Asparagine-linked glycosylation of human chymotrypsin C is required for folding and secretion but not for enzyme activity

FEBS Journal

Volumn 278, Issue 22, 2011, Pages 4338-4350

  Bence, Melinda ^a   Sahin Tõth, Miklõs ^a  

^a BOSTON UNIVERSITY   (United States)

Author keywords

Asn linked glycosylation; ER stress; misfolding; pancreatic chymotrypsin; secretion defect; serine protease

Indexed keywords

ASPARAGINE; CHYMOTRYPSIN; CHYMOTRYPSIN C; ENZYME PRECURSOR; GLYCAN; GLYCOPEPTIDASE; GLYCOSIDASE; UNCLASSIFIED DRUG;

ACINAR CELL; ANIMAL CELL; ARTICLE; BINDING AFFINITY; BINDING SITE; CONTROLLED STUDY; ENDOPLASMIC RETICULUM STRESS; ENZYME ACTIVITY; MOLECULAR WEIGHT; NONHUMAN; PARTICLE SIZE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PROTEIN FOLDING; PROTEIN FUNCTION; PROTEIN GLYCOSYLATION; PROTEIN LOCALIZATION; PROTEIN SECRETION; RAT; RESIDUE ANALYSIS; SEQUENCE ANALYSIS; STRUCTURE ACTIVITY RELATION;

ANIMALS; ASPARAGINE; BLOTTING, WESTERN; CATTLE; CELLS, CULTURED; CHYMOTRYPSIN; ENDOPLASMIC RETICULUM; ENDOPLASMIC RETICULUM STRESS; GLYCOSYLATION; HUMANS; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; MUTATION; PANCREAS, EXOCRINE; PEPTIDE-N4-(N-ACETYL-BETA-GLUCOSAMINYL) ASPARAGINE AMIDASE; POLYSACCHARIDES; PROTEIN BINDING; PROTEIN FOLDING; RATS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA, MESSENGER;

MAMMALIA; RATTUS;

EID: 80255130597     PISSN: 1742464X     EISSN: 17424658     Source Type: Journal    
DOI: 10.1111/j.1742-4658.2011.08351.x     Document Type: Article

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