One-step protein purification: Use of a novel epitope tag for highly efficient detection and purification of recombinant proteins

Open Biotechnology Journal

Volumn 5, Issue 1, 2011, Pages 1-6

  Rasche, Stefan ^a   Martin, Alexander ^b   Holzem, Achim ^c   Fischer, Rainer ^a,c   Schinkel, Helga ^a   Schillberg, Stefan ^a  

^a FRAUNHOFER INSTITUTE FOR MOLECULAR BIOLOGY AND APPLIED ECOLOGY IME   (Germany)

^b AACHEN UNIVERSITY OF APPLIED SCIENCES   (Germany)

^c RWTH AACHEN UNIVERSITY   (Germany)

Author keywords

Epitope tag; Immunoaffinity; Protein purification; Tag54; Tobacco

Indexed keywords

C-TERMINUS; CHLORAMPHENICOL ACETYLTRANSFERASE; EFFICIENT DETECTION; EPITOPE TAG; HIGH SENSITIVITY; IMMUNOAFFINITY; IMMUNOAFFINITY CHROMATOGRAPHY; IMMUNOBLOTS; PROTEIN PURIFICATION; RECOMBINANT PROTEIN; TAG54; TAGGED PROTEINS; TOBACCO MOSAIC VIRUS;

AFFINITY CHROMATOGRAPHY; ANTIGEN-ANTIBODY REACTIONS; ANTIGENS; CHEMICAL DETECTION; COMPUTER VIRUSES; GENES; MONOCLONAL ANTIBODIES; PLANT EXTRACTS; PURIFICATION; TOBACCO; VIRUSES;

PROTEINS;

CHLORAMPHENICOL ACETYLTRANSFERASE; EPITOPE; PLANT EXTRACT; RECOMBINANT PROTEIN; RNA DIRECTED RNA POLYMERASE;

ARTICLE; BINDING AFFINITY; CARBOXY TERMINAL SEQUENCE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; EPITOPE MAPPING; MOLECULAR LIBRARY; MOUSE; NONHUMAN; NUCLEOTIDE SEQUENCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN BINDING; PROTEIN DOMAIN; PROTEIN EXPRESSION; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; SEQUENCE ANALYSIS; TOBACCO MOSAIC VIRUS;

NICOTIANA TABACUM; TOBACCO MOSAIC VIRUS;

EID: 80053545207     PISSN: None     EISSN: 18740707     Source Type: Journal    
DOI: 10.2174/1874070701105010001     Document Type: Article

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