Cystic fibrosis and the relationship between mucin and chloride secretion by cultures of human airway gland mucous cells

American Journal of Physiology - Lung Cellular and Molecular Physiology

Volumn 301, Issue 4, 2011, Pages

  Finkbeiner, Walter E ^a   Zlock, Lorna T ^a   Morikawa, Masatoshi ^a   Lao, Anna Y ^a   Dasari, Vijay ^a   Widdicombe, Jonathan H ^b  

^a UNIVERSITY OF CALIFORNIA   (United States)

^b UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

Bronchi; Ion transport; Mucus; Trachea

Indexed keywords

ADENOSINE TRIPHOSPHATE; CHLORIDE; EFENAMIC ACID; ISOPRENALINE; METHACHOLINE; MUCIN; MUCIN 1; MUCIN 4; MUCIN 5B; MUCIN 8; PHENYLEPHRINE;

AIRWAY; ARTICLE; BETA ADRENERGIC STIMULATION; CHEMICAL ANALYSIS; CONTROLLED STUDY; CYSTIC FIBROSIS; DISEASE ASSOCIATION; HUMAN; HUMAN CELL; HUMAN CELL CULTURE; HUMAN TISSUE; IMMUNOHISTOCHEMISTRY; MUCOUS CELL; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN SECRETION; TRACHEA; TRACHEOBRONCHIAL TREE;

ADENOSINE TRIPHOSPHATE; ADRENERGIC ALPHA-1 RECEPTOR AGONISTS; ADRENERGIC BETA-AGONISTS; ADULT; ANTHRANILIC ACIDS; BRONCHI; BRONCHOCONSTRICTOR AGENTS; CALCIUM CHANNEL BLOCKERS; CHLORIDES; CYSTIC FIBROSIS; CYSTIC FIBROSIS TRANSMEMBRANE CONDUCTANCE REGULATOR; EXOCRINE GLANDS; HUMANS; ION TRANSPORT; ISOPROTERENOL; METHACHOLINE CHLORIDE; MUCINS; PHENYLEPHRINE; PRIMARY CELL CULTURE; RESPIRATORY MUCOSA; TRACHEA;

EID: 80053370174     PISSN: 10400605     EISSN: 15221504     Source Type: Journal    
DOI: 10.1152/ajplung.00210.2010     Document Type: Article

References (70)

1. 2+ uptake in cystic fibrosis airway epithelial cells. Mitochondrion 9: 232-241, 2009.
2. Argüeso P, Balaram M, Spurr-Michaud S, Keutmann HT, Dana MR, Gipson IK. Decreased levels of the goblet cell mucin MUC5AC in tears of patients with Sjögren syndrome. Invest Opthalmol Vis Sci 43: 1004-1011, 2002.
3. Ballard ST, Spadafora D. Fluid secretion by submucosal glands of the tracheobronchial airways. Respir Physiol Neurobiol 159: 271-277, 2007.
4. Ballard ST, Trout L, Mehta A, Inglis SK. Liquid secretion inhibitors reduce mucociliary transport in glandular airways. Am J Physiol Lung Cell Mol Physiol 283: L329-L335, 2002.
5. Bernacki SH, Nelson AL, Abdullah L, Sheehan JK, Harris A, Davis CW, Randell SH. Mucin gene expression during differentiation of human airway epithelia in vitro. Am J Respir Cell Mol Biol 20: 595-604, 1999.
6. Bidlingmeyer A, Cohen S, Tarvin T. Rapid analysis of amino acids using pre-column derivatization. J Chromatogr 336: 93-104, 1984.
7. Borthwick DW, Shahbazian M, Drantz QT, Dorin JR, Randell SH. Evidence for stem-cell niches in the tracheal epithelium. Am J Respir Cell Mol Biol 24: 662-670, 2001.
8. + channel subunits in normal and cystic fibrosis airways. Am J Physiol Cell Physiol 269: C511-C518, 1995.
9. Cao Y, Blohm D, Ghadimi BM, Stosiek P, Xing PX, Karsten U. Mucins (MUC1 and MUC3) of gastrointestinal and breast epithelia reveal different and heterogeneous tumor-associated aberrations in glycosylation. J Histochem Cytochem 45: 1547-1557, 1997.
10. Chen Y, Zhao YH, Kalaslavadi TB, Hamati E, Nehrke K, Le AD, Ann DK, Wu R. Genome-wide search and identification of a novel gel-forming mucin MUC19/Muc19 in glandular tissues. Am J Respir Cell Mol Biol 30: 155-165, 2004.
11. Coleman DL, Tuet IK, Widdicombe JH. Electrical properties of dog tracheal epithelial cell grown in monolayer culture. Am J Physiol Cell Physiol 246: C355-C359, 1984.
12. Dasari VK, Goharderakhshan RZ, Perinchery G, Li LC, Tanaka Y, Alonzo J, Dahlaya R. Expression analysis of Y chromosome genes in human prostate cancer. J Urol 165: 1335-1341, 2001.
13. Davies JR, Kirkham S, Svitacheva N, Thornton DJ, Carlstedt I. MUC16 is produced in tracheal surface epithelium and submucosal glands and is present in secretions from normal human airway and cultured bronchial epithelial cells. Int J Biochem Cell Biol 39: 1943-1954, 2007.
14. Englehardt JF, Yankaskas JR, Ernst SA, Yang Y, Marino CR, Boucher RC, Cohn JA, Wilson JM. Submucosal glands are the predominant site of CFTR expression in the human bronchus. Nat Genet 2: 240-247, 1992.
15. Fahy JV, Dickey BF. Airway mucus function and dysfunction. N Engl J Med 363: 2233-2247, 2010.
16. Finkbeiner WE. Pathophysiology of tracheobronchial gland cells. Respir Physiol 118: 77-83, 1999.
17. Finkbeiner WE, Basbaum CB. Monoclonal antibodies directed against human airway secretions: localization and characterization of antigens. Am J Pathol 131: 290-297, 1988.
18. Finkbeiner WE, Carrier SD, Teresi CE. Reverse transcription-polymerase chain reaction (RT-PCR) phenotypic analysis of cell cultures of human tracheal epithelium, tracheobronchial glands and lung carcinomas. Am J Respir Cell Mol Biol 9: 547-556, 1993.
19. Finkbeiner WE, Shen BQ, Widdicombe JH. Chloride secretion and function of serous and mucous cells of human airway glands. Am J Physiol Lung Cell Mol Physiol 267: L206-L210, 1994.
20. Finkbeiner WE, Zlock LT, Mehdi I, Widdicombe JH. Cultures of human tracheobronchial gland cells of mucous or serous phenotype. In Vitro Cell Dev Biol Anim 46: 450-456, 2010.
21. Finne J, Krusius T. Preparation and fractionation of glycopeptides. Methods Enzymol 83: 269-277, 1982.
22. Fischer H, Illek B, Sachs L, Finkbeiner WE, Widdicombe JH. CFTR and calcium-activated channels in primary cultures of human airway gland cells of serous and mucous phenotype. Am J Physiol Lung Cell Mol Physiol 299: L585-L594, 2010.
23. Gipson IK, Spurr-Michaud S, Argüeso P, Tisdale A, Ng TF, Russo CL. Mucin gene expression in immortalized human corneal-limbal and conjunctival epithelial cell lines. Invest Opthalmol Vis Sci 44: 2496-24506, 2003.
24. Gipson IK, Spurr-Michaud S, Moccia R, Zhan Q, Toribara N, Ho SB, Gargiulo AR, Hill JA 3rd. MUC4 and MUC5B transcripts are the prevalent mucin messenger ribonucleic acids of the human endocervix. Biol Reprod 60: 58-64, 1999.
25. - secretion in nasal epithelia of CF mice. Am J Physiol Cell Physiol 266: C1478-C1483, 1994.
26. Hattrup CL, Gendler SJ. Structure and function of the cell surface (tethered) mucins. Annu Rev Physiol 70: 431-457, 2008.
27. Higuchi T, Orita T, Nakanishi S, Katsuya K, Watanabe H, Yamasaki Y, Waga I, Nanayama T, Yamamoto Y, Munger W, Sun HW, Falk RJ, Jennetee JC, Alcorta DA, Li H, Yamamoto T, Saito Y, Nakamura M. Molecular cloning, genomic structure, and expression analysis of MUC20, a novel mucin protein, up-regulated in injured kidney. J Biol Chem 279: 1968-1979, 2004.
28. Hofmann K, Finn FM, Kiso Y. Avidin-biotin affinity columns. General methods for attaching biotin to peptides and proteins. J Am Chem Soc 100: 3585-3590, 1978.
29. Hovenberg HW, Davies JR, Carlstedt I. Different mucins are produced by the surface epithelium and the submucosa in human trachea: identification of MUC5AC as a major mucin from the goblet cells. Biochem J 15: 319-324, 1996.
30. Hovingh P, Linker A. The enzymatic degradation of heparin and heparitin sulfate. 3. Purification of heparitinase and heparinase from flavobacteria. J Biol Chem 245: 6170-6175, 1970.
31. Jacquot J, Puchelle E, Hinnrasky J, Fuchey C, Bettinger C, Spilmont C, Bonnet N, Dieterle A, Dreyer D, Pavirani A, Dalemans W. Localization of the cystic fibrosis transmembrane conductance regulator in airway secretory glands. Eur Respir J 6: 169-176, 1993.
32. - movements in human colonic epithelial cells (HT29-Cl.16E) Biochem J 304: 675-679, 1994.
33. +] and pH but elevated viscosity. Proc Natl Acad Sci USA 98: 8119-8123, 2001.
34. Jiang C, Finkbeiner WE, Widdicombe JH, McCray PBJ, Miller SS. Altered fluid transport across airway epithelium in cystic fibrosis. Science 262: 424-427, 1993.
35. Johnson LG, Boyles SE, Wilson J, Boucher RC. Normalization of raised sodium absorption and raised Ca-mediated chloride secretion by adenovirus- mediated expression of cystic fibrosis transmembrane conductance regulator in primary human cystic fibrosis airway epithelial cells. J Clin Invest 95: 1377-1382, 1995.
36. Joo NS, Wu JV, Krouse ME, Saenz Y, Wine JJ. Optical method for quantifying rates of mucus secretion from single submucosal glands. Am J Physiol Lung Cell Mol Physiol 281: L458-L468, 2001.
37. Kim KC. Possible requirement of collagen gel substratum for production of mucin-like glycoproteins by rabbit tracheal epithelial cells in culture. In Vitro 21: 617-621, 1985.
38. Kreda SM, Mall M, Mengos A, Rochelle L, Yankaskas J, Riordan JR, Boucher RC. Characterization of wild-type and deltaF508 cystic fibrosis transmembrane regulator in human respiratory epithelia. Mol Biol Cell 16: 2154-2167, 2005.
39. Lee RJ, Foskett JK. cAMP-activated Ca2 signaling is required for CFTR-mediated serous cell fluid secretion in porcine and human airways. J Clin Invest 120: 3137-3148, 2010.
40. 2+-stimulated fluid secretion by porcine bronchial submucosal gland serous acinar cells. Am J Physiol Lung Cell Mol Physiol 298: L210-L231, 2010.
41. 2+-evoked fluid secretion by murine nasal submucosal gland serous acinar cells. J Physiol 582: 1099-1124, 2007.
42. Leikauf GD, Ueki IF, Nadel JA. Autonomic regulation of viscoelasticity of cat tracheal gland secretions. J Appl Physiol 56: 426-430, 1984.
43. Lethem MI, Dowell ML, Van Scott M, Yankaskas JR, Egan T, Boucher RC, Davis CW. Nucleotide regulation of goblet cells in human airway epithelial explants: normal exocytosis in cystic fibrosis. Am J Respir Cell Mol Biol 9: 315-322, 1993.
44. Martínez-Antón A, de Bolós C, Garrido M, Foca-Ferrer J, Barranco C, Alobid I, Xaubet A, Picado C, Mullol J. Mucin genes have different expression patterns in healthy and diseased upper airway mucosa. Clin Exp Allergy 36: 448-457, 2006.
45. Meyrick B, Sturgess JM, Reid L. A reconstruction of the duct system and secretory tubules of the human bronchial submucosal gland. Thorax 24: 729-736, 1969.
46. Ohya T, Kaneko Y. Novel hyaluronidase from streptomyces. Biochim Biophys Acta 198: 607-609, 1970.
47. Paul E, Lee DI, Hyun SW, Gendler SJ, Kim KC. Identification and characterization of high molecular-mass mucin-like glycoproteins in the plasma membrane of airway epithelial cells. Am J Respir Cell Mol Biol 19: 681-690, 1998.
48. Plummer THJ, Elder JH, Alexander S, Phelan AW, Tarentino AL. Demonstration of peptide:N-glycosidase F activity in endo-β-N-acetylglucosaminidase F preparations. J Biol Chem 259: 10700-10704, 1984.
49. Puchelle E, Gaillard D, Ploton D, Hinnrasky J, Fuchey C, Boutterin MC, Jacquot J, Dreyer D, Pavirani A, Dalemans W. Differential localization of the cystic fibrosis transmembrane conductance regulator in normal and cystic fibrosis airway epithelium. Am J Respir Cell Mol Biol 7: 485-491, 1992.
50. Rogers DF. Airway goblet cells: responsive and adaptable front-line defenders. Eur Respir J 7: 1690-1706, 1994.
51. Rose MC. Characterization of human tracheobronchial mucin glycoproteins. Methods Enzymol 179: 3-17, 1989.
52. Rose MC, Voynow JA. Respiratory tract mucin genes and mucin glycoproteins in health and disease. Physiol Rev 86: 245-278, 2006.
53. Russo CL, Spurr-Michaud S, Tisdale A, Pudney J, Anderson D, Gipson IK. Mucin gene expression in human male urogenital tract epithelia. Hum Reprod 21: 2783-2793, 2006.
54. Shankar V, Pichan P, Reddy RL, Tonk V, Nowak N, Sait SNJ, Shows TB, Schultz RE, Gotway G, Elkins RC, Gilmore MS, Sachdev GP. Chromosomal localization of a human mucin gene (MUC8) and cloning of the cDNA corresponding to the carboxy terminus. Am J Respir Cell Mol Biol 16: 232-241, 1997.
55. Sharma P, Dudus L, Nielsen PA, Clausen H, Yankaskas JR, Hollingsworth MA, Engelhardt JF. MUC5B and MUC7 are differentially expressed in mucous and serous cells of submucosal glands in human bronchial airways. Am J Respir Cell Mol Biol 19: 30 -37, 1998.
56. Sheehan JK, Kesimer M, Pickles R. Innate immunity and mucus structure and function. Novartis Found Symp 279: 155-166; discussion 167-169, 2006.
57. Shelhamer JH, Marom Z, Kaliner M. Immunologic and neuropharmacologic stimulation of mucous glycoprotein release from human airways in vitro. J Clin Invest 66: 1400-1408, 1980.
58. Shelhamer JH, Marom Z, Logun C, Kaliner M. Human respiratory mucous glycoproteins. Exp Lung Res 7: 149-162, 1984.
59. Shimura S, Sasaki T, Nagaki M, Takishima T, Shirato K. Extracellular ATP regulation of feline tracheal submucosal gland secretion. Am J Physiol Lung Cell Mol Physiol 267: L159-L164, 1994.
60. Sommerhoff CP, Finkbeiner WE. Human tracheobronchial submucosal gland cells in culture. Am J Cell Respir Mol Biol 2: 41-50, 1990.
61. Steiger D, Fahy J, Boushey H, Finkbeiner WE, Basbaum C. Use of mucin antibodies and cDNA probes to quantify hypersecretion in vivo in human airways. Am J Respir Cell Mol Biol 10: 538 -545, 1994.
62. Voynow JA, Gendler SJ, Rose MC. Regulation of mucin genes in chronic inflammatory airway diseases. Am J Respir Cell Mol Biol 34: 661-665, 2006.
63. Welsh MJ, McCann JD. Intracellular calcium regulates basolateral potassium channels in a chloride-secreting epithelium. Proc Natl Acad Sci USA 82: 8823-8826, 1985.
64. Widdicombe JH. Regulation of the depth and composition of airway surface liquid. J Anat 201: 313-318, 2002.
65. Widdicombe JH, Miller SS, Finkbeiner WE. Altered regulation of airway fluid content in cystic fibrosis. In: Cystic Fibrosis-Current Topics, edited by Dodge JA, Brock DJH, Widdicombe JH. Chichester, UK: Wiley, 1994, p. 109-129.
66. Wine JJ, Joo NS. Submucosal glands and airway defense. Proc Am Thorac Soc 1: 47-53, 2004.
67. Wu JV, Krouse ME, Wine JJ. Acinar origin of CFTR-dependent airway submucosal gland fluid secretion. Am J Physiol Lung Cell Mol Physiol 292: L304-L311, 2007.
68. Yamaya M, Finkbeiner WE, Widdicombe JH. Altered ion transport by tracheal glands in cystic fibrosis. Am J Physiol Lung Cell Mol Physiol 261: L491-L494, 1991.
69. Yamaya M, Finkbeiner WE, Widdicombe JH. Ion transport by cultures of human tracheobronchial submucosal glands. Am J Physiol Lung Cell Mol Physiol 261: L485-L490, 1991.
70. Yamaya M, Ohrui T, Finkbeiner WE, Widdicombe JH. Calciumdependent Cl secretion across cultures of tracheal surface epithelium and glands. Am J Physiol Lung Cell Mol Physiol 265: L170-L177, 1993.