Engineering an L-cell line that expresses insulin under the control of the glucagon-like peptide-1 promoter for diabetes treatment

BMC Biotechnology

Volumn 11, Issue , 2011, Pages

  Rasouli, Mina ^a   Ahmad, Zalinah ^a   Omar, Abdul R ^a   Allaudin, Zeenathul N ^a  

^a UNIVERSITY PUTRA MALAYSIA   (Malaysia)

Author keywords

[No Author keywords available]

Indexed keywords

CLINICAL COMPLICATIONS; DIABETES MELLITUS; GLUCOSE CONCENTRATION; INSULIN GENE; INTESTINAL CELLS; ISOLATED CELLS; PATHOPHYSIOLOGY; PROTEIN SECRETION; RECOMBINANT PLASMID; SIDE EFFECT; TARGET CELLS;

CELL CULTURE; DISEASE CONTROL; DISEASES; GENE EXPRESSION; GENE THERAPY; GLUCOSE; INSULIN; PEPTIDES; PHYSIOLOGY;

CELLS;

GLUCAGON LIKE PEPTIDE 1; INSULIN; GLUCAGON; GLUCOSE;

ARTICLE; CELL ISOLATION; CONTROLLED STUDY; DIABETES MELLITUS; ENTEROENDOCRINE CELL; GENE EXPRESSION; GENE THERAPY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; L CELL; PROMOTER REGION; PROTEIN EXPRESSION; RECOMBINANT PLASMID; WESTERN BLOTTING; ANIMAL; CHEMISTRY; CYTOLOGY; DOSE RESPONSE; DRUG EFFECT; EPITHELIUM CELL; GENETIC ENGINEERING; GENETICS; INTESTINE; INTESTINE MUCOSA; METABOLISM; METHODOLOGY; MOLECULARLY TARGETED THERAPY; MOUSE; PLASMID; SECRETION; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; DIABETES MELLITUS; DOSE-RESPONSE RELATIONSHIP, DRUG; EPITHELIAL CELLS; GENE EXPRESSION; GENE THERAPY; GENETIC ENGINEERING; GLUCAGON; GLUCAGON-LIKE PEPTIDE 1; GLUCOSE; HUMANS; INSULIN; INTESTINAL MUCOSA; INTESTINES; MICE; MOLECULAR TARGETED THERAPY; PLASMIDS; PROMOTER REGIONS, GENETIC; TRANSFECTION;

EID: 80155201439     PISSN: None     EISSN: 14726750     Source Type: Journal    
DOI: 10.1186/1472-6750-11-99     Document Type: Article

References (23)

1. Yechoor V, Chan L. Gene therapy progress and prospects: gene therapy for diabetes mellitus. Gene Ther 2005, 12(2):101-107. 10.1038/sj.gt.3302412, 15496957.
2. Thule PM, Liu JM. Regulated hepatic insulin gene therapy of STZ-diabetic rats. Gene Ther 2000, 7(20):1744-1752. 10.1038/sj.gt.3301297, 11083496.
3. Yin D, Tang JG. Gene therapy for streptozotocin-induced diabetic mice by electroporational transfer of naked human insulin precursor DNA into skeletal muscle in vivo. FEBS Lett 2001, 495(1-2):16-20. 10.1016/S0014-5793(01)02352-3, 11322940.
4. Theodorakis MJ, Carlson O, Michopoulos S, Doyle ME, Juhaszova M, Petraki K, Egan JM. Human duodenal enteroendocrine cells: source of both incretin peptides, GLP-1 and GIP. Am J Physiol Endocrinol Metab 2006, 290(3):E550-559.
5. Lu F, Jin T, Drucker DJ. Proglucagon gene expression is induced by gastrin-releasing peptide in a mouse enteroendocrine cell line. Endocrinology 1996, 137(9):3710-3716. 10.1210/en.137.9.3710, 8756537.
6. Cheung AT, Dayanandan B, Lewis JT, Korbutt GS, Rajotte RV, Bryer-Ash M, Boylan MO, Wolfe MM, Kieffer TJ. Glucose-dependent insulin release from genetically engineered K cells. Science 2000, 290(5498):1959-1962. 10.1126/science.290.5498.1959, 11110661.
7. Ramshur EB, Rull TR, Wice BM. Novel insulin/GIP co-producing cell lines provide unexpected insights into Gut K-cell function in vivo. J Cell Physiol 2002, 192(3):339-350. 10.1002/jcp.10139, 12124779.
8. Tang SC, Sambanis A. Differential rAAV2 transduction efficiencies and insulin secretion profiles in pure and co-culture models of human enteroendocrine L-cells and enterocytes. J Gene Med 2004, 6(9):1003-1013. 10.1002/jgm.587, 15352073.
9. Bara H, Sambanis A. Insulin-secreting L-cells for the treatment of insulin-dependent diabetes. Biochem Biophys Res Commun 2008, 371(1):39-43. 10.1016/j.bbrc.2008.03.154, 2698788, 18406351.
10. Drucker DJ, Mojsov S, Habener JF. Cell-specific post-translational processing of preproglucagon expressed from a metallothionein-glucagon fusion gene. J Biol Chem 1986, 261(21):9637-9643.
11. Jin T, Drucker DJ. The proglucagon gene upstream enhancer contains positive and negative domains important for tissue-specific proglucagon gene transcription. Mol Endocrinol 1995, 9(10):1306-1320. 10.1210/me.9.10.1306, 8544839.
12. Rindi G, Grant SG, Yiangou Y, Ghatei MA, Bloom SR, Bautch VL, Solcia E, Polak JM. Development of neuroendocrine tumors in the gastrointestinal tract of transgenic mice. Heterogeneity of hormone expression. Am J Pathol 1990, 136(6):1349-1363. 1877573, 2162628.
13. Gosmain Y, Avril I, Mamin A, Philippe J. Pax-6 and c-Maf functionally interact with the alpha-cell-specific DNA element G1 in vivo to promote glucagon gene expression. J Biol Chem 2007, 282(48):35024-35034. 10.1074/jbc.M702795200, 17901057.
14. Han J, Lee HH, Kwon H, Shin S, Yoon JW, Jun HS. Engineered enteroendocrine cells secrete insulin in response to glucose and reverse hyperglycemia in diabetic mice. Mol Ther 2007, 15(6):1195-1202.
15. Chi Q, Huang K. Polyacrylamide Gel Electrophoresis of Insulin. Analytical Letters 2007, 40:95-102.
16. Dodson G, Steiner D. The role of assembly in insulin's biosynthesis. Curr Opin Struct Biol 1998, 8(2):189-194. 10.1016/S0959-440X(98)80037-7, 9631292.
17. Schagger H, von Jagow G. Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100 kDa. Anal Biochem 1987, 166(2):368-379. 10.1016/0003-2697(87)90587-2, 2449095.
18. Drucker DJ, Jin T, Asa SL, Young TA, Brubaker PL. Activation of proglucagon gene transcription by protein kinase-A in a novel mouse enteroendocrine cell line. Mol Endocrinol 1994, 8(12):1646-1655. 10.1210/me.8.12.1646, 7535893.
19. Zhang Y, Yao L, Shen K, Xu M, Zhou P, Yang W, Liu X, Qin X. Genetically engineered K cells provide sufficient insulin to correct hyperglycemia in a nude murine model. Acta Biochim Biophys Sin (Shanghai) 2008, 40(2):149-157.
20. Tang SC, Sambanis A. Development of genetically engineered human intestinal cells for regulated insulin secretion using rAAV-mediated gene transfer. Biochem Biophys Res Commun 2003, 303(2):645-652. 10.1016/S0006-291X(03)00399-1, 12659868.
21. Reimann F, Gribble FM. Glucose-sensing in glucagon-like peptide-1-secreting cells. Diabetes 2002, 51(9):2757-2763. 10.2337/diabetes.51.9.2757, 12196469.
22. Gribble FM, Williams L, Simpson AK, Reimann F. A novel glucose-sensing mechanism contributing to glucagon-like peptide-1 secretion from the GLUTag cell line. Diabetes 2003, 52(5):1147-1154. 10.2337/diabetes.52.5.1147, 12716745.
23. Reimann F, Williams L, da Silva Xavier G, Rutter GA, Gribble FM. Glutamine potently stimulates glucagon-like peptide-1 secretion from GLUTag cells. Diabetologia 2004, 47(9):1592-1601. 10.1007/s00125-004-1498-0, 15365617.