Multiple tandem epitope tagging for enhanced detection of protein expressed in mammalian cells

Applied Biochemistry and Biotechnology - Part B Molecular Biotechnology

Volumn 19, Issue 3, 2001, Pages 313-321

  Zhang, L ^a   Hernan, R ^a   Brizzard, B ^a  

^a Sigma Chemical Company   (United States)

Author keywords

Mammalian cells; Recombinant protein; Triple FLAG epitope

Indexed keywords

EPITOPE; HYBRID PROTEIN;

ACCURACY; ANIMAL CELL; ARTICLE; CONTROLLED STUDY; IMMUNOHISTOCHEMISTRY; IMMUNOPRECIPITATION; MAMMAL CELL; NONHUMAN; PROTEIN ANALYSIS; PROTEIN EXPRESSION; WESTERN BLOTTING;

ANIMALS; BLOTTING, WESTERN; COS CELLS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; EPITOPE MAPPING; IMMUNOHISTOCHEMISTRY; PLASMIDS; RECOMBINANT PROTEINS; SENSITIVITY AND SPECIFICITY;

EID: 0035173204     PISSN: 10736085     EISSN: None     Source Type: Journal    
DOI: 10.1385/MB:19:3:313     Document Type: Article

References (8)

1. A C-terminal signal pevents secretion of luminal ER proteins
2. Purification of an RAS-responsive adenylyl cyclase complex from Saccharomyces cerevisiae by use of an epitope addition method
3. Epitope tagging and protein surveillance
4. The tails of ubiquitin precursors are ribosomal proteins whose fusion to ugiquirin facilitates ribosome biogenesis
5. A short polypeptide maker sequence useful for recombinant protein identification and purification
6. Immunoaffinity purification of FLAG epitope-tagged bacterial alkaline phosphatase using a novel monoclonal antibody and peptide elution
7. Identification of new tag sequences with differential and selective recognition properties for the anti-FLAG monoclonal antibosdies M1, M2, and M5
8. Multiple epitope tagging of expressed proteins for enhanced detection