Simultaneous Detection and Identification of Common Cell Culture Contaminant and Pathogenic Mollicutes Strains by Reverse Line Blot Hybridization

Applied and Environmental Microbiology

Volumn 70, Issue 3, 2004, Pages 1483-1486

  Wang, Hui ^a,c   Kong, Fanrong ^a,b   Jelfs, Peter ^a   James, Gregory ^a   Gilbert, Gwendolyn L ^a,b,d  

^a Western Sydney Area Health Service   (Australia)

^b UNIVERSITY OF SYDNEY   (Australia)

^c First Hospital of Wuhan   (China)

^d WESTMEAD HOSPITAL   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

BIOASSAY; BIOLOGICAL MEMBRANES; CONTAMINATION; ENZYME KINETICS; RNA;

HYBRIDIZATION ASSAYS;

CELL CULTURE;

BIOTIN; MYCOPLASMA ARGININE; RNA 16S; RNA 23S; SPACER DNA; UNCLASSIFIED DRUG;

DETECTION METHOD; IDENTIFICATION METHOD; MICROBIOLOGY;

ACHOLEPLASMA LAIDLAWII; AMPLICON; ARTICLE; BACTERIUM ISOLATE; CELL CULTURE; CONTAMINATION; CONTROLLED STUDY; HUMAN; HUMAN CELL; HYBRIDIZATION; INTERMETHOD COMPARISON; MICROBIAL IDENTIFICATION; MOLLICUTES; MYCOPLASMA; MYCOPLASMA GENITALIUM; MYCOPLASMA HOMINIS; MYCOPLASMA HYORHINIS; MYCOPLASMA ORALE; MYCOPLASMA PNEUMONIAE; NONHUMAN; NUCLEOTIDE SEQUENCE; OLIGONUCLEOTIDE PROBE; POLYMERASE CHAIN REACTION; REVERSE LINE BLOT HYBRIDIZATION; RNA SEQUENCE; SENSITIVITY AND SPECIFICITY; STANDARD; UREAPLASMA PARVUM; UREAPLASMA UREALYTICUM;

ACHOLEPLASMA; BASE SEQUENCE; CELL CULTURE TECHNIQUES; DNA PRIMERS; DNA, RIBOSOMAL SPACER; HUMANS; MOLLICUTES; MYCOPLASMA; NUCLEIC ACID HYBRIDIZATION; POLYMERASE CHAIN REACTION; RNA, BACTERIAL; RNA, RIBOSOMAL, 16S; RNA, RIBOSOMAL, 23S; SENSITIVITY AND SPECIFICITY; UREAPLASMA;

ACHOLEPLASMA LAIDLAWII; BACTERIA (MICROORGANISMS); MOLLICUTES; MYCOPLASMA; MYCOPLASMA ARGININI; MYCOPLASMA FERMENTANS; MYCOPLASMA GENITALIUM; MYCOPLASMA HOMINIS; MYCOPLASMA HYORHINIS; MYCOPLASMA ORALE; MYCOPLASMA PNEUMONIAE; PROKARYOTA; UREAPLASMA PARVUM; UREAPLASMA UREALYTICUM;

EID: 1642335610     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/AEM.70.3.1483-1486.2004     Document Type: Article

References (22)

1. Blazek, R., K. Schmitt, U. Krafft, and U. Hadding. 1990. Fast and simple procedure for the detection of cell culture mycoplasmas using a single monoclonal antibody. J. Immunol. Methods 131:203-212.
2. Deguchi, T., and S. Maeda. 2002. Mycoplasma genitalium: another important pathogen of nongonococcal urethritis. J. Urol. 167:1210-1217.
3. Gubbels, J. M., A. P. de Vos, M. van der Weide, J. Viseras, L. M. Schouls, E. de Vries, and F. Jongejan. 1999. Simultaneous detection of bovine Theileria and Babesia species by reverse line blot hybridization. J. Clin. Microbiol. 37:1782-1789.
4. Hammerschlag, R. 2001. Mycoplasma pneumoniae infections. Curr. Opin. Infect. Dis. 14:181-186.
5. Harasawa, R., H. Hotzel, and K. Sachse. 2000. Comparison of the 16S-23S rRNA intergenic spacer regions among strains of the Mycoplasma mycoides cluster, and reassessment of the taxonomic position of Mycoplasma sp. bovine group 7. Int. J. Syst. Evol. Microbiol. 50:1325-1329.
6. Harasawa, R., H. Mizusawa, K. Nozawa, T. Nakagawa, K. Asada, and I. Kato. 1993. Detection and tentative identification of dominant mycoplasma species in cell cultures by restriction analysis of the 16S-23S rRNA intergenic spacer regions. Res. Microbiol. 144:489-493.
7. Kong, F., S. Gordon, and G. L. Gilbert. 2000. Rapid-cycle PCR for detection and typing of Mycoplasma pneumoniae in clinical specimens. J. Clin. Microbiol. 38:4256-4259.
8. Kong, F., G. James, S. Gordon, A. Zelynski, and G. L. Gilbert. 2001. Species-specific PCR for identification of common contaminant mollicutes in cell culture. Appl. Environ. Microbiol. 67:3195-3200.
9. Kong, F., Z. Ma, G. James, S. Gordon, and G. L. Gilbert. 2000. Species identification and subtyping of Ureaplasma parvum and Ureaplasma urealyticum by using PCR-based assays. J. Clin. Microbiol. 38:1175-1179.
10. Kong, F., X. Zhu, W. Wang, X. Zhou, S. Gordon, and G. L. Gilbert. 1999. Comparative analysis and serovar-specific identification of multiple-banded antigen genes of Ureaplasma urealyticum biovar 1. J. Clin. Microbiol. 37:538-543.
11. Ladefoged, S. A. 2000. Molecular dissection of Mycoplasma hominis. APMIS Suppl. 97:1-45.
12. Mattsson, J. G., and K. E. Johansson. 1993. Oligonucleotide probes complementary to 16S rRNA for rapid detection of mycoplasma contamination in cell cultures. FEMS Microbiol. Lett. 107:139-144.
13. Ossewaarde, J. M., A. de Vries, T. Bestebroer, and A. F. Angulo. 1996. Application of a Mycoplasma group-specific PCR for monitoring decontamination of Mycoplasma-infected Chlamydia sp. strains. Appl. Environ. Microbiol. 62:328-331.
14. Payment, P., M. Corbeil, and A. Chagnon. 1978. Detection of Mycoplasma hominis and Mycoplasma orale in cell cultures by immunofluorescence. Can. J. Microbiol. 24:689-692.
15. Razin, S., D. Yogev, and Y. Naot. 1998. Molecular biology and pathogenicity of mycoplasmas. Microbiol. Mol. Biol. Rev. 62:1094-1156.
16. Robertson, J. A., G. W. Stemke, J. W. Davis, Jr., R. Harasawa, D. Thirkell, F. Kong, M. C. Shepard, and D. K. Ford. 2002. Proposal of Ureaplasma parvum sp. nov. and emended description of Ureaplasma urealyticum (Shepard et al. 1974) Robertson et al. 2001. Int. J. Syst. Evol. Microbiol. 52:587-597.
17. Tang, J., M. Hu, S. Lee, and R. Roblin. 2000. A polymerase chain reaction based method for detecting Mycoplasma/Acholeplasma contaminants in cell culture. J. Microbiol. Methods 39:121-126.
18. Taylor-Robinson, D. 2002. Mycoplasma genitalium - an up-date. Int. J. STD AIDS 13:145-151.
19. Uphoff, C. C., and H. G. Drexler. 2002. Comparative PCR analysis for detection of mycoplasma infections in continuous cell lines. In Vitro Cell Dev. Biol. Anim. 38:79-85.
20. van Kuppeveld, F. J., K. E. Johansson, J. M. Galama, J. Kissing, G. Bolske, J. T. van der Logt, and W. J. Melchers. 1994. Detection of mycoplasma contamination in cell cultures by a mycoplasma group-specific PCR. Appl. Environ. Microbiol. 60:149-152.
21. Vinje, J., and M. P. Koopmans. 2000. Simultaneous detection and genotyping of "Norwalk-like viruses" by oligonucleotide array in a reverse line blot hybridization format. J. Clin. Microbiol. 38:2595-2601.
22. Yoshida, T., S. I. Maeda, T. Deguchi, T. Miyazawa, and H. Ishiko. 2003. Rapid detection of Mycoplasma genitalium, Mycoplasma hominis, Ureaplasma parvum, and Ureaplasma urealyticum organisms in genitourinary samples by PCR-microtiter plate hybridization assay. J. Clin. Microbiol. 41:1850-1855.