Fusion performance of low-dose recombinant human bone morphogenetic protein 2 and bone marrow-derived multipotent stromal cells in biodegradable scaffolds: A comparative study in a large animal model of anterior lumbar interbody fusion

Spine

Volumn 36, Issue 21, 2011, Pages 1752-1759

  Abbah, Sunny A ^a   Lam, Christopher X F ^b   Ramruttun, Amit K ^a   Goh, James C H ^a,b   Wong, Hee Kit ^a  

^a NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

^b NATIONAL UNIVERSITY OF SINGAPORE   (Singapore)

Author keywords

bioresorbable cages; BMP 2; interbody fusion; lumbar spine; pig; polycaprolactone tricalcium phosphate; stem cells

Indexed keywords

CALCIUM PHOSPHATE; POLYCAPROLACTONE; RECOMBINANT BONE MORPHOGENETIC PROTEIN 2; TISSUE SCAFFOLD;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ANTERIOR SPINE FUSION; ARTICLE; BIODEGRADABILITY; BIOMECHANICS; BONE GRAFT; BONE GROWTH; BONE PROSTHESIS; CONTROLLED STUDY; HEMATOPOIETIC STEM CELL; HISTOPATHOLOGY; INTERMETHOD COMPARISON; MALE; MICRO-COMPUTED TOMOGRAPHY; MULTIPOTENT STEM CELL; NONHUMAN; PRIORITY JOURNAL; SPINE RADIOGRAPHY; SWINE;

ABSORBABLE IMPLANTS; ANIMALS; BIOMECHANICS; BONE MORPHOGENETIC PROTEIN 2; CALCIUM PHOSPHATES; CELLS, CULTURED; HUMANS; ILIUM; LUMBAR VERTEBRAE; MALE; MESENCHYMAL STROMAL CELLS; MODELS, ANIMAL; MULTIPOTENT STEM CELLS; OSSEOINTEGRATION; POLYESTERS; RANGE OF MOTION, ARTICULAR; RECOMBINANT PROTEINS; SPINAL FUSION; SWINE; TIME FACTORS; TISSUE ENGINEERING; TISSUE SCAFFOLDS; TRANSPLANTATION, AUTOLOGOUS; X-RAY MICROTOMOGRAPHY;

EID: 80053363820     PISSN: 03622436     EISSN: 15281159     Source Type: Journal    
DOI: 10.1097/BRS.0b013e31822576a4     Document Type: Article

References (40)

1. Wang JC, Mummaneni PV, Haid RW. Current treatment strategies for the painful lumbar motion segment: posterolateral fusion versus interbody fusion. Spine 2005;30:S33-43. (Pubitemid 41208816)
2. Zdeblick TA, Phillips FM. Interbody cage devices. Spine 2003;28:S2-7. (Pubitemid 36959576)
3. Togawa D, Bauer TW, Lieberman IH, et al. Lumbar intervertebral body fusion cages: histological evaluation of clinically failed cages retrieved from humans. J Bone Joint Surg Am 2004;86-A:70-9. (Pubitemid 38057339)
4. Tullberg T. Failure of a carbon fiber implant. A case report. Spine 1998;23:1804-6. (Pubitemid 28443087)
5. Blumenthal SL, Ohnmeiss DD. Intervertebral cages for degenerative spinal diseases. Spine J 2003;3:301-9. (Pubitemid 38351740)
6. Chau AM, Mobbs RJ. Bone graft substitutes in anterior cervical discectomy and fusion. Eur Spine J 2009;18:449-64.
7. Roberts SJ, Howard D, Buttery LD, Shakesheff KM. Clinical applications of musculoskeletal tissue engineering. Br Med Bull 2008;86:7-22. (Pubitemid 351809633)
8. Vaccaro AR, Singh K, Haid R, et al. The use of bioabsorbable implants in the spine. Spine J 2003;3:227-37. (Pubitemid 38351753)
9. Wuisman PI, Smit TH. Bioresorbable polymers: heading for a new generation of spinal cages. Eur Spine J 2006;15:133-48.
10. Schumann D, Ekaputra AK, Lam CX, et al. Biomaterials/scaffolds. Design of bioactive, multiphasic PCL/collagen type I and type II-PCL-TCP/collagen composite scaffolds for functional tissue engineering of osteochondral repair tissue by using electrospinning and FDM techniques. Methods Mol Med 2007;140 : 101-24.
11. Zein I, Hutmacher DW, Tan KC, et al. Fused deposition modeling of novel scaffold architectures for tissue engineering applications. Biomaterials 2002;23:1169-85. (Pubitemid 33109049)
12. Hutmacher DW. Scaffolds in tissue engineering bone and cartilage. Biomaterials 2000;21:2529-43.
13. Hutmacher DW, Schantz T, Zein I, et al. Mechanical properties and cell cultural response of polycaprolactone scaffolds designed and fabricated via fused deposition modeling. J Biomed Mater Res 2001;55:203-16. (Pubitemid 32198503)
14. Abbah SA, Lam CX, Hutmacher DW, et al. Biological performance of a polycaprolactone-based scaffold used as fusion cage device in a large animal model of spinal reconstructive surgery. Biomaterials 2009;30:5086-93.
15. Sawyer AA, Song SJ, Susanto E, et al. The stimulation of healing within a rat calvarial defect by mPCL-TCP/collagen scaffolds loaded with rhBMP-2. Biomaterials 2009;30:2479-88.
16. See EY, Toh SL, Goh JC. Multilineage potential of bone-marrowderived mesenchymal stem cell cell sheets: implications for tissue engineering. Tissue Eng Part A 2010;16:1421-31.
17. Toth JM, Wang M, Scifert JL, et al. Evaluation of 70/30 D,LPLa for use as a resorbable interbody fusion cage. Orthopedics 2002;25:s1131-40. (Pubitemid 35276914)
18. Panjabi MM. Biomechanical evaluation of spinal fixation devices: I. A conceptual framework. Spine 1988;13:1129-34.
19. Lysack JT, Yen D, Dumas GA. In vitro fl exibility of an experimental pedicle screw and plate instrumentation system on the porcine lumbar spine. Med Eng Phys 2000;22:461-8. (Pubitemid 32112670)
20. Neen D, Noyes D, Shaw M, et al. Healos and bone marrow aspirate used for lumbar spine fusion: a case controlled study comparing healos with autograft. Spine 2006;31:E636-40.
21. Tsantrizos A, Baramki HG, Zeidman S, et al. Segmental stability and compressive strength of posterior lumbar interbody fusion implants . Spine 2000;25:1899-907. (Pubitemid 30626068)
22. Hojo Y, Kotani Y, Ito M, et al. A biomechanical and histological evaluation of a bioresorbable lumbar interbody fusion cage. Biomaterials 2005;26:2643-51. (Pubitemid 39600716)
23. van Dijk M, Smit TH, Burger EH, et al. Bioabsorbable poly-L-lactic acid cages for lumbar interbody fusion: three-year follow-up radiographic, histologic, and histomorphometric analysis in goats. Spine 2002;27:2706-14. (Pubitemid 35425325)
24. Smith AJ, Arginteanu M, Moore F, et al. Increased incidence of cage migration and nonunion in instrumented transforaminal lumbar interbody fusion with bioabsorbable cages. J Neurosurg Spine 2010;13:388-93.
25. Pflugmacher R, Schleicher P, Gumnior S, et al. Biomechanical comparison of bioabsorbable cervical spine interbody fusion cages. Spine 2004;29:1717-22. (Pubitemid 39079912)
26. Slivka MA, Spenciner DB, Seim HB III, et al. High rate of fusion in sheep cervical spines following anterior interbody surgery with absorbable and nonabsorbable implant devices. Spine 2006;31 : 2772-7. (Pubitemid 44772775)
27. Smit TH, Engels TA, Wuisman PI, et al. Time-dependent mechanical strength of 70/30 Poly(L, DL-lactide): shedding light on the premature failure of degradable spinal cages. Spine 2008;33 : 14-18.
28. Engels TA, Sontjens SH, Smit TH, et al. Time-dependent failure of amorphous polylactides in static loading conditions. J Mater Sci Mater Med 2010;21:89-97.
29. Zhang H, Sucato DJ, Welch RD. Recombinant human bone morphogenic protein-2-enhanced anterior spine fusion without bone encroachment into the spinal canal: a histomorphometric study in a thoracoscopically instrumented porcine model. Spine 2005;30 : 512-18. (Pubitemid 40293837)
30. Sucato DJ, Hedequist D, Zhang H, et al. Recombinant human bone morphogenetic protein-2 enhances anterior spinal fusion in a thoracoscopically instrumented animal model. J Bone Joint Surg Am 2004;86-A:752-62. (Pubitemid 38436718)
31. Smucker JD, Rhee JM, Singh K, et al. Increased swelling complications associated with off-label usage of rhBMP-2 in the anterior cervical spine. Spine 2006;31:2813-19. (Pubitemid 44772783)
32. Lam CX, Savalani MM, Teoh SH, et al. Dynamics of in vitro polymer degradation of polycaprolactone-based scaffolds: accelerated versus simulated physiological conditions. Biomed Mater 2008;3:034108.
33. Yeo A, Rai B, Sju E, et al. The degradation profile of novel, bioresorbable PCL-TCP scaffolds: an in vitro and in vivo study. J Biomed Mater Res A 2008;84:208-18. (Pubitemid 350244262)
34. Yeo A, Sju E, Rai B, et al. Customizing the degradation and loadbearing profile of 3D polycaprolactone-tricalcium phosphate scaffolds under enzymatic and hydrolytic conditions. J Biomed Mater Res B Appl Biomater 2008;87:562-9.
35. Zhou Y, Hutmacher DW, Varawan SL, et al. In vitro bone engineering based on polycaprolactone and polycaprolactone-tricalcium phosphate composites. Polymer International 2007;56 : 333-42.
36. Lam CX, Teoh SH, Hutmacher DW. Comparison of the degradation of polycaprolactone and polycaprolactone-(α-tricalcium phosphate) scaffolds in alkaline medium. Polymer International 2007;56:718-28. (Pubitemid 46795738)
37. Qian Y, Lin Z, Chen J, et al. Natural bone collagen scaffold combined with autologous enriched bone marrow cells for induction of osteogenesis in an ovine spinal fusion model. Tissue Eng Part A 2009;15:3547-58.
38. Hagi TT, Wu G, Liu Y, et al. Cell-mediated BMP-2 liberation promotes bone formation in a mechanically unstable implant environment . Bone 2010;46:1322-7.
39. Carter DR, Wong M. Mechanical stresses and endochondral ossification in the chondroepiphysis. J Orthop Res 1988;6:148-54. (Pubitemid 18010244)
40. Lam CX, Hutmacher DW, Schantz JT, et al. Evaluation of polycaprolactone scaffold degradation for 6 months in vitro and in vivo. J Biomed Mater Res A 2009;90:906-19.