Direct visualization of the trimeric structure of the ASIC1a channel, using AFM imaging

Biochemical and Biophysical Research Communications

Volumn 372, Issue 4, 2008, Pages 752-755

  Carnally, Stewart M ^a   Dev, Harveer S ^a   Stewart, Andrew P ^a   Barrera, Nelson P ^a   Van Bemmelen, Miguel X ^b   Schild, Laurent ^b   Henderson, Robert M ^a   Edwardson, J Michael ^a  

^a UNIVERSITY OF CAMBRIDGE   (United Kingdom)

^b UNIVERSITY OF LAUSANNE   (Switzerland)

Author keywords

Acid sensing ion channels; Atomic force microscopy; Channel assembly; Subunit stoichiometry

Indexed keywords

ACID SENSING ION CHANNEL; ACID SENSING ION CHANNEL 1A; DIMER; MICA; MONOMER; POLYMER; TRIMER;

ARTICLE; ATOMIC FORCE MICROSCOPY; CONTROLLED STUDY; EMBRYO; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN AGGREGATION; PROTEIN ANALYSIS; PROTEIN STRUCTURE;

ALUMINUM SILICATES; HUMANS; MICROSCOPY, ATOMIC FORCE; NERVE TISSUE PROTEINS; PROTEIN SUBUNITS; SODIUM CHANNELS;

EID: 45449118679     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2008.05.100     Document Type: Article

References (21)

1. Wemmie J.A., Price M.P., and Welsh M.J. Acid-sensing ion channels: advances, questions and therapeutic opportunities. Trends Neurosci. 29 (2006) 578-586
2. Firsov D., Gautschi I., Merillat A.-M., Rossier B.C., and Schild L. The heterotetrameric architecture of the epithelial sodium channel (ENaC). EMBO J. 17 (1998) 344-352
3. Gao Y., Liu S.-S., Qiu S., Cheng W., Zheng J., and Luo J.-H. Fluorescence resonance energy transfer analysis of subunit assembly of the ASIC channel. Biochem. Biophys. Res. Commun. 359 (2007) 143-150
4. Eskandari S., Snyder P.M., Kreman M., Zampighi G.A., Welsh M.J., and Wright E.M. Number of subunits comprising the epithelial sodium channel. J. Biol. Chem. 274 (1999) 27281-27286
5. Jasti J., Furukawa H., Gonzales E.B., and Gouaux E. Structure of acid-sensing ion channel 1 at 1.9 Å resolution and low pH. Nature 449 (2007) 316-323
6. +-gated channels in mouse sensory neurons. Proc. Natl. Acad. Sci. USA 99 (2002) 2338-2343
7. + channels. J. Biol. Chem. 275 (2000) 25116-25121
8. Yermolaieva O., Leonard A.S., Schnizler M.K., Abboud F.M., and Welsh M.J. Extracellular acidosis increases neuronal cell calcium by activating acid-sensing ion channel 1a. Proc. Natl. Acad. Sci. USA 101 (2004) 6752-6757
9. Neish C.S., Martin I.L., Davies M., Henderson R.M., and Edwardson J.M. Atomic force microscopy of ionotropic receptors bearing subunit-specific tags provides a method for determining receptor architecture. Nanotechnology 14 (2003) 864-872
10. A receptor. Mol. Pharm. 73 (2008) 960-967
11. 6 receptor subunits do not oligomerize. J. Biol. Chem. 280 (2005) 10759-10765
12. 6 receptor inhibits its assembly and exit from the endoplasmic reticulum. Mol. Pharm. 69 (2006) 1692-1700
13. 2/6 receptor heteromers depends on relative subunit expression levels. Biophys. J. 93 (2007) 505-512
14. 3 receptors. Proc. Natl. Acad. Sci. USA 102 (2005) 12595-12600
15. Barrera N.P., Shaifta Y., McFadzean I., Ward J.P.T., Henderson R.M., and Edwardson J.M. AFM imaging reveals the tetrameric structure of the TRPC1 channel. Biochem. Biophys. Res. Commun. 358 (2007) 1086-1090
16. Schneider S.W., Lärmer J., Henderson R.M., and Oberleithner H. Molecular weights of individual proteins correlate with molecular volumes measured by atomic force microscopy. Pflügers Arch. 435 (1998) 362-367
17. Barrera N.P., Ge H., Henderson R.M., Fitzgerald W.J., and Edwardson J.M. Automated analysis of the architecture of receptors, imaged by atomic force microscopy. Micron 39 (2008) 101-110
18. Durchschlag H., and Zipper P. Calculation of partial specific volumes and other volumetric properties of small molecules and polymers. J. Appl. Cryst. 30 (1997) 803-807
19. Grant E.H. The dielectric method of estimating protein hydration. Phys. Med. Biol. 2 (1957) 17-28
20. Meltzer R.H., Kapoor N., Qadri Y.J., Anderson S.J., Fuller C.M., and Benos D.J. Heteromeric assembly of acid-sensitive ion channel and epithelial sodium channel subunits. J. Biol. Chem. 282 (2007) 25548-25559
21. Connolly C.N., Krishek B.J., McDonald B.J., Smart T.G., and Moss S.J. Assembly and cell surface expression of heteromeric and homomeric γ-aminobutyric acid type A receptors. J. Biol. Chem. 271 (1996) 89-96