Three-dimensional culture of rabbit nucleus pulposus cells in collagen microspheres

Spine Journal

Volumn 11, Issue 10, 2011, Pages 947-960

  Yuan, Minting ^a   Leong, Kam W ^b   Chan, Barbara P ^a  

^a UNIVERSITY OF HONG KONG   (Hong Kong)

^b Duke University   (United States)

Author keywords

3D culture; Collagen; Intervertebral disc; Microencapsulation; Nucleus pulposus cells

Indexed keywords

CELL MARKER; COLLAGEN; COLLAGEN TYPE 1; COLLAGEN TYPE 2; CYTOKERATIN 19; GLYCOSAMINOGLYCAN; MICROSPHERE;

ANIMAL CELL; ARTICLE; CELL DENSITY; CELL ENCAPSULATION; CELL GROWTH; CELL ISOLATION; CELL KINETICS; CELL STRUCTURE; CELL ULTRASTRUCTURE; CELL VIABILITY; CONTROLLED STUDY; CULTURE TECHNIQUE; MONOLAYER CULTURE; NONHUMAN; NUCLEUS PULPOSUS; PHENOTYPE; PRIORITY JOURNAL; PROTEIN EXPRESSION; RABBIT; THREE DIMENSIONAL CULTURE;

ANIMALS; CELL CULTURE TECHNIQUES; CELLS, CULTURED; COLLAGEN; EXTRACELLULAR MATRIX; INTERVERTEBRAL DISC; MICROSPHERES; RABBITS;

EID: 80054091470     PISSN: 15299430     EISSN: 18781632     Source Type: Journal    
DOI: 10.1016/j.spinee.2011.07.004     Document Type: Article

References (49)

1. N.J. Manek, and A.J. MacGregor Epidemiology of back disorders: prevalence, risk factors, and prognosis Curr Opin Rheumatol 17 2005 134 140 (Pubitemid 40279606)
2. A.J. Freemont, A. Watkins, and C. Le Maitre Current understanding of cellular and molecular events in intervertebral disc degeneration: implications for therapy J Pathol 196 2002 374 379 (Pubitemid 34262847)
3. J.P.G. Urban, and S. Roberts Degeneration of intervertebral disc Arthritis Res Ther 5 2003 120 130
4. J.S. Silber, D.G. Anderson, V.M. Hayes, and A.R. Vaccaro Advances in surgical management of lumbar degenerative disease Orthopedics 25 2002 767 771 (Pubitemid 36395010)
5. P. Enker, A. Steffee, and C. Mcmillin Artificial disc replacement: preliminary report with a 3-year minimum follow-up Spine 18 1993 1061 1070 (Pubitemid 23201495)
6. ® SB Charité intervertebral prosthesis: the initial European Experience Spine 19 1994 1842 1849 (Pubitemid 24314197)
7. S.S. Sivan, E. Tsitron, and E. Wachtel Aggrecan turnover in human intervertebral disc as determined by the racemization of aspartic acid J Biol Chem 281 2006 13009 13014 (Pubitemid 43855209)
8. C.D. Sanchez-Bustamante, J.M. Kelm, B. Mitta, and M. Fussenegger Heterologous protein production capacity of mammalian cells cultivated as monolayers and microtissues Biotechnol Bioeng 93 2005 169 180
9. W.E. Johnson, A. Wootton, and A. El Haj Topographical guidance of intervertebral disc cell growth in vitro: towards the development of tissue repair strategies for annulus fibrosus Eur Spine J 15 Suppl 3 2006 S389 S396 (Pubitemid 44371018)
10. E. Zamir, and B. Geiger Molecular complexity and dynamics of cell-matrix adhesions J Cell Sci 114 2001 3583 3590 (Pubitemid 33041040)
11. I.V. Yannas Natural materials B.D. Ratner, A.S. Hoffman, F.J. Schoen, J.E. Lemons, Biomaterials sciences - an introduction to materials in medicine 1996 Academic Press San Diego, CA 84 93
12. A.K. Lynn, I.V. Yannas, and W. Bonfield Antigenicity and immunogenicity of collagen J Biomed Mater Res B Appl Biomater 71 2004 343 354 (Pubitemid 39463770)
13. B.P. Chan, T.Y. Hui, and C.W. Yueng Self-assembled collagen-human mesenchymal stem cell microspheres for regenerative medicine Biomaterials 28 2007 4652 4666 (Pubitemid 47260704)
14. T.Y. Hui, K.M. Cheung, and W.L. Cheung In vitro chondrogenic differentiation of human mesenchymal stem cells in collagen microspheres: influence of cell seeding density and collagen concentration Biomaterials 29 2008 3201 3212
15. B.P. Chan, T.Y. Hui, and M.Y. Wong Mesenchymal stem cell-encapsulated collagen microspheres for bone tissue engineering Tissue Eng Part C Methods 16 2010 225 235
16. C.W. Yeung, K. Cheah, D. Chan, and B.P. Chan Effects of reconstituted collagen matrix on fates of mouse embryonic stem cells before and after induction for chondrogenic differentiation Tissue Eng Part A 15 2009 3071 3085
17. H.W. Cheng, Y.K. Tsui, and K.M. Cheung Decellularization of chondrocyte-encapsulated collagen microspheres: a three-dimensional model to study the effects of acellular matrix on stem cell fate Tissue Eng Part C Methods 15 2009 697 706
18. H.L. Wong, M.X. Wang, and P.T. Cheung A 3D collagen microsphere culture system for GDNF-secreting HEK293 cells with enhanced protein productivity Biomaterials 28 2007 5369 5380
19. M. Lee, A.C. Lo, and P.T. Cheung Drug carrier systems based on collagen-alginate composite structures for improving the performance of GDNF-secreting HEK293 cells Biomaterials 30 2009 1214 1221
20. C.H. Li, T.K. Chik, and A.H.W. Ngan Correlation between compositional and mechanical properties of human mesenchymal stem cell-collagen microspheres during chondrogenic differentiation Tissue Eng Part A 17 2011 777 788
21. I. Barbosa, S. Garcia, and V. Barbier-Chassefire Improved and simple micro assay for sulfated glycosaminoglycans quantification in biological extracts and its use in skin and muscle tissue studies Glycobiology 13 2003 647 653 (Pubitemid 37220443)
22. Y.J. Kim, R.L.Y. Sah, J.-Y.H. Doong, and A.J. Grodzinsky Fluorometric assay of DNA in cartilage explants using Hoechst 33258 Anal Biochem 174 1988 168 176 (Pubitemid 18261664)
23. A. Maroudas, R.A. Stockwell, A. Nachemson, and J. Urban Factors involved in the nutrition of the human lumbar intervertebral disc: cellularity and diffusion of glucose in vitro J Anat 120 1975 113 130
24. S.M. Richardson, R.V. Walker, and S. Parker Intervertebral disc cell-mediated mesenchymal stem cell differentiation Stem Cells 24 2006 707 716 (Pubitemid 44464775)
25. B.A. Maldonado, and T.R. Oegema Initial characterization of the metabolism of intervertebral disc cells encapsulated in microspheres J Orthop Res 10 1992 677 690
26. G. Sowa, G. Vadal, and J. Kang Characterization of intervertebral disc aging - longitudinal analysis of a rabbit model by magnetic resonance imaging, histology, and gene expression Spine 33 2008 1821 1828
27. H.E. Gruber, K. Leslie, and J. Ingram Cell-based tissue engineering for the intervertebral disc: in vitro studies of human disc cell gene expression and matrix production within selected cell carriers Spine J 4 2004 44 55 (Pubitemid 38100900)
28. J.Y. Wang, A.E. Baer, V.B. Kraus, and L.A. Setton Intervertebral disc cells exhibit differences in gene expression in alginate and monolayer culture Spine 26 2001 1747 1751
29. H.A. Horner, S. Roberts, and R.C. Bielby Cells from different regions of the intervertebral disc: effect of culture system on matrix expression and cell phenotype Spine 27 2002 1018 1028 (Pubitemid 34527146)
30. P. Gaetani, M.L. Torre, and M. Klinger Adipose-derived stem cell therapy for intervertebral disc regeneration: an in vitro reconstructed tissue in alginate capsules Tissue Eng Part A 14 2008 1415 1423
31. A.I. Chou, A.T. Reza, and S.B. Nicoll Distinct intervertebral disc cell populations adopt similar phenotypes in three-dimensional culture Tissue Eng Part A 14 2008 2079 2087
32. C.L. Korecki, C.K. Kuo, R.S. Tuan, and J.C. Iatridis Intervertebral disc cell response to dynamic compression is age and frequency dependent J Orthop Res 27 2009 800 806
33. P.D. Benya, and J.D. Shaffer Dedifferentiated chondrocytes reexpress the differentiated collagen phenotype when cultured in agarose gels Cell 30 1982 215 224 (Pubitemid 13237099)
34. H.E. Gruber, G.L. Hoelscher, and K. Leslie Three-dimensional culture of human disc cells within agarose or a collagen sponge: assessment of proteoglycan production Biomaterials 27 2006 371 376 (Pubitemid 41457240)
35. H.E. Gruber, G. Hoelscher, J.A. Ingram, and E.N. Hanley Jr. Culture of human anulus fibrosus cells on polyamide nanofibers: extracellular matrix production Spine 34 2008 4 9
36. M. Sha'ban, S.J. Yoon, and Y.K. Ko Fibrin promotes proliferation and matrix production of intervertebral disc cells cultured in three-dimensional poly(lactic-co-glycolic acid) scaffold J Biomater Sci Polym Ed 19 2008 1219 1237
37. P. Grohn, G. Klock, and U. Zimmermann Collagen-coated Ba2+-alginate microcarriers for the culture of anchorage-dependent mammalian cells Biotechniques 22 1997 970 975 (Pubitemid 27212718)
38. J. Guo, G.W. Jourdian, and D.K. MacCallum Culture and growth characteristics of chondrocytes encapsulated in alginate beads Connect Tissue Res 19 1989 277 297
39. B.P. Chan, and K.W. Leong Scaffolding in tissue engineering: general approaches and tissue-specific considerations Eur Spine J 17 Suppl 4 2008 467 479
40. J.C. Iatridis, M. Weidenbaum, L.A. Setton, and V.C. Mow Is the nucleus pulposus a solid or a fluid? Mechanical behaviors of the nucleus pulposus of the human intervertebral disc Spine 21 1996 1174 1184 (Pubitemid 26161382)
41. P.J. Roughley Articular cartilage and changes in arthritis noncollagenous proteins and proteoglycans in the extracellular matrix of cartilage Arthritis Res 3 2001 342 347 (Pubitemid 33077114)
42. C.R. Lee, D. Sakai, and T. Nakai A phenotypic comparison of intervertebral disc and articular cartilage cells in the rat Eur Spine J 16 2007 2174 2185 (Pubitemid 350276086)
43. J. Clouet, G. Grimandi, and M. Pot-Vaucel Identification of phenotypic discriminating markers for intervertebral disc cells and articular chondrocytes Rheumatology (Oxford) 48 2009 1447 1450
44. Y. Cui, J. Yu, J.P.G. Urban, and D.A. Young Differential gene expression profiling of metalloproteinases and their inhibitors: a comparison between bovine intervertebral disc nucleus pulposus cells and articular chondrocytes Spine 35 2010 1101 1108
45. J. Chen, L. Jing, and C.L. Gilchrist Expression of laminin isoforms, receptors, and binding proteins unique to nucleus pulposus cells of immature intervertebral disc Connect Tissue Res 50 2009 294 306
46. P. Stosiek, M. Kasper, and U. Karsten Expression of cytokeratin and vimentin in nucleus pulposus cells Differentiation 39 1988 78 81 (Pubitemid 19045796)
47. C. Viebahn, E.B. Lane, and F.C. Ramaekers Keratin and vimentin expression in early organogenesis of the rabbit embryo Cell Tissue Res 253 1988 553 562
48. V. Barak, H. Goike, K.W. Panaretakis, and R. Einarsson Clinical utility of cytokeratins as tumor markers Clin Biochem 37 2004 529 540 (Pubitemid 38845016)
49. D. Sakai, T. Nakai, and J. Mochida Differential phenotype of intervertebral disc cells: microarray and immunohistochemical analysis of canine nucleus pulposus and anulus fibrosus Spine 34 2009 1448 1456