Deep ultraviolet mapping of intracellular protein and nucleic acid in femtograms per pixel

Cytometry Part A

Volumn 79 A, Issue 11, 2011, Pages 920-932

  Cheung, Man C ^a   Evans, James G ^a   Mckenna, Brian ^a   Ehrlich, Daniel J ^a  

^a Boston University   (United States)

Author keywords

CHO cells; Deep ultraviolet; Hoechst; Native contrast; Nuclear stain

Indexed keywords

BENZIMIDAZOLE DERIVATIVE; FLUORESCENT DYE; HOE 33342; NUCLEIC ACID; ORGANIC COMPOUND; PROTEIN; SYTO 14; SYTOX ORANGE DYE;

ANIMAL; ARTICLE; CELL NUCLEUS; CELL ORGANELLE; CHEMISTRY; CHO CELL; CHROMATIN; CYTOCHEMISTRY; HAMSTER; METHODOLOGY; MOLECULAR IMAGING; SPECTROPHOTOMETRY; STAINING; ULTRASTRUCTURE;

ANIMALS; BENZIMIDAZOLES; CELL NUCLEUS; CHO CELLS; CHROMATIN; CRICETINAE; FLUORESCENT DYES; HISTOCYTOCHEMISTRY; MOLECULAR IMAGING; NUCLEIC ACIDS; ORGANELLES; ORGANIC CHEMICALS; PROTEINS; SPECTROPHOTOMETRY; STAINING AND LABELING;

EID: 80054924515     PISSN: 15524922     EISSN: 15524930     Source Type: Journal    
DOI: 10.1002/cyto.a.21111     Document Type: Article

References (53)

1. Zeskind BJ, Jordan CD, Timp W, Trapani L, Waller G, Horodincu V, Ehrlich DJ, Matsudaira P. Nucleic acid and protein mass mapping by live-cell deep-ultraviolet microscopy. Nat Methods 2007; 4: 567-569.
2. Gallagher SR. Quantitation of DNA and RNA with absorption and fluorescence spectroscopy. Ausubel FM, editor. Current Protocols in Molecular Biology. New York: John Wiley & Sons Inc.; 2011. p A3D.1-A3D.14.
3. Alupoaci CE, Garcia-Rubio LH. Growth behavior of microorganisms using UV-VIS spectroscopy: Escherichia coli. Biotechnol Bioeng 2004; 86: 163-167.
4. Lang-Pauluzzi I. The behaviour of the plasma membrane during plasmolysis: A study by UV microscopy. J Microsc 2000; 198: 188-198.
5. Hatfield JM, Schulze L, Ernst D. Measurement of the ultraviolet absorption in specific parts of both living and fixed mammalian cells, using a specially designed microspectrophotometer. Exp Cell Res 1970; 59: 484-486.
6. Davies HG. Ultra-violet microspectrography of living tissue culture cells. Part I. Radiation measurements. Discuss Faraday Soc 1950; 9: 442-449.
7. Doležel J, Greilhuber J. Nuclear genome size: Are we getting closer? Cytometry Part A 2010; 77A: 635-642.
8. Tiersch TR, Chandler RW, Wachtel SS, Elias S. Reference standards for flow cytometry and application in comparative studies of nuclear DNA content. Cytometry 1989; 10: 706-710.
9. Rasch EM. Feulgen-DNA cytophotometry for estimating C values. Methods Mol Biol 2004; 247: 163-201.
10. Holden MJ, Haynes RJ, Rabb SA, Satija N, Yang K, Blasic JR. Factors affecting quantitation of total DNA by UV spectroscopy and PicoGreen fluorescence. J Agric Food Chem 2009; 57: 7221-7226.
11. Steck K, El-Naggar A. Simultaneous DNA/RNA analysis of solid and hematoreticular malignancies. Methods Mol Biol 1998; 91: 167-179.
12. Crissman HA, Steinkamp JA. Rapid, simultaneous measurement of DNA, protein, and cell volume in single cells from large mammalian cell populations. J Cell Biol 1973; 59: 766-771.
13. Santistenban MS, Montmasson MP, Giroud F, Ronot X, Brugal G. Fluorescence image cytometry of nuclear DNA content versus chromatin pattern: A comparative study of ten fluorochromes. J Histochem Cytochem 1992; 40: 1789-1797.
14. Krishan A. Effect of drug efflux blockers on vital staining of cellular DNA with Hoechst 33342. Cytometry 1987; 8: 642-645.
15. Darzynkiewicz Z, Traganos F, Kapuscinski J, Staiano-Coico L, Melamed MR. Accessibility of DNA in situ to various fluorochromes: Relationship to chromatin changes during erythroid differentiation of friend leukemia cells. Cytometry 1984; 5: 355-363.
16. Martin RM, Leonhardt H, Cardoso MC. DNA labeling in living cells. Cytometry Part A 2005; 67A: 45-52.
17. Mascetti G, Carrara S, Vergani L. Relationship between chromatin compactness and dye uptake for in situ chromatin stained with DAPI. Cytometry 2001; 44: 113-119.
18. Loureiro J, Rodriguez E, Doležel, J, Santos C. Comparison of four nuclear isolation buffers for plant DNA flow cytometry. Ann Bot 2006; 98: 3679-3689.
19. Bainard JK, Fazekas AJ, Newmaster SG. Methodology significantly affects genome size estimates: Quantitative evidence using bryophytes. Cytometry Part A 2010; 77A: 725-732.
20. Klein E, Klein G. Nucleic acid content of tumour cells. Nature 1950; 166: 832-833.
21. Duck-Chong C, Pollak JK, North RJ. The relation between intracellular ribonucleic acid distribution and amino acid incorporation in the liver of the developing chick embryo. J Cell Biol 1964; 20: 25-35.
22. Bauer KD, Dethlefsen LA. Total cellular RNA content: Correlation between flow cytometry and ultraviolet spectroscopy. J Histochem Cytochem 1980; 28: 493-498.
23. DiSanto ME, Stein R, Chang S, Hypolite JA, Zheng Y, Zderic S, Wein AJ, Chacko S. Alteration in expression of myosin isoforms in detrusor smooth muscle following bladder outlet obstruction. Am J Physiol Cell Physiol 2003; 285: 1397-1410.
24. Zubkov MV, Fuchs BM, Eilers H, Burkill PH, Amann R. Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry. Appl Environ Microbiol 1999; 65: 3251-3257.
25. Aitken A, Learmonth M. Part I. Protein determination by UV absorption. Walker J, editor. The Protein Protocols Handbook. Totowa, NJ: Humana Press; 1996. p 3-6.
26. Platt JR, Klevens HB. Absolute absorption intensities of alkylbenzenes in the 2250 - 1700 Å region. Chem Rev 1947; 41: 301-310.
27. Kuipers BJ, Gruppen H. Prediction of molar extinction coefficients of proteins and peptides using UV absorption of the constituent amino acids at 214 nm to enable quantitative reverse phase high-performance liquid chromatography-mass spectrometry analysis. J Agric Food Chem 2007; 55: 5445-5451.
28. Rosenheck K, Doty P. The far ultraviolet absorption spectra of polypeptide and protein solutions and their dependence on conformation. Proc Nat Acad Sci 1961; 47: 1775-1785.
29. Tourasse, NJ, Li WH. Selective constraints, amino acid composition, and the rate of protein evolution. Mol Biol Evol 2000; 17: 656-664.
30. Saidel J, Goldfarb AR, Waldman S. The absorption spectra of amino acids in the region two hundred to two hundred and thirty millimicrons. J Biol Chem 1952; 197: 285-291.
31. Li HJ, Isenberg I, Johnson, WC. Absorption and circular dichroism studies on nucleohistone IV. Biochemistry 1971; 10: 2587-2593.
32. Tataurov AV, You Y, Owczarzy R. Predicting ultraviolet spectrum of single stranded and double stranded deoxyribonucleic acids. Biophys Chem 2008; 133: 66-70.
33. Volpe P, Eremenko-Volpe T. Quantitative studies on cell proteins in suspension cultures. Eur J Biochem 1970; 12: 195-200.
34. Crissman HA, Darzynkiewicz Z, Tobey RA, Steinkamp JA. Correlated measurements of DNA, RNA, and protein in individual cells by flow cytometry. Science 1985; 228: 1321-1324.
35. Shapiro HM. Flow Cytometric Estimation of DNA and RNA in Intact Cells Stained with Hoechst 33342 and Pyronin Y. Cytometry 1981; 2: 143-149.
36. Traganos F, Darzynkiewicz Z, Melamed MR. The ratio of RNA to total nucleic acid content as a quantitative measure of unbalanced cell growth. Cytometry 1982; 2: 212-218.
37. Krishan A, Dandekar P, Nathan N, Hamelik R, Miller C, Shaw J. DNA index, genome size, and electronic nuclear volume of vertebrates from the Miami Metro Zoo. Cytometry Part A 2005; 65A: 26-34.
38. Animal genome size database. Available at: Last accessed: October 1, 2010.
39. Greilhuber J, Volleth M, Loidl J. Genome size of man and animals relative to the plant Allium cepa. Can J Genet Cytol 1983; 25: 554-560.
40. Kato H, Harada M, Tsuchiya K, Moriwaki K. Absence of correlation between DNA repair in ultraviolet irradiated mammalian cells and life span of the donor species. Jpn J Genet 1980; 5: 99-108.
41. Bachmann K. Genome size in mammals. Chromosoma 1972; 35: 85-93
42. Darzynkiewicz Z, Halicka HD, Zhao H. Analysis of cellular DNA content by flow and laser scanning cytometry. Adv Exp Med Biol 2010; 676: 137-147.
43. Walker, PMB, Davies HG. Ultra-violet microspectrography of living tissue culture cells. Part II. Microspectrographic studies of living cells and ultraviolet-irradiated chick fibroblasts. Discuss Faraday Soc 1950; 9: 461-470.
44. Liu P-F, Avramova LV, Park C. Revisiting absorbance at 230 nm as a protein unfolding probe. Anal Biochem 2009; 389: 165-170.
45. Glazer AN, Smith EL. Studies on the ultraviolet difference spectra of proteins and polypeptides. J Biol Chem 1961; 236: 2942-2947.
46. Glazer AN, Smith EL. Effect of denaturation on the ultraviolet absorption spectra of proteins. J Biol Chem 1960; 235: PC43-PC44.
47. Martin CJ, Bhatnagar GM. Unfolding reactions of proteins. I. Kinetic and equilibrium measurements of diisopropylphosphorylchymotrypsin and chymotrypsinogen in urea. Biochemistry 1966; 5: 1230-1241.
48. Eisenberg DS, Edsall JT. Spectrophotometric titrations of human serum albumin and reduced carboxylmethylated albumin. Science 1963; 142: 50-51.
49. Mora S, Elodi P. Investigation of the near and far ultraviolet denaturation difference spectra of dehydrogenases. Eur J Biochem 1968; 5: 574-582.
50. Donovan JW. Changes in ultraviolet absorption produced by alteration of protein conformation. J Biol Chem 1969; 244: 1961-1967.
51. Borgdorff V, Pauw B, van Hees-Stuivenberg S, de Wind N. DNA mismatch repair mediates protection from mutagenesis induced by short-wave ultraviolet light. DNA Repair 2006; 5: 1364-1372.
52. Durand RE, Olive PL. Cytotoxicity, mutagenicity, and DNA damage by Hoechst 33342. J Histochem Cytochem 1982; 30: 111-116.
53. Darzynkiewicz Z, Traganos F, Kapuscinski J, Melamed MR. Denaturation and condensation of DNA in situ induced by acridine orange in relation to chromatin changes during growth and differentiation of friend erythoroleukemia cells. Cytometry 1985; 6: 195-207.