A novel high-throughput screening assay for discovery of molecules that increase cellular Tetrahydrobiopterin

Journal of Biomolecular Screening

Volumn 16, Issue 8, 2011, Pages 836-844

  Li, Li ^a   Du, Yuhong ^a   Chen, Wei ^b   Fu, Haian ^a   Harrison, David G ^b  

^a EMORY UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b VANDERBILT UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

Cardiac diseases; Cell based assays; Fluorescence methods; Protein protein interactions; Signal transduction; Transcription factors

Indexed keywords

CELL PROTEIN; GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE FEEDBACK REGULATORY PROTEIN; GUANOSINE TRIPHOSPHATE CYCLOHYDROLASE I; NITRIC OXIDE; TETRAHYDROBIOPTERIN; UNCLASSIFIED DRUG;

ARTICLE; CELL ACTIVITY; CELL ASSAY; CONTROLLED STUDY; DRUG SYNTHESIS; ENDOTHELIUM CELL; ENZYME ACTIVITY; FLUORESCENCE RESONANCE ENERGY TRANSFER; HIGH THROUGHPUT SCREENING; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN PROTEIN INTERACTION; SIGNAL NOISE RATIO; SIGNAL TO BACKGROUND RATIO; SIGNAL TRANSDUCTION; TIME RESOLVED FLUORESCENCE RESONANCE ENERGY TRANSFER;

BIOPTERIN; CELLS, CULTURED; DRUG EVALUATION, PRECLINICAL; ENDOTHELIAL CELLS; FLUORESCENCE; FLUORESCENCE RESONANCE ENERGY TRANSFER; GTP CYCLOHYDROLASE; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; NITRIC OXIDE; NITRIC OXIDE SYNTHASE; PHYCOCYANIN; PROTEIN BINDING; SMALL MOLECULE LIBRARIES;

EID: 80054757190     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057111411088     Document Type: Article

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