Cellular assay optimization: Part I: The use of large-scale transiently transfected cryobanks and introduction of a c-Myc tag to design a standardized ELISA process

Journal of Biomolecular Screening

Volumn 16, Issue 9, 2011, Pages 959-966

  MacMillan, Marie A ^a   Fisher, David I ^a   Roberts, Karen ^a   Orme, Jonathan P ^a  

^a ASTRAZENECA   (United Kingdom)

Author keywords

c Myc; cryopreservation; ELISA; kinase; transient transfections

Indexed keywords

MYC PROTEIN;

ANIMAL CELL; ARTICLE; CELL ASSAY; CONTROLLED STUDY; COST BENEFIT ANALYSIS; CRYOPRESERVATION; ENZYME LINKED IMMUNOSORBENT ASSAY; GENETIC TRANSFECTION; HUMAN; HUMAN CELL; LARGE SCALE PRODUCTION; NONHUMAN; PRIORITY JOURNAL;

CELL CULTURE TECHNIQUES; CELL LINE, TRANSFORMED; CRYOPRESERVATION; ENZYME-LINKED IMMUNOSORBENT ASSAY; HEK293 CELLS; HUMANS; PROTEIN KINASE INHIBITORS; PROTEIN KINASES; PROTO-ONCOGENE PROTEINS C-MYC; REPRODUCIBILITY OF RESULTS; SENSITIVITY AND SPECIFICITY; TRANSFECTION;

EID: 80053535482     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057111415524     Document Type: Article

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