RGK family G-domain:GTP analog complex structures and nucleotide-binding properties

Journal of Molecular Biology

Volumn 413, Issue 2, 2011, Pages 372-389

  Sasson, Yehezkel ^a   Navon Perry, Leehee ^a   Huppert, Dan ^a   Hirsch, Joel A ^a  

^a TEL AVIV UNIVERSITY   (Israel)

Author keywords

conformational switch; crystallography; fluorescence spectroscopy; G protein

Indexed keywords

GUANINE NUCLEOTIDE BINDING PROTEIN; GUANOSINE 5' BETA,GAMMA IMIDOPHOSPHATE; GUANOSINE DIPHOSPHATE; GUANOSINE TRIPHOSPHATE; GUANOSINE TRIPHOSPHATE DERIVATIVE; NUCLEIC ACID BINDING PROTEIN; PROTEIN GEM; PROTEIN RAD; PROTEIN REM 1; PROTEIN REM 2; PROTEIN RGK; RHO KINASE; UNCLASSIFIED DRUG; VOLTAGE GATED CALCIUM CHANNEL;

ARTICLE; BINDING KINETICS; CRYSTAL STRUCTURE; FLUORESCENCE SPECTROSCOPY; MUTAGENESIS; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN CONFORMATION; PROTEIN PROTEIN INTERACTION; THERMODYNAMICS;

MAMMALIA;

EID: 80054729017     PISSN: 00222836     EISSN: 10898638     Source Type: Journal    
DOI: 10.1016/j.jmb.2011.08.017     Document Type: Article

References (42)

1. Reynet C., and Kahn C.R. Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans Science 262 1993 1441 1444 (Pubitemid 24035065)
2. Maguire J., Santoro T., Jensen P., Siebenlist U., Yewdell J., and Kelly K. Gem: an induced, immediate early protein belonging to the Ras family Science 265 1994 241 244 (Pubitemid 24259926)
3. Finlin B.S., and Andres D.A. Rem is a new member of the Rad- and Gem/Kir Ras-related GTP-binding protein family repressed by lipopolysaccharide stimulation J. Biol. Chem. 272 1997 21982 21988 (Pubitemid 27382820)
4. Finlin B.S., Shao H., Kadono-Okuda K., Guo N., and Andres D.A. Rem2, a new member of the Rem/Rad/Gem/Kir family of Ras-related GTPases Biochem. J. 347 2000 223 231 (Pubitemid 30199062)
5. Fischer R., Wei Y., Anagli J., and Berchtold M.W. Calmodulin binds to and inhibits GTP binding of the ras-like GTPase Kir/Gem J. Biol. Chem. 271 1996 25067 25070 (Pubitemid 26337863)
6. Heo W.D., Inoue T., Park W.S., Kim M.L., Park B.O., Wandless T.J., and Meyer T. PI(3,4,5)P3 and PI(4,5)P2 lipids target proteins with polybasic clusters to the plasma membrane Science 314 2006 1458 1461 (Pubitemid 44871952)
7. Bowzard J.B., Cheng D., Peng J., and Kahn R.A. ELMOD2 is an Arl2 GTPase-activating protein that also acts on Arfs J. Biol. Chem. 282 2007 17568 17580 (Pubitemid 47100320)
8. 2+ channels in the heart Circ. Res. 101 2007 69 77 (Pubitemid 47094625)
9. 2+ channel expression at the cell surface by the small G-protein kir/Gem Nature 411 2001 701 706 (Pubitemid 32531676)
10. Ward Y., Yap S.F., Ravichandran V., Matsumura F., Ito M., Spinelli B., and Kelly K. The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway J. Cell Biol. 157 2002 291 302 (Pubitemid 34839825)
11. Finlin B.S., Crump S.M., Satin J., and Andres D.A. Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases Proc. Natl Acad. Sci. USA 100 2003 14469 14474 (Pubitemid 37499260)
12. 2+ channel activity and insulin secretion by the Rem2 GTPase J. Biol. Chem. 280 2005 41864 41871 (Pubitemid 43023155)
13. Crump S.M., Correll R.N., Schroder E.A., Lester W.C., Finlin B.S., Andres D.A., and Satin J. L-type calcium channel alpha-subunit and protein kinase inhibitors modulate Rem-mediated regulation of current Am. J. Physiol.: Heart Circ. Physiol. 291 2006 H1959 H1971
14. 2+ channel beta-subunit and the Rem GTPase J. Biol. Chem. 281 2006 23557 23566 (Pubitemid 44274130)
15. 2+ channels by RGK GTPases J. Gen. Physiol. 128 2006 605 613 (Pubitemid 44664637)
16. 2+ channel inhibition J. Biol. Chem. 282 2007 28431 28440 (Pubitemid 47606057)
17. Andres D.A., Crump S.M., Correll R.N., Satin J., and Finlin B.S. Analyses of Rem/RGK signaling and biological activity Methods Enzymol. 407 2006 484 498
18. Opatowsky Y., Sasson Y., Shaked I., Ward Y., Chomsky-Hecht O., and Litvak Y. Structure-function studies of the G-domain from human gem, a novel small G-protein FEBS Lett. 580 2006 5959 5964 (Pubitemid 44584300)
19. Splingard A., Menetrey J., Perderiset M., Cicolari J., Regazzoni K., and Hamoudi F. Biochemical and structural characterization of the gem GTPase J. Biol. Chem. 282 2007 1905 1915 (Pubitemid 47076694)
20. Yanuar A., Sakurai S., Kitano K., and Hakoshima T. Crystal structure of human Rad GTPase of the RGK-family Genes Cells 11 2006 961 968 (Pubitemid 44084030)
21. Vetter I.R., and Wittinghofer A. The guanine nucleotide-binding switch in three dimensions Science 294 2001 1299 1304 (Pubitemid 33063089)
22. Adams P.D., Afonine P.V., Bunkoczi G., Chen V.B., Davis I.W., and Echols N. PHENIX: a comprehensive Python-based system for macromolecular structure solution Acta Crystallogr., Sect. D: Biol. Crystallogr. 66 2010 213 221
23. Colicelli J. Human RAS superfamily proteins and related GTPases Sci. STKE 2004 2004 RE13
24. Berezin C., Glaser F., Rosenberg J., Paz I., Pupko T., and Fariselli P. ConSeq: the identification of functionally and structurally important residues in protein sequences Bioinformatics 20 2004 1322 1324 (Pubitemid 38807587)
25. Remmers A.E., Posner R., and Neubig R.R. Fluorescent guanine nucleotide analogs and G protein activation J. Biol. Chem. 269 1994 13771 13778 (Pubitemid 24191119)
26. Pisareva V.P., Pisarev A.V., Hellen C.U., Rodnina M.V., and Pestova T.V. Kinetic analysis of interaction of eukaryotic release factor 3 with guanine nucleotides J. Biol. Chem. 281 2006 40224 40235 (Pubitemid 46043300)
27. Feuerstein J., Goody R.S., and Webb M.R. The mechanism of guanosine nucleotide hydrolysis by p21 c-Ha-ras. The stereochemical course of the GTPase reaction J. Biol. Chem. 264 1989 6188 6190 (Pubitemid 19106690)
28. John J., Sohmen R., Feuerstein J., Linke R., Wittinghofer A., and Goody R.S. Kinetics of interaction of nucleotides with nucleotide-free H-ras p21 Biochemistry 29 1990 6058 6065 (Pubitemid 20201412)
29. John J., Rensland H., Schlichting I., Vetter I., Borasio G.D., Goody R.S., and Wittinghofer A. Kinetic and structural analysis of the Mg(2+)-binding site of the guanine nucleotide-binding protein p21H-ras J. Biol. Chem. 268 1993 923 929 (Pubitemid 23019723)
30. Spoerner M., Herrmann C., Vetter I.R., Kalbitzer H.R., and Wittinghofer A. Dynamic properties of the Ras switch I region and its importance for binding to effectors Proc. Natl Acad. Sci. USA 98 2001 4944 4949 (Pubitemid 32397062)
31. Feig L.A. Tools of the trade: use of dominant-inhibitory mutants of Ras-family GTPases Nat. Cell Biol. 1 1999 E25 E27
32. Pilz R.B., Huvar I., Scheele J.S., Van den Berghe G., and Boss G.R. A decrease in the intracellular guanosine 5′-triphosphate concentration is necessary for granulocytic differentiation of HL-60 cells, but growth cessation and differentiation are not associated with a change in the activation state of Ras, the transforming principle of HL-60 cells Cell Growth Differ. 8 1997 53 59 (Pubitemid 27030238)
33. Yamasaki K., Shirouzu M., Muto Y., Fujita-Yoshigaki J., Koide H., and Ito Y. Site-directed mutagenesis, fluorescence, and two-dimensional NMR studies on microenvironments of effector region aromatic residues of human c-Ha-Ras protein Biochemistry 33 1994 65 73 (Pubitemid 24046497)
34. - to the GDP-bound state, in the absence of GTPase-activating proteins J. Biol. Chem. 272 1997 23138 23143 (Pubitemid 27392443)
35. Eftink M.R., and Selvidge L.A. Fluorescence quenching of liver alcohol dehydrogenase by acrylamide Biochemistry 21 1982 117 125
36. Herrmann C. Ras-effector interactions: after one decade Curr. Opin. Struct. Biol. 13 2003 122 129 (Pubitemid 36170264)
37. Buhrman G., Wink G., and Mattos C. Transformation efficiency of RasQ61 mutants linked to structural features of the switch regions in the presence of Raf Structure 15 2007 1618 1629 (Pubitemid 350213406)
38. Zhu J., Reynet C., Caldwell J.S., and Kahn C.R. Characterization of Rad, a new member of Ras/GTPase superfamily, and its regulation by a unique GTPase-activating protein (GAP)-like activity J. Biol. Chem. 270 1995 4805 4812
39. John J., Schlichting I., Schiltz E., Rosch P., and Wittinghofer A. C-terminal truncation of p21 H preserves crucial kinetic and structural properties J. Biol. Chem. 264 1989 13086 13092 (Pubitemid 19194883)
40. Ahmadian M.R., Zor T., Vogt D., Kabsch W., Selinger Z., Wittinghofer A., and Scheffzek K. Guanosine triphosphatase stimulation of oncogenic Ras mutants Proc. Natl Acad. Sci. USA 96 1999 7065 7070 (Pubitemid 29275010)
41. Otwinowski Z., and Minor W. Processing of X-ray diffraction data collected in oscillation mode Methods Enzymol. 276 1997 307 326 (Pubitemid 27085611)
42. Lakowicz J.R. Principles of Fluorescence Spectroscopy 3rd edit 2006 Springer New York, NY