Specific Gene Silencing Using RNAi in Cell Culture

Methods in Molecular Biology

Volumn 793, Issue , 2011, Pages 457-477

  Yang, Chunxing ^a   Qiu, Linghua ^a   Xu, Zuoshang ^a  

^a UNIVERSITY OF MASSACHUSETTS MEDICAL SCHOOL   (United States)

Author keywords

Gene silencing; Knockdown; miRNA; RNAi; shRNA; siRNA

Indexed keywords

EUKARYOTA;

MICRORNA; SMALL INTERFERING RNA;

ANIMAL; ARTICLE; CELL STRAIN HEK293; DEGENERATIVE DISEASE; GENE SILENCING; GENETIC PROCEDURES; GENETIC TRANSFECTION; GENETICS; HELA CELL; HUMAN; MOLECULAR GENETICS; MOUSE; NORTHERN BLOTTING; NUCLEOTIDE SEQUENCE; REVERSE TRANSCRIPTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; WESTERN BLOTTING;

ANIMALS; BASE SEQUENCE; BLOTTING, NORTHERN; BLOTTING, WESTERN; GENE KNOCKDOWN TECHNIQUES; GENETIC TECHNIQUES; HEK293 CELLS; HELA CELLS; HUMANS; MICE; MICRORNAS; MOLECULAR SEQUENCE DATA; NEURODEGENERATIVE DISEASES; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION; RNA INTERFERENCE; RNA, SMALL INTERFERING; TRANSFECTION;

EID: 80054734896     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-328-8_30     Document Type: Chapter

References (12)

1. Mello, C. C., and Conte, D. (2004) Revealing the world of RNA interference. Nature. 431, 338–342.
2. Tomari, Y., and Zamore, P. D. (2005) Perspective: machines for RNAi. Genes Dev. 19, 517–529.
3. Xia, X., Zhou, H., Huang, Y., and Xu, Z. (2006) Allele-specific RNAi selectively silences mutant SOD1 and achieves significant therapeutic benefit in vivo. Neurobiol. Dis. 23, 578–586.
4. Qiu, L., Wang, H., Xia, X., Zhou, H., and Xu, Z. (2008) A construct with fluorescent indicators for conditional expression of miRNA. BMC Biotechnology. 8, 77.
5. Pei, Y., and Tuschl, T. (2006) On the art of identifying effective and specific siRNAs. Nat Meth. 3, 670–676.
6. Birmingham, A., Anderson, E., Sullivan, K., Reynolds, A., Boese, Q., Leake, D., Karpilow, J., and Khvorova, A. (2007) A protocol for designing siRNAs with high functionality and specificity. Nat. Protocols. 2, 2068–2078.
7. Hornung, V., Guenthner-Biller, M., Bourquin, C., Ablasser, A., Schlee, M., Uematsu, S., Noronha, A., Manoharan, M., Akira, S., de Fougerolles, A., Endres, S., and Hartmann, G. (2005) Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat. Med. 11, 263–270. Epub 2005 Feb 2020.
8. Judge, A. D., Sood, V., Shaw, J. R., Fang, D., McClintock, K., and MacLachlan, I. (2005) Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotech. 23, 457–462.
9. Lan, T., Putta, M. R., Wang, D., Dai, M., Yu, D., Kandimalla, E. R., and Agrawal, S. (2009) Synthetic oligoribonucleotides-containing secondary structures act as agonists of Toll-like receptors 7 and 8. Biochemical and Biophysical Research Communications. 386, 443–448.
10. Borchert, G. M., Lanier, W., and Davidson, B. L. (2006) RNA polymerase III transcribes human microRNAs. Nat Struct Mol Biol. 13, 1097–1101.
11. Zhou, H., Xia, X. G., and Xu, Z. (2005) An RNA polymerase II construct synthesizes short-hairpin RNA with a quantitative indicator and mediates highly efficient RNAi. Nucl. Acids Res. 33, e62.
12. Cashman, N. R., Durham, H. D., Blusztajn, J. K., Oda, K., Tabira, T., Shaw, I. T., Dahrouge, S., and Antel, J. P. (1992) Neuroblastoma x spinal cord (NSC) hybrid cell lines resemble developing motor neurons. Dev Dyn. 194, 209–221.