Isothermal titration calorimetry and surface plasmon resonance allow quantifying substrate binding to different binding sites of Bacillus subtilis xylanase

Analytical Biochemistry

Volumn 420, Issue 1, 2012, Pages 90-92

  Cuyvers, Sven ^a   Dornez, Emmie ^a   Abou Hachem, Maher ^b   Svensson, Birte ^b   Hothorn, Michael ^c   Chory, Joanne ^c   Delcour, Jan A ^a   Courtin, Christophe M ^a  

^a LEUVEN FOOD SCIENCE AND NUTRITION RESEARCH CENTRE LFORCE   (Belgium)

^b TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

^c SALK INSTITUTE FOR BIOLOGICAL STUDIES   (United States)

Author keywords

Isothermal titration calorimetry; Mechanism based inhibitor; Surface plasmon resonance; Xylanase; Xylooligosaccharides

Indexed keywords

BACTERIOLOGY; BINDING ENERGY; BINDING SITES; CALORIMETERS; CALORIMETRY; ENZYMES; ISOTHERMS; PLASMONS; RESONANCE; TITRATION;

BACILLUS SUBTILIS; BINDING AFFINITIES; ISOTHERMAL TITRATION CALORIMETRY; MECHANISM-BASED; SITE DIRECTED MUTAGENESIS; SUBSTRATE BINDING; XYLANASES; XYLOOLIGOSACCHARIDES;

SURFACE PLASMON RESONANCE;

BACTERIAL ENZYME; ENZYME VARIANT; XYLAN ENDO 1,3 BETA XYLOSIDASE;

ARTICLE; BACILLUS SUBTILIS; BINDING AFFINITY; BINDING SITE; CATALYSIS; ENZYME ACTIVE SITE; ENZYME SUBSTRATE COMPLEX; ISOTHERMAL TITRATION CALORIMETRY; NONHUMAN; PRIORITY JOURNAL; SITE DIRECTED MUTAGENESIS; SURFACE PLASMON RESONANCE;

BACILLUS SUBTILIS;

EID: 80054916704     PISSN: 00032697     EISSN: 10960309     Source Type: Journal    
DOI: 10.1016/j.ab.2011.09.005     Document Type: Article

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