Rapid and Accurate Bacterial Identification in Probiotics and Yoghurts by MALDI-TOF Mass Spectrometry

Journal of Food Science

Volumn 76, Issue 8, 2011, Pages

  Angelakis, Emmanouil ^a   Million, Matthieu ^a   Henry, Mireille ^a   Raoult, Didier ^a  

^a CNRS   (France)

Author keywords

Functional food; Lactobacillus species; MALDI TOF MS; Probiotics; Yoghurts

Indexed keywords

BACTERIAL DNA; PROBIOTIC AGENT; YOGHURT;

ARTICLE; BACTERIAL COUNT; BIFIDOBACTERIUM; CLASSIFICATION; FERMENTATION; FOOD CONTROL; FUNCTIONAL FOOD; GENETICS; ISOLATION AND PURIFICATION; LACTOBACILLUS CASEI; LACTOBACILLUS DELBRUECKII; MASS SPECTROMETRY; METHODOLOGY; MICROBIOLOGY; REAL TIME POLYMERASE CHAIN REACTION; STREPTOCOCCUS THERMOPHILUS;

BIFIDOBACTERIUM; COLONY COUNT, MICROBIAL; DNA, BACTERIAL; FERMENTATION; FOOD MICROBIOLOGY; FUNCTIONAL FOOD; LACTOBACILLUS CASEI; LACTOBACILLUS DELBRUECKII; PROBIOTICS; REAL-TIME POLYMERASE CHAIN REACTION; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; STREPTOCOCCUS THERMOPHILUS; YOGURT;

BACTERIA (MICROORGANISMS); BIFIDOBACTERIUM ANIMALIS; LACTOBACILLUS; LACTOBACILLUS CASEI; LACTOBACILLUS DELBRUECKII; LACTOBACILLUS SP.; LACTOCOCCUS LACTIS; STREPTOCOCCUS THERMOPHILUS;

EID: 80054729724     PISSN: 00221147     EISSN: 17503841     Source Type: Journal    
DOI: 10.1111/j.1750-3841.2011.02369.x     Document Type: Article

References (37)

1. Anadon A, Martinez-Larranaga MR, Aranzazu MM. 2006. Probiotics for animal nutrition in the European Union. Regulation and safety assessment. Regul Toxicol Pharmacol 45:91-5.
2. Carcopino X, Henry M, Benmoura D, Fallabregues AS, Richet H, Boubli L, Tamalet C. 2006. Determination of HPV type 16 and 18 viral load in cervical smears of women referred to colposcopy. J Med Virol 78:1131-40.
3. Chavagnat F, Haueter M, Jimeno J, Casey MG. 2002. Comparison of partial tuf gene sequences for the identification of lactobacilli. FEMS Microbiol Lett 217:177-83.
4. Claydon MA, Davey SN, Edwards-Jones V, Gordon DB. 1996. The rapid identification of intact microorganisms using mass spectrometry. Nat Biotechnol 14:1584-86.
5. De Man JC, Rogosa M, Sharpe ME. 1960. A medium for the cultivation of lactobacilli. J Appl Bacteriol 23:130-5.
6. Dellaglio F, Felis GE, Torriani S. 2002. The status of the species Lactobacillus casei (Orla-Jensen 1916) Hansen and Lessel 1971 and Lactobacillus paracasei Collins et al. 1989. Request for an opinion. Int J Syst Evol Microbiol 52:285-7.
7. Dicks LM, Du Plessis EM, Dellaglio F, Lauer E. 1996. Reclassification of Lactobacillus casei subsp. casei ATCC 393 and Lactobacillus rhamnosus ATCC 15820 as Lactobacillus zeae nom. rev., designation of ATCC 334 as the neotype of L. casei subsp. casei, and rejection of the name Lactobacillus paracasei. Int J Syst Bacteriol 46:337-40.
8. Drisko J, Bischoff B, Giles C, Adelson M, Rao RV, McCallum R. 2005. Evaluation of five probiotic products for label claims by DNA extraction and polymerase chain reaction analysis. Dig Dis Sci 50:1113-7.
9. Fasoli S, Marzotto M, Rizzotti L, Rossi F, Dellaglio F, Torriani S. 2003. Bacterial composition of commercial probiotic products as evaluated by PCR-DGGE analysis. Int J Food Microbiol 82:59-70.
10. Fedele L, Seraglia R, Battistotti B, Pinelli C, Traldi P. 1999a. Matrix-assisted laser desorption/ionization mass spectrometry for monitoring bacterial protein digestion in yogurt production. J Mass Spectrom 34:1338-45.
11. Fedele L, Seraglia R, Battistotti B, Pinelli C, Traldi P. 1999b. Qualitative characterization of bacterial strains employed in the production of yogurt by matrix-assisted laser desorption/ionization mass spectrometry. J Mass Spectrom 34:1385-8.
12. Holzapfel WH, Haberer P, Geisen R, Bjorkroth J, Schillinger U. 2001. Taxonomy and important features of probiotic microorganisms in food and nutrition. Am J Clin Nutr 73:365S-73S.
13. Huys G, Vancanneyt M, D'Haene K, Vankerckhoven V, Goossens H, Swings J. 2006. Accuracy of species identity of commercial bacterial cultures intended for probiotic or nutritional use. Res Microbiol 157:803-10.
14. Klein G, Pack A, Bonaparte C, Reuter G. 1998. Taxonomy and physiology of probiotic lactic acid bacteria. Int J Food Microbiol 41:103-25.
15. Kotzamanidis C, Kourelis A, Litopoulou-Tzanetaki E, Tzanetakis N, Yiangou M. 2010. Evaluation of adhesion capacity, cell surface traits and immunomodulatory activity of presumptive probiotic Lactobacillus strains. Int J Food Microbiol 140:154-63.
16. Liu J, Qiu B, Luo H. 2010. Fingerprinting of yogurt products by laser desorption spray post-ionization mass spectrometry. Rapid Commun Mass Spectrom 24:1365-70.
17. Marcobal A, Underwood MA, Mills DA. 2008. Rapid determination of the bacterial composition of commercial probiotic products by terminal restriction fragment length polymorphism analysis. J Pediatr Gastroenterol Nutr 46:608-11.
18. Masco L, Huys G, De BE, Temmerman R, Swings J. 2005. Culture-dependent and culture-independent qualitative analysis of probiotic products claimed to contain bifidobacteria. Int J Food Microbiol 102:221-30.
19. Menard JP, Fenollar F, Henry M, Bretelle F, Raoult D. 2008. Molecular quantification of Gardnerella vaginalis and Atopobium vaginae loads to predict bacterial vaginosis. Clin Infect Dis 47:33-43.
20. Perea VM, Hermans K, Verhoeven TL, Lebeer SE, Vanderleyden J, De Keersmaecker SC. 2007. Identification and characterization of starter lactic acid bacteria and probiotics from Columbian dairy products. J Appl Microbiol 103:666-74.
21. Raoult D. 2008. Human microbiome: take-home lesson on growth promoters? Nature 454:690-1.
22. Raoult D. 2010. The globalization of intestinal microbiota. Eur J Clin Microbiol Infect Dis
23. Reid G. 1999. The scientific basis for probiotic strains of Lactobacillus. Appl Environ Microbiol 65:3763-6.
24. Sanders ME. 2008. Probiotics: definition, sources, selection, and uses. Clin Infect Dis 46(Suppl 2):S58-61.
25. Saulnier DM, Spinler JK, Gibson GR, Versalovic J. 2009. Mechanisms of probiosis and prebiosis: considerations for enhanced functional foods. Curr Opin Biotechnol 20:135-41.
26. Saxelin M. 2008. Probiotic formulations and applications, the current probiotics market, and changes in the marketplace: a European perspective. Clin Infect Dis 46 (Suppl 2):S76-9.
27. Scharl M, Geisel S, Vavricka SR, Rogler G. 2011. Dying in yoghurt: the number of living bacteria in probiotic yoghurt decreases under exposure to room temperature. Digestion 83:13-7.
28. Schillinger U. 1999. Isolation and identification of lactobacilli from novel-type probiotic and mild yoghurts and their stability during refrigerated storage. Int J Food Microbiol 47:79-87.
29. Schillinger U, Yousif NM, Sesar L, Franz CM. 2003. Use of group-specific and RAPD-PCR analyses for rapid differentiation of Lactobacillus strains from probiotic yogurts. Curr Microbiol 47:453-6.
30. Seng P, Drancourt M, Gouriet F, La SB, Fournier PE, Rolain JM, Raoult D. 2009. Ongoing revolution in bacteriology: routine identification of bacteria by matrix-assisted laser desorption ionization time-of-flight mass spectrometry. Clin Infect Dis 49:543-51.
31. Sheu SJ, Hwang WZ, Chen HC, Chiang YC, Tsen HY. 2009. Development and use of tuf gene-based primers for the multiplex PCR detection of Lactobacillus acidophilus, Lactobacillus casei group, Lactobacillus delbrueckii, and Bifidobacterium longum in commercial dairy products. J Food Prot 72:93-100.
32. Temmerman R, Pot B, Huys G, Swings J. 2003a. Identification and antibiotic susceptibility of bacterial isolates from probiotic products. Int J Food Microbiol 81:1-10.
33. Temmerman R, Scheirlinck I, Huys G, Swings J. 2003b. Culture-independent analysis of probiotic products by denaturing gradient gel electrophoresis. Appl Environ Microbiol 69:220-6.
34. Theunissen J, Britz TJ, Torriani S, Witthuhn RC. 2005. Identification of probiotic microorganisms in South African products using PCR-based DGGE analysis. Int J Food Microbiol 98:11-21.
35. Vanderhoof JA, Young R. 2008. Probiotics in the United States. Clin Infect Dis 46 (Suppl 2):S67-72.
36. Ventura M, Canchaya C, Meylan V, Klaenhammer TR, Zink R. 2003. Analysis, characterization, and loci of the tuf genes in Lactobacillus and Bifidobacterium species and their direct application for species identification. Appl Environ Microbiol 69, 6908-22.
37. Yeung PS, Sanders ME, Kitts CL, Cano R, Tong PS. 2002. Species-specific identification of commercial probiotic strains. J Dairy Sci 85:1039-51.