A polymerase chain reaction-based method for constructing a linear vector with site-specific DNA methylation

Analytical Biochemistry

Volumn 416, Issue 2, 2011, Pages 211-217

  Arakawa, Toshiya ^a   Ohta, Tohru ^a   Abiko, Yoshihiro ^a   Okayama, Miki ^a   Mizoguchi, Itaru ^a   Takuma, Taishin ^a  

^a HEALTH SCIENCES UNIVERSITY OF HOKKAIDO   (Japan)

Author keywords

DNA methylation; Site specific; Transcriptional regulation

Indexed keywords

ALKYLATION; BIOLOGICAL SYSTEMS; DNA; METHYLATION; TRANSCRIPTION;

BIOLOGICAL FUNCTIONS; DNA METHYLATION; EPIGENETIC MODIFICATION; GREEN FLUORESCENCE PROTEINS; GROWTH AND DEVELOPMENT; SINGLE-STRANDED DNA; SITE-SPECIFIC; TRANSCRIPTIONAL REGULATION;

POLYMERASE CHAIN REACTION;

CYCLOOXYGENASE 2; CYTOSINE; DNA; GREEN FLUORESCENT PROTEIN; SINGLE STRANDED DNA; CRE RECOMBINASE; INTEGRASE; PRIMER DNA;

AFFINITY CHROMATOGRAPHY; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; CPG ISLAND; DNA ISOLATION; DNA METHYLATION; DNA VECTOR; GENE EXPRESSION; MOUSE; NONHUMAN; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROMOTER REGION; ANIMAL; CHEMISTRY; GENETICS; METABOLISM; METHODOLOGY; NUCLEIC ACID HYBRIDIZATION; PLASMID; TUMOR CELL LINE;

ANIMALS; CELL LINE, TUMOR; CPG ISLANDS; CYCLOOXYGENASE 2; DNA METHYLATION; DNA PRIMERS; DNA, SINGLE-STRANDED; GREEN FLUORESCENT PROTEINS; INTEGRASES; MICE; NUCLEIC ACID HYBRIDIZATION; PLASMIDS; POLYMERASE CHAIN REACTION; PROMOTER REGIONS, GENETIC;

EID: 80155147107     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.05.017     Document Type: Article

References (17)

1. S.L. Berger, The complex language of chromatin regulation during transcription, Nature 447 (2007) 407-412. (Pubitemid 46816746)
2. W. Reik, Stability and flexibility of epigenetic gene regulation in mammalian development, Nature 447 (2007) 425-432. (Pubitemid 46816749)
3. E. Li, Chromatin modification and epigenetic reprogramming in mammalian development, Nat. Rev. Genet. 3 (2002) 662-673. (Pubitemid 34984255)
4. A.P. Feinberg, Phenotypic plasticity and the epigenetics of human disease, Nature 447 (2007) 433-440. (Pubitemid 46816750)
5. K. Takahashi, S. Yamanaka, Induction of pluripotent stem cells from mouse embryonic and adult fibroblast cultures by defined factors, Cell 126 (2006) 1-14.
6. A.K. Maunakea, R.P. Nagarajan, M. Bilenky, T.J. Ballinger, C. D'Souza, S.D. Fouse, B.E. Johnson, C. Hong, C. Nielsen, Y. Zhao, G. Turecki, A. Delaney, R. Varhol, N. Thiessen, K. Shchors, V.M. Heine, D.H. Rowitch, X. Xing, C. Fiore, M. Schillebeeckx, S.J.M. Jones, D. Haussler, M.A. Marra, M. Hirst, T. Wang, J.F. Costello, Conserved role of intragenic DNA methylation in regulating alternative promoters, Nature 466 (2010) 253-357.
7. Y. Guo, P. Pakneshan, J. Gladu, A. Slack, M. Szyf, S.A. Rabbani, Regulation of DNA methylation in human breast cancer, J. Biol. Chem. 277 (2002) 41571-41579.
8. M. Klug, M. Rehli, Functional analysis of promoter CpG methylation using a CpG-free luciferase reporter vector, Epigenetics 1 (2006) 127-130.
9. M. Curradi, A. Izzo, G. Badaracco, N. Landsberger, Molecular mechanism of gene silencing mediated by DNA methylation, Mol. Cell Biol. 22 (2002) 3157-3173. (Pubitemid 34437474)
10. K. Ghoshal, S. Majumder, J. Datta, T. Motiwala, S. Bai, S.M. Sharma, W. Frankel, S.T. Jacob, Role of human ribosomal RNA (rRNA) promoter methylation and of methyl-CpG-binding protein MBD2 in the suppression of rRNA gene expression, J. Biol. Chem. 279 (2004) 6783-6793. (Pubitemid 38248820)
11. A. Bacolla, S. Pradhan, R.J. Robert, R.D. Wells, Recombinant human DNA (cytosine-5) methyltrasferase, J. Biol. Chem. 274 (1999) 33011-33019.
12. A. Hermann, R. Goyal, A. Jeltsch, The Dnmt1 DNA-(cytosine-C5)- methyltransferase methylates DNA processively with high preference for hemimethylated target sites, J. Biol. Chem. 279 (2004) 48359-48450.
13. M.M. Suzuki, A. Bird, DNA methylation landscapes: provocative insights from epigenomics, Nat. Rev. Genet. 9 (2008) 465-476. (Pubitemid 351693975)
14. T.K. Wojdacz, A. Dobrovic, Melting curve assays for DNA methylation analysis, in: J. Tost (Ed.), Methods in Molecular Biology, vol. 507, Humana, New York, 2009, pp. 229-240.
15. J.D.F. Licchesi, J.G. Herman, Methylation-specific PCR, in: J. Tost (Ed.), Methods in Molecular Biology, vol. 507, Humana, New York, 2009, pp. 305-323.
16. Y.-J. Kang, B.A. Wingerd, T. Arakawa, W.L. Smith, Cyclooxygenase-2 gene transcription in a macrophage model of inflammation, J. Immunol. 177 (2006) 8111-8122. (Pubitemid 44846330)
17. M. Yossifoff, T. Kisliouk, N. Meiri, Dynamic changes in DNA methylation during thermal control establishment affect CREB binding to the brain-derived neurotrophic factor promoter, Eur. J. Neurosci. 28 (2008) 2267-2277.