Substrate analysis of arabidopsis pp2c-type protein phosphatases

Methods in Molecular Biology

Volumn 779, Issue , 2011, Pages 149-161

  Umbrasaite, Julija ^a   Schweighofer, Alois ^b   Meskiene, Irute ^a,c  

^a UNIVERSITY OF VIENNA   (Austria)

^b MAX PLANCK INSTITUTE OF MOLECULAR PLANT PHYSIOLOGY   (Germany)

^c VILNIUS UNIVERSITY   (Lithuania)

Author keywords

ABI2, in vitro dephosphorylation; AP2C1; Arabidopsis; HAB1; loss of function phosphatase; MAPK; mitogen activated protein kinase; MPK6; PP2C; protein kinase; protein phosphatase; selectivity of substrate; specificity of protein phosphatases; specificity to substrate

Indexed keywords

CASEIN; MITOGEN ACTIVATED PROTEIN KINASE; MITOGEN ACTIVATED PROTEIN KINASE KINASE; PHOSPHOPROTEIN PHOSPHATASE; RECOMBINANT PROTEIN;

ARABIDOPSIS; ARTICLE; ENZYME ASSAY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENETICS; ISOLATION AND PURIFICATION; METABOLISM; METHODOLOGY; PHOSPHORYLATION;

ARABIDOPSIS; CASEINS; ENZYME ASSAYS; ESCHERICHIA COLI; MITOGEN-ACTIVATED PROTEIN KINASE KINASES; MITOGEN-ACTIVATED PROTEIN KINASES; PHOSPHOPROTEIN PHOSPHATASES; PHOSPHORYLATION; RECOMBINANT PROTEINS; SUBSTRATE SPECIFICITY;

ARABIDOPSIS; ARABIDOPSIS THALIANA; MEDICAGO; MEDICAGO SATIVA;

EID: 80054733904     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-264-9_8     Document Type: Chapter

References (37)

1. Martin, D. M., Miranda-Saavedra, D. and Barton, G. J. (2009) Kinomer v. 1.0: a database of systematically classified eukaryotic protein kinases. Nucleic Acids Res 37, D244–50.
2. Dardick, C., Chen, J., Richter, T. et al. (2007) The rice kinase database. A phylogenomic database for the rice kinome. Plant Physiol 143, 579–86.
3. Kerk, D., Templeton, G. and Moorhead, G. B. (2008) Evolutionary radiation pattern of novel protein phosphatases revealed by analysis of protein data from the completely sequenced genomes of humans, green algae, and higher plants. Plant Physiol 146, 351–67.
4. Moorhead, G. B., De Wever, V., Templeton, G. et al. (2009) Evolution of protein phosphatases in plants and animals. Biochem J 417, 401–9.
5. Yuan, Q., Ouyang, S., Wang, A. et al. (2005) The institute for genomic research Osa1 rice genome annotation database. Plant Physiol 138, 18–26.
6. Virshup, D. M. and Shenolikar, S. (2009) From promiscuity to precision: protein phosphatases get a makeover. Mol Cell 33, 537–45.
7. Meskiene, I., Baudouin, E., Schweighofer, A. et al. (2003) The Stress-induced protein phosphatase 2C is a negative regulator of a mitogen-activated protein kinase. J Biol Chem 278, 18945–18945.
8. Schweighofer, A., Kazanaviciute, V., Scheikl, E. et al. (2007) The PP2C-type Phosphatase AP2C1, Which Negatively Regulates MPK4 and MPK6, Modulates Innate Immunity, Jasmonic Acid and Ethylene Levels in Arabidopsis. Plant Cell 19, 2213–24.
9. Cohen, P. T. (2004) in: Protein phosphatases, Vol. 5, pp. 1–20 (D. R. A. J. Arino, Ed.) Springer-Verlag, Berlin Heidelberg.
10. Schweighofer, A. and Meskiene, I. (2008) in: Plant Growth Signalling, Vol. 10, pp. 277–97 (L. Boegre, Ed.) Springer-Verlag, Heidelberg.
11. Rogers, J. P., Beuscher, A. E. t., Flajolet, M. et al. (2006) Discovery of protein phosphatase 2C inhibitors by virtual screening. J Med Chem 49, 1658–67.
12. Klumpp, S., Selke, D. and Hermesmeier, J. (1998) Protein phosphatase type 2C active at physiological Mg2+: stimulation by unsaturated fatty acids. FEBS Lett 437, 229–32.
13. Baudouin, E., Meskiene, I. and Hirt, H. (1999) Short Communication: Unsaturated fatty acids inhibit MP2C, a protein phosphatase 2C involved in the wound-induced MAP kinase pathway regulation. Plant J 20, 343–348.
14. Schweighofer, A., Hirt, H. and Meskiene, I. (2004) Plant PP2C phosphatases: emerging functions in stress signaling. Trends Plant Sci 9, 236–43.
15. Kerk, D. (2006) Genome-Scale Discovery and Characterization of Class-Specific Protein Sequences: An Example Using the Protein Phosphatases of Arabidopsis thaliana. Methods Mol Biol 365, 347–70.
16. Ho, D. T., Bardwell, A. J., Abdollahi, M. et al. (2003) A docking site in MKK4 mediates high affinity binding to JNK MAPKs and competes with similar docking sites in JNK substrates. J Biol Chem 278, 32662–72.
17. Kiegerl, S., Cardinale, F., Siligan, C. et al. (2000) SIMKK, a mitogen-activated protein kinase (MAPK) kinase, is a specific activator of the salt stress-induced MAPK, SIMK. Plant Cell 12, 2247–58.
18. Ulm, R., Ichimura, K., Mizoguchi, T. et al. (2002) Distinct regulation of salinity and genotoxic stress responses by Arabidopsis MAP kinase phosphatase 1. EMBO J 21, 6483–93.
19. Lee, J. S. and Ellis, B. E. (2007) Arabidopsis MAPK phosphatase 2 (MKP2) positively regulates oxidative stress tolerance and inactivates the MPK3 and MPK6 MAPKs. J Biol Chem 282, 25020–9.
20. Lee, J. S., Wang, S., Sritubtim, S. et al. (2009) Arabidopsis mitogen-activated protein kinase MPK12 interacts with the MAPK phosphatase IBR5 and regulates auxin signaling. Plant J 57, 975–85.
21. Biondi, R. M. and Nebreda, A. R. (2003) Signalling specificity of Ser/Thr protein kinases through docking-site-mediated interactions. Biochem J 372, 1–13.
22. Warmka, J., Hanneman, J., Lee, J. et al. (2001) Ptc1, a type 2C Ser/Thr phosphatase, inactivates the HOG pathway by dephosphorylating the mitogen-activated protein kinase Hog1. Mol Cell Biol 21, 51–60.
23. Nguyen, A. N. and Shiozaki, K. (1999) Heat-shock-induced activation of stress MAP kinase is regulated by threonine-and tyrosine-specific phosphatases. Genes-Dev. 1999 Jul 1; 13, 1653–63.
24. Takekawa, M., Maeda, T. and Saito, H. (1998) Protein phosphatase 2Calpha inhibits the human stress-responsive p38 and JNK MAPK pathways. Embo J 17, 4744–52.
25. Takekawa, M., Adachi, M., Nakahata, A. et al. (2000) p53-inducible wip1 phosphatase mediates a negative feedback regulation of p38 MAPK-p53 signaling in response to UV radiation. Embo J 19, 6517–26.
26. Meskiene, I., Bogre, L., Glaser, W. et al. (1998) MP2C, a plant protein phosphatase 2C, functions as a negative regulator of mitogen-activated protein kinase pathways in yeast and plants. Proc Natl Acad Sci USA 95, 1938–43.
27. Baril, C., Sahmi, M., Ashton-Beaucage, D. et al. (2009) The PP2C Alphabet is a Negative Regulator of SAPK Signaling in Drosophila. Genetics 181, 567–79.
28. Baril, C. and Therrien, M. (2006) Alphabet, a Ser/Thr phosphatase of the protein phosphatase 2C family, negatively regulates RAS/ MAPK signaling in Drosophila. Dev Biol 294, 232–45.
29. Tamura, S., Toriumi, S., Saito, J. et al. (2006) PP2C family members play key roles in regulation of cell survival and apoptosis. Cancer Sci 97, 563–7.
30. Saez, A., Apostolova, N., Gonzalez-Guzman, M. et al. (2004) Gain-of-function and loss-of-function phenotypes of the protein phosphatase 2C HAB1 reveal its role as a negative regulator of abscisic acid signalling. Plant J 37, 354–69.
31. Leung, J., Merlot, S. and Giraudat, J. (1997) The Arabidopsis ABSCISIC ACID-INSENSITIVE2 (ABI2) and ABI1 genes encode homologous protein phosphatases 2C involved in abscisic acid signal transduction. Plant Cell 9, 759–71.
32. Rodriguez, P. L., Benning, G. and Grill, E. (1998) ABI2, a second protein phosphatase 2C involved in abscisic acid signal transduction in Arabidopsis. FEBS Lett 421, 185–90.
33. Schweighofer, A., Ayatollahi, Z. and Meskiene, I. (2009) Methods Mol Biol 479, 247–60.
34. Bialojan, C. and Takai, A. (1988) Inhibitory effect of a marine-sponge toxin, okadaic acid, on protein phosphatases. Specificity and kinetics. Biochem J 256, 283–90.
35. Barford, D., Das, A. K. and Egloff, M. P. (1998) The structure and mechanism of protein phosphatases: insights into catalysis and regulation. Annu Rev Biophys Biomol Struct 27, 133–64.
36. Lawson, J. E., Niu, X. D., Browning, K. S. et al. (1993) Molecular cloning and expression of the catalytic subunit of bovine pyruvate dehydroge-nase phosphatase and sequence similarity with protein phosphatase 2C. Biochemistry 32, 8987–93.
37. Bertauche, N., Leung, J., and Giraudat, J. (1996) Protein phosphatase activity of abscisic acid insensitive 1 (ABI1) protein from Arabidopsis thaliana. Eur J Biochem 241, 193–200.