Determination of total protein content of bacterial cells by SYPRO staining and flow cytometry

Applied and Environmental Microbiology

Volumn 65, Issue 7, 1999, Pages 3251-3257

  Zubkov, Mikhail V ^a,b   Fuchs, Bernhard M ^b   Eilers, Heike ^b   Burkill, Peter H ^a   Amann, Rudolf ^b  

^a PLYMOUTH MARINE LABORATORY   (United Kingdom)

^b MAX PLANCK INSTITUTE FOR MARINE MICROBIOLOGY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

SEA WATER;

ARCOBACTER; ARTICLE; BACTERIUM; BIOMASS; FLEXIBACTERACEAE; FLOW CYTOMETRY; FLUORESCENCE; NONHUMAN; PLANKTON; PROTEIN CONTENT; PROTEIN METABOLISM; SPIRILLUM; VIBRIO;

ARCOBACTER; ARCOBACTER SP. SOLAR LAKE; BACTERIA (MICROORGANISMS); CYTOPHAGA; CYTOPHAGA SP.; FLEXIBACTERACEAE; OCEANOSPIRILLUM; PSEUDOALTEROMONAS; PSEUDOALTEROMONAS SP.; SPIRILLUM; VIBRIO; VIBRIO SP.;

EID: 0033050650     PISSN: 00992240     EISSN: None     Source Type: Journal    
DOI: 10.1128/aem.65.7.3251-3257.1999     Document Type: Article

References (35)

1. Åkerlund, T., K. Nordström, and R. Bernander. 1995. Analysis of cell size and DNA content in exponentially growing and stationary-phase batch cultures of Escherichia coli. J. Bacteriol. 177:6791-6797.
2. Allman, R., A. C. Hann, A. P. Phillips, K. L. Martin, and D. Lloyd. 1990. Growth of Azotobacter vinelandii with correlation of Coulter cell size, flow cytometric parameters and ultrastructure. Cytometry 11:822-831.
3. Bratbak, G. 1985. Bacterial biovolume and biomass estimations. Appl. Environ. Microbiol. 49:1488-1493.
4. Burkill, P. H., R. J. G. Leakey, N. J. P. Owens, and R. F. C. Mantoura. 1993. Synechococcus and its importance to the microbial foodweb of the northwestern Indian Ocean. Deep-Sea Res. Part II Top. Stud. Oceanogr. 40:773-782.
5. Christian, J. R., and D. M. Karl. 1994. Microbial community structure at the US.-Joint Global Ocean Flux Study station ALOHA: inverse methods for estimating biochemical indicator ratios. J. Geophys. Res. 99:14,269-14,276.
6. Davey, H. M., C. L. Davey, and D. B. Kell. 1993. On the determination of the size of microbial cells using flow cytometry, p. 49-65. In D. Lloyd (ed.), Flow cytometry in microbiology. Springer-Verlag, New York, N.Y.
7. DeLeo, P. C., and P. Baveye. 1996. Enumeration and biomass estimation of bacteria in aquifer microcosm studies by flow cytometry. Appl. Environ. Microbiol. 62:4580-4586.
8. Fagerbakke, K. M., M. Heldal, and S. Norland. 1996. Content of carbon, nitrogen, oxygen, sulfur and phosphorus in native aquatic and cultured bacteria. Aquat. Microb. Ecol. 10:15-27.
9. Fox, C. H., F. B. Johnson, J. Whiting, and P. P. Roller. 1985. Formaldehyde fixation. J. Histochem. Cytochem. 33:845-853.
10. Fukuda, R., H. Ogawa, T. Nagata, and I. Koike. 1998. Direct determination of carbon and nitrogen contents of natural bacterial assemblages in marine environments. Appl. Environ. Microbiol. 64:3352-3358.
11. Garcia-Ochoa, G. F., V. E. Santos, and A. Alcón. 1998. Intracellular compounds quantification by means of flow cytometry in bacteria: application to xanthan production by Xanthomonas campestris. Biotechnol. Bioeng. 57:87-94.
12. Haugland, R. P. 1996. Handbook of fluorescent probes and research chemicals, 6th ed. Molecular Probes, Inc., Eugene, Oreg.
13. Hayat, M. A. 1981. Fixation, p. 1-106. In Principles and techniques of electron microscopy. Biological applications, vol. 1. University Park Press, Baltimore, Md.
14. Heldal, M. S., S. Norland, and O. Tumyr. 1985. X-ray microanalytic method for measurement of dry matter and elemental content of individual bacteria. Appl. Environ. Microbiol. 50:1251-1257.
15. Ingraham, J. L., O. Maaløe, and F. C. Neidhardt. 1983. Growth of the bacterial cell. Sinauer Associates, Sunderland, Mass.
16. Koch, A. L. 1961. Some calculations on the turbidity of mitochondria and bacteria. Biochim. Biophys. Acta 51:429-441.
17. Koch, A. L., B. R. Robertson, and D. K. Button. 1996. Deduction of the cell volume and mass from forward scatter intensity of bacteria analyzed by flow cytometry. J. Microbiol. Methods 27:49-61.
18. Lebaron, P., and F. Joux. 1994. Flow cytometric analysis of the cellular DNA content of Salmonella typhimurium and Alteromonas haloplanktis during starvation and recovery in seawater. Appl. Environ. Microbiol. 60:4345-4350.
19. Li, W. K. W., P. M. Dickie, W. G. Harrison, and B. D. Irwin. 1993. Biomass and production of bacteria and phytoplankton during the spring bloom in the western North Atlantic Ocean. Deep-Sea Res. Part II Top. Stud. Oceanogr. 40:307-327.
20. Li, W. K. W., J. F. Jellett, and P. M. Dickie. 1995. DNA distributions in planktonic bacteria stained with TOTO or TO-PRO. Limnol. Oceanogr. 40:1485-1495.
21. Marie, D., F. Partensky, S. Jacquet, and D. Vaulot. 1997. Enumeration and cell cycle analysis of natural populations of marine picoplankton by flow cytometiy using the nucleic acid stain SYBR Green I. Appl. Environ. Microbiol. 63:186-193.
22. Monger, B. C., and M. R. Landry. 1993. Flow cytometric analysis of marine bacteria with Hoechst 33342. Appl. Environ. Microbiol. 59:905-911.
23. Norland, S. M., M. Heldal, and O. Tumyr. 1987. On the relation between dry matter and volume of bacteria. Microb. Ecol. 31:95-101.
24. Robertson, B. R., and D. K. Button. 1989. Characterizing aquatic bacteria according to population, cell size and apparent DNA content by flow cytometry. Cytometry 10:70-76.
25. Robertson, B. R., D. K. Button, and A. L. Koch. 1998. Determination of the biomasses of small bacteria at low concentrations in a mixture of species with forward light scatter measurements by flow cytometry. Appl. Environ. Microbiol. 64:3900-3909.
26. Salzman, G. C., S. B. Singham, R. G. Johnston, and C. F. Bohren. 1990. Light scattering and cytometry, p. 81-107. In M. R. Melamed, T. Lindmo, and M. L. Mendelsohn (ed.), Flow cytometry and sorting, 2nd ed. John Wiley & Sons, Inc., New York, N.Y.
27. Schut, F., E. De Vries, J. C. Gottschal, B. R. Robertson, W. Harder, R. A. Prins, and D. K. Button. 1993. Isolation of typical marine bacteria by dilution culture: growth, maintenance, and characteristics of isolates under laboratory conditions. Appl. Environ. Microbiol. 59:2150-2160.
28. Shapiro, H. M. 1995. Practical flow cytometry, 3rd ed. Wiley-Liss, Inc., New York, N.Y.
29. Sieracki, M. E., P. W. Johnson, and J. M. Stiburth. 1985. Detection, enumeration, and sizing of planktonic bacteria by image-analyzed epifluorescence microscopy. Appl. Environ. Microbiol. 49:799-810.
30. Sieracki, M. E., C. L. Viles, and K. L. Webb. 1989. Algorithm to estimate cell biovolume using image analyzed microscopy. Cytometry 10:551-557.
31. Smith, P. K., R. I. Krohn, G. T. Hermanson, A. K. Mallia, F. H. Gartner, M. D. Provenzano, E. K. Fujimoto, N. M. Goeke, B. J. Olson, and D. C. Klenk. 1985. Measurement of protein using bicinchoninic acid. Anal. Biochem. 150:76-85.
32. VanDilla, M. A., R. G. Langlois, D. Pinkel, D. Yajko, and W. K. Hadley. 1983. Bacterial characterization by flow cytometry. Science 220:620-621.
33. Veldhuis, M. J. W., G. W. Kreaay, and W. W. C. Gieskes. 1993. Growth and fluorescence characteristics of ultraplankton on a northsouth transect in the eastern North Atlantic. Deep-Sea Res. Part II Top. Stud. Oceanogr. 40:609-626.
34. Veldhuis, M. J. W., T. L. Cucci, and M. E. Sieracki. 1997. Cellular DNA content of marine phytoplankton using two new fluorochromes: taxonomic and ecological implications. J. Phycol. 33:527-541.
35. Zubkov, M. V., M. A. Sleigh, G. A. Tarran, P. H. Burkill, and R. J. G. Leakey. 1998. Picoplanktonic community structure on an Atlantic transect from 50°N to 50°S. Deep-Sea Res. Part I Oceanogr. Res. Pap. 45:1339-1355.