Measuring stall forces in vivo with optical tweezers through light momentum changes

Proceedings of SPIE - The International Society for Optical Engineering

Volumn 8097, Issue , 2011, Pages

  Mas, J ^a   Farre A ^a   Lopez Quesada C ^a   Fernandez X ^a   Martin Badosa E ^a   Montes Usategui, M ^a  

^a UNIVERSITY OF BARCELONA   (Spain)

Author keywords

Force measurements; In vivo; Light momentum; Molecular motors; Myosin; Optical tweezers; Stall force

Indexed keywords

ALLIUM CEPA; BIOLOGICAL PROCESS; DIRECT MEASUREMENT; FAST DYNAMICS; FORCE CALIBRATION; IN VIVO; IN-VITRO; IN-VIVO EXPERIMENTS; LIVING CELL; MOLECULAR MOTORS; MYOSIN; NATURAL ENVIRONMENTS; SINGLE BEAM; STALL FORCE;

LINEAR MOTORS; MICROMANIPULATORS;

OPTICAL TWEEZERS;

EID: 80054086320     PISSN: 0277786X     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.893135     Document Type: Conference Paper

References (24)

1. Ashkin, A., "Optical trapping and manipulation of neutral particles using lasers," Proc. Nat. Acad. Sci. 94(10), 4853-4860 (1997).
2. Visscher, K., Gross, S. P. and Block, S. M., "Construction of multiple-beam optical traps with nanometer resolution position sensing," IEEE J. Sel. Top. Quantum Electron. 2(4), 1066-1076 (1996).
3. Simmons, R. M., Finer, J. T., Chu, S., Spudich, J. A., "Quantitative measurements of force and displacement using an optical trap," Biophys. J. 70(4), 1813-1822 (1996).
4. Veigel, C. and Schmidt, C. F., "Moving into the cell: single-molecule studies of molecular motors in complex environments," Nat. Rev. Mol. Cell. Biol. 12, 163-176 (2011).
5. Gross, S. P., "Application of optical traps in vivo", Meth. Enzymol. 361, 162-174 (2003).
6. Dufrene, Y.f., Evans, E., Engel, A., Helenius, J., Gaub, H. E. and Müller, "Five challenges to bringing singlemolecule force spectroscopy into living cells," Nat. Meth. 8, 123-127 (2011).
7. Berg-Sørensen, K. and Flyvbjerg, H., "Power spectrum analysis for optical tweezers," Rev. Sci. Instrum. 75(3), 594-612 (2004).
8. Fischer, M., Richardson, A. C., Reihani, S. N. S., Oddershede, L. B. and Berg-Sørensen, K., "Active-passive calibration of optical tweezers in viscoelastic media," Rev. Sci. Instrum. 81, 015103 (2010).
9. Sims, P. A. and Xie, X. S., "Probing dynein and kinesin stepping with mechanical manipulation in a living cell," ChemPhysChem 10, 1511-1516 (2009).
10. Smith, S. B., Cui, Y. and Bustamante, C., "Optical-trap force transducer that operates by direct measurement of light momentum," Meth. Enzymol. 361, 134-162 (2003).
11. Farré, A. and Montes-Usategui, M. "A force detection technique for single-beam optical traps based on direct measurement of light momentum changes," Opt. Express 18(11), 11955-11968 (2010).
12. Liesener, J., Reicherter, M., Haist, T. and Tiziani, H., "Multi-functional optical tweezers using computergenerated holograms," Opt. Commun. 185(1), 77-82 (2000).
13. Farré, A., López-Quesada, C., Andilla, J., Martín-Badosa, E. and Montes-Usategui, M., "Holographic optical manipulation of motor-driven membranous structures in living NG-108 cells," Opt. Eng. 49(8), 085801 (2010).
14. Gittes, F. and Schmidt, C. F., "Interference model for back-focal-plane displacement detection in optical tweezers," Optic. Lett. 23(1), 7-9 (1998).
15. Farré, A., Montes-Usategui, M., "Back-focal-plane interferometry. Position or force detection?," Proc. SPIE, this same volume (2011).
16. http://cismm.cs.unc.edu/downloads?dl-cat=3
17. López-Quesada, C., Joseph, M., Selva, J., Farré, A., Egea, G., Ludevid, M. D., Martín-Badosa, E. and Montes-Usategui, M., "High speed tracking of intracellular structures: understanding the transport mechanisms i7n living plant cells", Proc. SPIE, this same volume (2011)
18. Kerssemakers, J. W. J., Munteanu, E. L., Laan, L., Noetzel, T. L., Janson, M. E. and Dogterom, M., "Assembly dynamics of microtubules at molecular resolution", Nature 442, 709-712 (2006).
19. Tominaga, M., Kojima, H., Yokota, E., Orii, H., Nakamori, R., Katayama, E., Anson, M., Shimmen, T. and Oiwa, K., "Higher plant myosin XI moves processively on actin with 35 nm steps at high velocity," EMBO J. 22, 1263-1272 (2003).
20. Shimmen, T. and Yokota, E. "Cytoplasmic streaming in plants," Curr. Opin. Cell. Biol. 16(1), 68-72 (2004).
21. Allersma, M. W., Gittes, F., deCastro, M. J., Stewart, R. J. and Schmidt, C.F., "Two-dimensional tracking of ncd motility by back focal plane interferometry", Biophys. J. 74(2), 1074-1085 (1998).
22. Shtridelman, Y. Cahyuti, T., Townsend, B., DeWitt, D. and Macosko, J. C., "Force-velocity curves of motor proteins cooperating in vivo," Cell Biochem. Biophys. 52(1), 19-29 (2008).
23. Kimura, Y., Toyoshima, N., Hirakawa, N., Okamoto, K. and Ishijima, A., "A kinetic mechanism for the fast movement of chara myosin," J. Mol. Biol. 328(4), 939-950 (2003).
24. Gagliano, J., Walb, M., Blaker, B., Macosko, J. C. and Holzwarth, G., "Kinesin velocity increases with the number of motors pulling against viscoelastic drag," Eur. Biophys. J. 39(5), 801-813 (2010).