Imaging the spatial orientation of subunits within membrane receptors by atomic force microscopy

Methods in Molecular Biology

Volumn 736, Issue , 2011, Pages 47-60

  Carnally, Stewart M ^a   Edwardson, J Michael ^a   Barrera, Nelson P ^b  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b PONTIFICIA UNIVERSIDAD CATÓLICA DE CHILE   (Chile)

Author keywords

AFM; Membrane receptor; Molecular architecture; Structural biology; Subunit stoichiometry

Indexed keywords

MEMBRANE PROTEIN; MEMBRANE RECEPTOR;

ALGORITHM; ATOMIC FORCE MICROSCOPY; PROTEIN PURIFICATION; PROTEIN STRUCTURE; SPATIAL ORIENTATION; ARTICLE; ISOLATION AND PURIFICATION; METHODOLOGY; MOLECULAR IMAGING; ULTRASTRUCTURE;

ALGORITHMS; MEMBRANE PROTEINS; MICROSCOPY, ATOMIC FORCE; MOLECULAR IMAGING;

EID: 80054838724     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-105-5_4     Document Type: Chapter

References (15)

1. Lacapere, J. J., Pebay-Peyroula, E., Neumann, J. M., and Etchebest, C. (2007) Determining membrane protein structures: still a challenge! Trends Biochem Sci 32, 259–270.
2. Muller, D. J. (2008) AFM: a nanotool in membrane biology Biochemistry 47, 7986–7998.
3. Shahin, V., and Barrera, N. P. (2008) Providing unique insight into cell biology via atomic force microscopy Int Rev Cytol 265, 227–252.
4. Barrera, N. P., and Edwardson, J. M. (2008) The subunit arrangement and assembly of ionotropic receptors Trends Neurosci 31, 569–576.
5. Barrera, N. P., Henderson, R. M., and Edwardson, J. M. (2008) Determination of the architecture of ionotropic receptors using AFM imaging Pflugers Arch 456, 199–209.
6. Barrera, N. P., Betts, J., You, H., Henderson, R. M., Martin, I. L., Dunn, S. M., and Edwardson, J. M. (2008) Atomic force microscopy reveals the stoichiometry and subunit arrangement of the alpha4beta3delta GABA(A) receptor Mol Pharmacol 73, 960–967.
7. Barrera, N. P., Henderson, R. M., Murrell-Lagnado, R. D., and Edwardson, J. M. (2007) The stoichiometry of P2X2/6 receptor heteromers depends on relative subunit expression levels Biophys J 93, 505–512.
8. Barrera, N. P., Herbert, P., Henderson, R. M., Martin, I. L., and Edwardson, J. M. (2005) Atomic force microscopy reveals the stoichiometry and subunit arrangement of 5-HT3 receptors Proc Natl Acad Sci USA 102, 12595–12600.
9. Barrera, N. P., Ormond, S. J., Henderson, R. M., Murrell-Lagnado, R. D., and Edwardson, J. M. (2005) Atomic force microscopy imaging demonstrates that P2X2 receptors are trimers but that P2X6 receptor subunits do not oligomerize J Biol Chem 280, 10759–10765.
10. Barrera, N. P., Shaifta, Y., McFadzean, I., Ward, J. P., Henderson, R. M., and Edwardson, J. M. (2007) AFM imaging reveals the tetra-meric structure of the TRPC1 channel Biochem Biophys Res Commun 358, 1086–1090.
11. Carnally, S. M., Dev, H. S., Stewart, A. P., Barrera, N. P., Van Bemmelen, M. X., Schild, L., Henderson, R. M., and Edwardson, J. M. (2008) Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging Biochem Biophys Res Commun 372, 752–755.
12. Ormond, S. J., Barrera, N. P., Qureshi, O. S., Henderson, R. M., Edwardson, J. M., and Murrell-Lagnado, R. D. (2006) An uncharged region within the N terminus of the P2X6 receptor inhibits its assembly and exit from the endoplasmic reticulum Mol Pharmacol 69, 1692–1700.
13. Schneider, S. W., Lärmer, J., Henderson, R. M., and Oberleithner, H. (1998) Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopy Pflugers Arch 435, 362–367.
14. Barrera, N. P., Ge, H., Henderson, R. M., Fitzgerald, W. J., and Edwardson, J. M. (2008) Automated analysis of the architecture of receptors, imaged by atomic force microscopy Micron 39, 101–110.
15. Fotiadis, D., and Engel, A. (2004) High-resolution imaging of bacteriorhodopsin by atomic force microscopy Methods Mol Biol 242, 291–303.