Change in conformation with reduction of α-helix content causes loss of neutrophil binding activity in fully cytotoxic shiga toxin

Journal of Biological Chemistry

Volumn 286, Issue 40, 2011, Pages 34514-34521

  Brigotti, Maurizio ^a   Carnicelli, Domenica ^a   Arfilli, Valentina ^a   Rocchi, Laura ^a   Ricci, Francesca ^b   Pagliaro, Pasqualepaolo ^b   Tazzari, Pier Luigi ^b   Vara, Antonio González ^a   Amelia, Matteo ^a   Manoli, Francesco ^c   Monti, Sandra ^c  

^a UNIVERSITY OF BOLOGNA   (Italy)

^b UNIVERSITY OF BOLOGNA   (Italy)

^c Istituto per la Sintesi Organica e la Fotoreattività   (Italy)

Author keywords

[No Author keywords available]

Indexed keywords

BINDING ACTIVITIES; CONFORMATIONAL CHANGE; CYTOKINES; CYTOTOXIC; GLOBOTRIAOSYLCERAMIDE; HELIX CONTENT; HUMAN ENDOTHELIAL CELLS; HYDROPHOBIC TRYPTOPHAN RESIDUES; MULTI-STEP PURIFICATION; POLYMORPHONUCLEAR LEUKOCYTES; SHIGA TOXIN; SINGLE-STEP; STRUCTURAL DATA; TARGET CELLS;

AFFINITY CHROMATOGRAPHY; AMINO ACIDS; CELL DEATH; CONFORMATIONS; ENDOTHELIAL CELLS; ESCHERICHIA COLI; METABOLITES;

TOXIC MATERIALS;

VEROTOXIN 1; VEROTOXIN 2;

ALPHA HELIX; ARTICLE; BINDING AFFINITY; CONFORMATIONAL TRANSITION; CONTROLLED STUDY; CYTOTOXICITY; ENZYME ACTIVITY; ESCHERICHIA COLI; HUMAN; HUMAN CELL; NEUTROPHIL; PATHOGENESIS; PRIORITY JOURNAL; PROTEIN CONFORMATION; PROTEIN FUNCTION; PROTEIN SYNTHESIS;

ADENINE; BACTERIAL TOXINS; CIRCULAR DICHROISM; ENDOTHELIAL CELLS; ESCHERICHIA COLI; FLUORESCENT DYES; HEMOLYTIC-UREMIC SYNDROME; HUMANS; KINETICS; NEUTROPHILS; PROTEIN CONFORMATION; PROTEIN STRUCTURE, SECONDARY; RICIN; SHIGA TOXIN; SHIGA TOXIN 1; SHIGA TOXINS; UMBILICAL VEINS;

ESCHERICHIA COLI;

EID: 80053428358     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M111.255414     Document Type: Article

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