Identification of Mammalian Protein Complexes by Lentiviral-Based Affinity Purification and Mass Spectrometry

Methods in Molecular Biology

Volumn 781, Issue , 2011, Pages 31-45

  Ni, Zuyao ^a   Olsen, Jonathan B ^a   Emili, Andrew ^a,b   Greenblatt, Jack F ^a  

^a UNIVERSITY OF TORONTO   (Canada)

^b UNIVERSITY OF TORONTO   (Canada)

Author keywords

Affinity purification; Gateway cloning; Lentivirus; Mass spectrometry; Protein complexes; Protein protein interactions

Indexed keywords

PROTEIN; PROTEOME;

AFFINITY CHROMATOGRAPHY; ANIMAL; ARTICLE; CELL LINE; CELL STRAIN HEK293; GENETICS; HUMAN; LENTIVIRINAE; METABOLISM; METHODOLOGY; MOLECULAR CLONING; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN PROTEIN INTERACTION; PROTEOMICS; TANDEM MASS SPECTROMETRY;

ANIMALS; CELL LINE; CHROMATOGRAPHY, AFFINITY; CLONING, MOLECULAR; HEK293 CELLS; HUMANS; LENTIVIRUS; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN INTERACTION MAPS; PROTEINS; PROTEOME; PROTEOMICS; TANDEM MASS SPECTROMETRY;

LENTIVIRUS; MAMMALIA;

EID: 80054723786     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-276-2_2     Document Type: Chapter

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