Real-Time BRET Assays to Measure G Protein/Effector Interactions

Methods in Molecular Biology

Volumn 756, Issue , 2011, Pages 245-261

  Pétrin, Darlaine ^a   Robitaille, Mélanie ^b   Hébert, Terence E ^a  

^a MCGILL UNIVERSITY   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Bioluminescence resonance energy transfer; G protein coupled receptor; G proteins; Protein protein interaction assays

Indexed keywords

ADENYLATE CYCLASE; GUANINE NUCLEOTIDE BINDING PROTEIN; GUANINE NUCLEOTIDE BINDING PROTEIN BETA SUBUNIT; GUANINE NUCLEOTIDE BINDING PROTEIN GAMMA SUBUNIT;

ANIMAL; ARTICLE; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER; CELL LINE; HUMAN; METABOLISM; METHODOLOGY; PROTEIN ANALYSIS;

ADENYLATE CYCLASE; ANIMALS; BIOLUMINESCENCE RESONANCE ENERGY TRANSFER TECHNIQUES; CELL LINE; GTP-BINDING PROTEIN BETA SUBUNITS; GTP-BINDING PROTEIN GAMMA SUBUNITS; GTP-BINDING PROTEINS; HUMANS; PROTEIN INTERACTION MAPPING;

EID: 80054763193     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-160-4_13     Document Type: Chapter

References (30)

1. Rebois, R. V., Robitaille, M., Gales, C., Dupre, D. J., Baragli, A., Trieu, P., Ethier, N., Bouvier, M., and Hebert, T. E. (2006) Heterotrimeric G proteins form stable complexes with adenylyl cyclase and Kir3.1 channels in living cells. J Cell Sci 119, 2807–18.
2. Dupre, D. J., Baragli, A., Rebois, R. V., Ethier, N., and Hebert, T. E. (2007) Signalling complexes associated with adenylyl cyclase II are assembled during their biosynthesis. Cell Signal 19, 481–9.
3. Dupre, D. J., Robitaille, M., Ethier, N., Villeneuve, L. R., Mamarbachi, A. M., and Hebert, T. E. (2006) Seven transmembrane receptor core signaling complexes are assembled prior to plasma membrane traf ficking. J Biol Chem 281, 34561–73.
4. Rebois, R. V., Warner, D. R., and Basi, N. S. (1997) Does subunit dissociation necessarily accompany the activation of all heterotrimeric G proteins? Cell Signal 9, 141–51.
5. Levitzki, A., and Klein, S. (2002) G-protein subunit dissociation is not an integral part of G-protein action. Chembiochem 3, 815–8.
6. Evanko, D. S., Thiyagarajan, M. M., Takida, S., and Wedegaertner, P. B. (2005) Loss of association between activated Gaq and Gbg disrupts receptor-dependent and receptor-independent signaling. Cell Signal 17, 1218–28.
7. Bunemann, M., Frank, M., and Lohse, M. J. (2003) Gi protein activation in intact cells involves subunit rearrangement rather than dissociation. Proc Natl Acad Sci U S A 100, 16077–82.
8. Azpiazu, I., and Gautam, N. (2004) A fluorescence resonance energy transfer-based sensor indicates that receptor access to a G protein is unrestricted in a living mammalian cell. J Biol Chem 279, 27709–18.
9. Hein, P., Frank, M., Hoffmann, C., Lohse, M. J., and Bunemann, M. (2005) Dynamics of receptor/G protein coupling in living cells. EMBO J 24, 4106–14.
10. Gales, C., Rebois, R. V., Hogue, M., Trieu, P., Breit, A., Hebert, T. E., and Bouvier, M. (2005) Real-time monitoring of receptor and G-protein interactions in living cells. Nat Methods 2, 177–84.
11. Gibson, S. K., and Gilman, A. G. (2006) Gia and Gb subunits both define selectivity of G protein activation by a2-adrenergic receptors. Proc Natl Acad Sci U S A 103, 212–7.
12. Gales, C., Van Durm, J. J., Schaak, S., Pontier, S., Percherancier, Y., Audet, M., Paris, H., and Bouvier, M. (2006) Probing the activation-promoted structural rearrangements in preassembled receptor-G protein complexes. Nat Struct Mol Biol 13, 778–86.
13. Digby, G. J., Lober, R. M., Sethi, P. R., and Lambert, N. A. (2006) Some G protein heterotrimers physically dissociate in living cells. Proc Natl Acad Sci U S A 103, 17789–94.
14. Robitaille, M., Ramakrishnan, N., Baragli, A., and Hebert, T. E. (2009) Intracellular trafficking and assembly of specific Kir3 channel/G protein complexes. Cell Signal 21, 488–501.
15. Dupre, D. J., Robitaille, M., Richer, M., Ethier, N., Mamarbachi, A. M., and Hebert, T. E. (2007) Dopamine receptor-interacting protein 78 acts as a molecular chaperone for Gg subunits before assembly with Gb. J Biol Chem 282, 13703–15.
16. Ayoub, M. A., and Pfleger, K. D. (2010) Recent advances in bioluminescence resonance energy transfer technologies to study GPCR heteromerization. Curr Opin Pharmacol 10, 44–52.
17. Pfleger, K. D. (2009) Analysis of protein– protein interactions using bioluminescence resonance energy transfer. Methods Mol Biol 574, 173–83.
18. Hebert, T. E., Gales, C., and Rebois, R. V. (2006) Detecting and imaging protein–protein interactions during G protein-mediated signal transduction in vivo and in situ by using fluorescence-based techniques. Cell Biochem Biophys 45, 85–109.
19. Pfleger, K. D., Seeber, R. M., and Eidne, K. A. (2006) Bioluminescence resonance energy transfer (BRET) for the real-time detection of protein–protein interactions. Nat Protoc 1, 337–45.
20. Pfleger, K. D., and Eidne, K. A. (2006) Illuminating insights into protein–protein interactions using bioluminescence resonance energy transfer (BRET). Nat Methods 3, 165–74.
21. Kroeger, K. M., and Eidne, K. A. (2004) Study of G-protein-coupled receptor-protein interactions by bioluminescence resonance energy transfer. Methods Mol Biol 259, 323–33.
22. Marullo, S., and Bouvier, M. (2007) Resonance energy transfer approaches in molecular pharmacology and beyond. Trends Pharmacol Sci 28, 362–5.
23. Milligan, G., and Bouvier, M. (2005) Methods to monitor the quaternary structure of G protein-coupled receptors. FEBS J 272, 2914–25.
24. Kocan, M., See, H. B., Seeber, R. M., Eidne, K. A., and Pfleger, K. D. (2008) Demonstration of improvements to the bioluminescence resonance energy transfer (BRET) technology for the monitoring of G protein-coupled receptors in live cells. J Biomol Screen 13, 888–98.
25. Wei, H., Ahn, S., Shenoy, S. K., Karnik, S. S., Hunyady, L., Luttrell, L. M., and Lefkowitz, R. J. (2003) Independent b-arrestin 2 and G protein-mediated pathways for angiotensin II activation of extracellular signal-regulated kinases 1 and 2. Proc Natl Acad Sci U S A 100, 10782–7.
26. Tohgo, A., Choy, E. W., Gesty-Palmer, D., Pierce, K. L., Laporte, S., Oakley, R. H., Caron, M. G., Lefkowitz, R. J., and Luttrell, L. M. (2003) The stability of the G protein-coupled receptor-b-arrestin interaction determines the mechanism and functional consequence of ERK activation. J Biol Chem 278, 6258–67.
27. Zidar, D. A., Violin, J. D., Whalen, E. J., and Lefkowitz, R. J. (2009) Selective engagement of G protein coupled receptor kinases (GRKs) encodes distinct functions of biased ligands. Proc Natl Acad Sci U S A 106, 9649–54.
28. Lee, M. H., El-Shewy, H. M., Luttrell, D. K., and Luttrell, L. M. (2008) Role of b-arrestin-mediated desensitization and signaling in the control of angiotensin AT1a receptor-stimulated transcription. J Biol Chem 283, 2088–97.
29. Smith, N. J., and Luttrell, L. M. (2006) Signal switching, crosstalk, and arrestin scaffolds: novel G protein-coupled receptor signaling in cardiovascular disease. Hypertension 48, 173–9.
30. Gesty-Palmer, D., Chen, M., Reiter, E., Ahn, S., Nelson, C. D., Wang, S., Eckhardt, A. E., Cowan, C. L., Spurney, R. F., Luttrell, L. M., and Lefkowitz, R. J. (2006) Distinct b-arrestin-and G protein-dependent pathways for parathyroid hormone receptor-stimulated ERK1/2 activation. J Biol Chem 281, 10856–64.