant Staphylococcus aureus. J Clin Microbiol. 45, 3302-3308 (2007).
4. Cai, L., et al. A new multiplex PCR-based reverse line-blot hybridization (mPCR/RLB) assay for rapid staphylococcal cassette chromosome mec (SCCmec) typing. J Med Microbiol. 58, 1045-1057 (2009).
5. O'Sullivan, M.V., Kong, F., Sintchenko, V., & Gilbert, G.L. Rapid identification of methicillin-resistant Staphylococcus aureus transmission in hospitals by use of phage-derived open reading frame typing enhanced by multiplex PCR and reverse line blot assay. J Clin Microbiol. 48, 2741-2748 (2010).
6. Wang, Q., Kong, F., Jelfs, P., & Gilbert, G.L. Extended phage locus typing of Salmonella enterica serovar Typhimurium, using multiplex PCR-based reverse line blot hybridization. J Med Microbiol. 57, 827-838 (2008).
7. Zhou, F., Kong, F., Tong, Z., & Gilbert, G.L. Identification of less-common Streptococcus pneumoniae serotypes by a multiplex PCR-based reverse line blot hybridization assay. J Clin Microbiol. 45, 3411-3415 (2007).
8. Kong, F., Brown, M., Sabananthan, A., Zeng, X., & Gilbert, G.L. Multiplex PCR-based reverse line blot hybridization assay to identify 23 Streptococcus pneumoniae polysaccharide vaccine serotypes. J Clin Microbiol. 44, 1887-1891 (2006).
9. Kong, F., Ma, L., & Gilbert, G.L. Simultaneous detection and serotype identification of Streptococcus agalactiae using multiplex PCR and reverse line blot hybridization. J Med Microbiol. 54, 1133-1138 (2005).
10. Zhou, F., et al. Identification of 20 common human enterovirus serotypes by use of a reverse transcription-PCR-based reverse line blot hybridization assay. J Clin Microbiol. 47, 2737-2743 (2009).
11. Zhou, F., et al. Molecular identification and analysis of nonserotypeable human enteroviruses. J Clin Microbiol. 48, 1276-1282 (2010).
12. Xiong, L., Kong, F., Yang, Y., Cheng, J., & Gilbert, G.L. Use of PCR and reverse line blot hybridization macroarray based on 16S-23S rRNA gene internal transcribed spacer sequences for rapid identification of 34 mycobacterium species. J Clin Microbiol. 44, 3544-3550 (2006).
13. Wang, H., Kong, F., Wang, B., McKechnie, M.L., & Gilbert, G.L. Multiplex polymerase chain reaction-based reverse line blot hybridization assay to detect common genital pathogens. Int J STD AIDS. 21, 320-325 (2010).
14. McKechnie, M.L., et al. Simultaneous identification of 14 genital microorganisms in urine by use of a multiplex PCR-based reverse line blot assay. J Clin Microbiol. 47, 1871-1877 (2009).
15. Xiong, L., Kong, F., Zhou, H. & Gilbert, G.L. Use of PCR and reverse line blot hybridization assay for rapid simultaneous detection and serovar identification of Chlamydia trachomatis. J Clin Microbiol. 44, 1413-1418 (2006).
16. Wang, Y., Kong, F., Yang, Y. & Gilbert, G.L. A multiplex PCR-based reverse line blot hybridization (mPCR/RLB) assay for detection of bacterial respiratory pathogens in children with pneumonia. Pediatr Pulmonol. 43, 150-159 (2008).
17. Wang, Y., et al. Use of a multiplex PCR-based reverse line blot (mPCR/RLB) hybridisation assay for the rapid identification of bacterial pathogens. Clin Microbiol Infect. 14, 155-160 (2008).
18. Wang, H., Kong, F., Jelfs, P., James, G., & Gilbert, G.L. Simultaneous detection and identification of common cell culture contaminant and pathogenic mollicutes strains by reverse line blot hybridization. Appl Environ Microbiol. 70, 1483-1486 (2004).
19. Kong, F. & Gilbert, G.L. Multiplex PCR-based reverse line blot hybridization assay (mPCR/RLB) - a practical epidemiological and diagnostic tool. Nat Protoc. 1, 2668-2680 (2006).