메뉴 건너뛰기




Volumn 59, Issue 2, 2008, Pages 232-241

Development of a novel Gateway-based vector system for efficient, multiparallel protein expression in Escherichia coli

Author keywords

Fusion proteins; Multiparallel expression; Protein expression; Protein labeling; Recombination cloning

Indexed keywords

RECOMBINANT PROTEIN;

EID: 42749087552     PISSN: 10465928     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.pep.2008.02.003     Document Type: Article
Times cited : (19)

References (13)
  • 1
    • 33746690300 scopus 로고    scopus 로고
    • Cloning technologies for protein expression and purification
    • Hartley J.L. Cloning technologies for protein expression and purification. Current Opinion in Biotechnology 17 (2006) 359-366
    • (2006) Current Opinion in Biotechnology , vol.17 , pp. 359-366
    • Hartley, J.L.1
  • 3
    • 33846894709 scopus 로고    scopus 로고
    • Optimisation of protein expression systems for modern drug discovery
    • Forstner M., Leder L., and Mayr L.M. Optimisation of protein expression systems for modern drug discovery. Expert Review of Proteomics 4 (2007) 67-78
    • (2007) Expert Review of Proteomics , vol.4 , pp. 67-78
    • Forstner, M.1    Leder, L.2    Mayr, L.M.3
  • 4
    • 33746006525 scopus 로고    scopus 로고
    • Evaluation of two novel tag-based labelling technologies for site-specific modification of proteins
    • Tirat A., Freuler F., Stettler T., Mayr L.M., and Leder L. Evaluation of two novel tag-based labelling technologies for site-specific modification of proteins. Biological Macromolecules 39 (2006) 66-76
    • (2006) Biological Macromolecules , vol.39 , pp. 66-76
    • Tirat, A.1    Freuler, F.2    Stettler, T.3    Mayr, L.M.4    Leder, L.5
  • 5
    • 0038395853 scopus 로고    scopus 로고
    • A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process
    • Kohli B.M., and Ostermeier C. A Rubredoxin based system for screening of protein expression conditions and on-line monitoring of the purification process. Protein Expression and Purification 28 (2003) 362-367
    • (2003) Protein Expression and Purification , vol.28 , pp. 362-367
    • Kohli, B.M.1    Ostermeier, C.2
  • 6
    • 0033676098 scopus 로고    scopus 로고
    • DNA cloning using in vitro site-specific recombination
    • Hartley J.L., Temple G.F., and Brasch M.A. DNA cloning using in vitro site-specific recombination. Genome Research 10 (2000) 1788-1795
    • (2000) Genome Research , vol.10 , pp. 1788-1795
    • Hartley, J.L.1    Temple, G.F.2    Brasch, M.A.3
  • 7
    • 23044514646 scopus 로고    scopus 로고
    • Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli
    • Busso D., Delagoutte-Busso B., and Moras D. Construction of a set Gateway-based destination vectors for high-throughput cloning and expression screening in Escherichia coli. Analytical Biochemistry 343 (2005) 313-321
    • (2005) Analytical Biochemistry , vol.343 , pp. 313-321
    • Busso, D.1    Delagoutte-Busso, B.2    Moras, D.3
  • 8
    • 0023042283 scopus 로고
    • Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes
    • Studier F.W., and Moffatt B.A. Use of bacteriophage T7 RNA polymerase to direct selective high-level expression of cloned genes. Journal of Molecular Biology 189 (1986) 113-130
    • (1986) Journal of Molecular Biology , vol.189 , pp. 113-130
    • Studier, F.W.1    Moffatt, B.A.2
  • 9
    • 0031828101 scopus 로고    scopus 로고
    • Sticky-end PCR: new method for subcloning
    • Zeng G. Sticky-end PCR: new method for subcloning. Biotechniques 25 (1998) 206-208
    • (1998) Biotechniques , vol.25 , pp. 206-208
    • Zeng, G.1
  • 12
    • 15444374558 scopus 로고    scopus 로고
    • β-site specific intrabodies to decrease and prevent generation of Alzheimer's Aβ peptide
    • Paganetti P., Calanca V., Galli C., Stefani M., and Molinari M. β-site specific intrabodies to decrease and prevent generation of Alzheimer's Aβ peptide. Journal of Cell Biology 168 (2005) 863-868
    • (2005) Journal of Cell Biology , vol.168 , pp. 863-868
    • Paganetti, P.1    Calanca, V.2    Galli, C.3    Stefani, M.4    Molinari, M.5
  • 13
    • 13244265563 scopus 로고    scopus 로고
    • Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression
    • Dyson M.R., Shadbolt S.P., Vincent K.J., Perera R.L., and McCafferty J. Production of soluble mammalian proteins in Escherichia coli: identification of protein features that correlate with successful expression. BMC Biotechnology 4 (2004) 32
    • (2004) BMC Biotechnology , vol.4 , pp. 32
    • Dyson, M.R.1    Shadbolt, S.P.2    Vincent, K.J.3    Perera, R.L.4    McCafferty, J.5


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.