Assays for Structure-Selective DNA Endonucleases

Methods in Molecular Biology

Volumn 745, Issue , 2011, Pages 345-362

  Wright, William D ^a   Ehmsen, Kirk T ^a   Heyer, Wolf Dietrich ^a  

^a UNIVERSITY OF CALIFORNIA   (United States)

Author keywords

DNA joint molecule; endonuclease; flap; Holliday junction; incision site; kinetic analysis; Michaelis Menten analysis; Mus81 Mms4 Eme1; recombination; XPF paralogs

Indexed keywords

CRUCIFORM DNA; DNA; ENDONUCLEASE;

ARTICLE; CHEMISTRY; ENZYME SPECIFICITY; GENETIC RECOMBINATION; GENETICS; METABOLISM;

DNA; DNA, CRUCIFORM; ENDONUCLEASES; RECOMBINATION, GENETIC; SUBSTRATE SPECIFICITY;

EID: 80053331796     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-129-1_20     Document Type: Chapter

References (10)

1. Ciccia, A., McDonald, N., and West, S.C. (2008) Structural and functional relationships of the XPF/MUS81 family of proteins. Annu Rev Biochem 77, 259–287.
2. Heyer, W.D., Ehmsen, K.T., and Solinger, J.A. (2003) Holliday junctions in the eukaryotic nucleus: resolution in sight? Trends Biochem Sci 28, 548–557.
3. Hollingsworth, N.M., and Brill, S.J. (2004) The Mus81 solution to resolution: generating meiotic crossovers without Holliday junctions. Genes Dev 18, 117–125.
4. Mimitou, E.P., and Symington, L.S. (2009) Nucleases and helicases take center stage in homologous recombination. Trends Biochem Sci 34, 264–272.
5. Svendsen, J.M., and Harper, J.W. (2010) GEN1/Yen1 and the SLX4 complex: solutions to the problem of Holliday junction resolution. Genes 24, 521–536.
6. Ehmsen, K.T., and Heyer, W.D. (2008) Saccharomyces cerevisiae Mus81-Mms4 is a catalytic, DNA structure-selective endonuclease. Nucleic Acids Res 36, 2182–2195.
7. Ehmsen, K.T., and Heyer, W.D. (2009) A junction branch point adjacent to a DNA backbone nick directs substrate cleavage by Saccharomyces cerevisiae Mus81-Mms4. Nucleic Acids Res 37, 2026–2036.
8. Parkin, K. (2002) Enzyme kinetics: a modern approach (Malden, MA: Wiley-Interscience).
9. Cornish-Bowden, A. (1995) Fundamentals of enzyme kinetics (London: Portland Press Limited).
10. Segel, I.H. (1993) Enzyme kinetics: behavior and analysis of rapid equilibrium and steady state enzyme systems (New York, NY: Wiley-Interscience).