Rem2-targeted shRNAs reduce frequency of miniature excitatory postsynaptic currents without altering voltage-gated Ca 2+ currents

PLoS ONE

Volumn 6, Issue 9, 2011, Pages

  Wang, Hong Gang ^a   Wang, Chuan ^a,b   Pitt, Geoffrey S ^a  

^a DUKE UNIVERSITY MEDICAL CENTER   (United States)

^b HEBEI MEDICAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

GUANOSINE TRIPHOSPHATASE; PROTEIN REM2; SHORT HAIRPIN RNA; UNCLASSIFIED DRUG; VOLTAGE GATED CALCIUM CHANNEL; CALCIUM; CALCIUM CHANNEL; MONOMERIC GUANINE NUCLEOTIDE BINDING PROTEIN; REM2 PROTEIN, RAT; SMALL INTERFERING RNA;

ANIMAL CELL; ARTICLE; CALCIUM CURRENT; CALCIUM HOMEOSTASIS; CONTROLLED STUDY; ENZYME ACTIVITY; EXCITATORY POSTSYNAPTIC POTENTIAL; FREQUENCY MODULATION; GENE EXPRESSION; GENE SILENCING; GENETIC TRANSFECTION; HIPPOCAMPUS; HUMAN; HUMAN CELL; IN VITRO STUDY; MINIATURE EXCITATORY POSTSYNAPTIC CURRENT; MOLECULAR MECHANICS; NERVE CELL CULTURE; NEWBORN; NONHUMAN; PROTEIN EXPRESSION; PROTEIN TARGETING; RAT; SYNAPTOGENESIS; ANIMAL; CELL STRAIN HEK293; CYTOLOGY; GENETICS; HOMEOSTASIS; METABOLISM; NERVE CELL; SPRAGUE DAWLEY RAT; SYNAPSE;

RATTUS;

ANIMALS; CALCIUM; CALCIUM CHANNELS; EXCITATORY POSTSYNAPTIC POTENTIALS; GENE KNOCKDOWN TECHNIQUES; HEK293 CELLS; HIPPOCAMPUS; HOMEOSTASIS; HUMANS; MONOMERIC GTP-BINDING PROTEINS; NEURONS; RATS; RATS, SPRAGUE-DAWLEY; RNA, SMALL INTERFERING; SYNAPSES;

EID: 80053333898     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0025741     Document Type: Article

References (26)

1. van Daalen E, Kemner C, Dietz C, Swinkels SH, Buitelaar JK, et al. (2009) Inter-rater reliability and stability of diagnoses of autism spectrum disorder in children identified through screening at a very young age. Eur Child Adolesc Psychiatry 18: 663-674.
2. Peterson BZ, DeMaria CD, Adelman JP, Yue DT, (1999) Calmodulin is the Ca2+ Sensor for Ca2+-Dependent Inactivation of L-type Calcium Channels [published erratum appears in Neuron 1999 Apr;22(4):following 893]. Neuron 22: 549-558.
3. Zühlke RD, Pitt GS, Deisseroth K, Tsien RW, Reuter H, (1999) Calmodulin supports both inactivation and facilitation of L-type calcium channels. Nature 399: 159-162.
4. Lee A, Wong ST, Gallagher D, Li B, Storm DR, et al. (1999) Ca2+/calmodulin binds to and modulates P/Q-type calcium channels. Nature 399: 155-159.
5. Flynn R, Zamponi GW, (2010) Regulation of calcium channels by RGK proteins. Channels 4: 434-439.
6. Beguin P, Nagashima K, Gonoi T, Shibasaki T, Takahashi K, et al. (2001) Regulation of Ca2+ channel expression at the cell surface by the small G-protein kir/Gem. Nature 411: 701-706.
7. Finlin BS, Crump SM, Satin J, Andres DA, (2003) Regulation of voltage-gated calcium channel activity by the Rem and Rad GTPases. Proc Natl Acad Sci U S A 100: 14469-14474.
8. Seu L, Pitt GS, (2006) Dose-dependent and Isoform-specific Modulation of Ca2+ Channels by RGK GTPases. J Gen Physiol 128: 605-613.
9. Fan M, Buraei Z, Luo HR, Levenson-Palmer R, Yang J, (2010) Direct inhibition of P/Q-type voltage-gated Ca2+ channels by Gem does not require a direct Gem/Cavbeta interaction. Proc Natl Acad Sci U S A 107: 14887-14892.
10. Ward Y, Yap SF, Ravichandran V, Matsumura F, Ito M, et al. (2002) The GTP binding proteins Gem and Rad are negative regulators of the Rho-Rho kinase pathway. J Cell Biol 157: 291-302.
11. Maguire J, Santoro T, Jensen P, Siebenlist U, Yewdell J, et al. (1994) Gem: an induced, immediate early protein belonging to the Ras family. Science 265: 241-244.
12. Reynet C, Kahn CR, (1993) Rad: a member of the Ras family overexpressed in muscle of type II diabetic humans. Science 262: 1441-1444.
13. Opatowsky Y, Sasson Y, Shaked I, Ward Y, Chomsky-Hecht O, et al. (2006) Structure-function studies of the G-domain from human gem, a novel small G-protein. FEBS Lett 580: 5959-5964.
14. Chang L, Zhang J, Tseng Y-H, Xie C-Q, Ilany J, et al. (2007) Rad GTPase Deficiency Leads to Cardiac Hypertrophy. Circulation 116: 2976-2983.
15. Wang G, Zhu X, Xie W, Han P, Li K, et al. (2010) Rad As a Novel Regulator of Excitation-Contraction Coupling and {beta}-Adrenergic Signaling in Heart. Circ Res 106: 317-327.
16. Finlin BS, Shao H, Kadono-Okuda K, Guo N, Andres DA, (2000) Rem2, a new member of the Rem/Rad/Gem/Kir family of Ras-related GTPases. Biochem J 347 Pt 1: 223-231.
17. Paradis S, Harrar DB, Lin Y, Koon AC, Hauser JL, et al. (2007) An RNAi-based approach identifies molecules required for glutamatergic and GABAergic synapse development. Neuron 53: 217-232.
18. Chen H, Puhl HL, 3rd, Niu SL, Mitchell DC, Ikeda SR, (2005) Expression of Rem2, an RGK family small GTPase, reduces N-type calcium current without affecting channel surface density. J Neurosci 25: 9762-9772.
19. Wang HG, George MS, Kim J, Wang C, Pitt GS, (2007) Ca2+/calmodulin regulates trafficking of Ca(V)1.2 Ca2+ channels in cultured hippocampal neurons. J Neurosci 27: 9086-9093.
20. Yada H, Murata M, Shimoda K, Yuasa S, Kawaguchi H, et al. (2007) Dominant Negative Suppression of Rad Leads to QT Prolongation and Causes Ventricular Arrhythmias via Modulation of L-type Ca2+ Channels in the Heart. Circ Res 101: 69-77.
21. Bekkers JM, Stevens CF, (1990) Presynaptic mechanism for long-term potentiation in the hippocampus. Nature 346: 724-729.
22. Alvarez VA, Ridenour DA, Sabatini BL, (2006) Retraction of Synapses and Dendritic Spines Induced by Off-Target Effects of RNA Interference. J Neurosci 26: 7820-7825.
23. Ghiretti AE, Paradis S, (2011) The GTPase Rem2 regulates synapse development and dendritic morphology. Developmental Neurobiology 71: 374-389.
24. Edel MJ, Menchon C, Menendez S, Consiglio A, Raya A, et al. (2010) Rem2 GTPase maintains survival of human embryonic stem cells as well as enhancing reprogramming by regulating p53 and cyclin D1. GENES & DEVELOPMENT 24: 561-573.
25. Basarsky TA, Parpura V, Haydon PG, (1994) Hippocampal synaptogenesis in cell culture: developmental time course of synapse formation, calcium influx, and synaptic protein distribution pp. 6402-6411.
26. Greer PL, Greenberg ME, (2008) From Synapse to Nucleus: Calcium-Dependent Gene Transcription in the Control of Synapse Development and Function. Neuron 59: 846-860.