In Vivo Site-Specific Mutagenesis and Gene Collage Using the Delitto Perfetto System in Yeast Saccharomyces cerevisiae

Methods in Molecular Biology

Volumn 745, Issue , 2011, Pages 173-191

  Stuckey, Samantha ^a   Mukherjee, Kuntal ^a   Storici, Francesca ^a  

^a GEORGIA INSTITUTE OF TECHNOLOGY   (United States)

Author keywords

delitto perfetto system double strand break; DNA modification; DNA oligonucleotides; gene collage; gene targeting; site directed mutagenesis; yeast Saccharomyces cerevisiae

Indexed keywords

ARTICLE; DOUBLE STRANDED DNA BREAK; GENETICS; GENOTYPE; METHODOLOGY; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE; SITE DIRECTED MUTAGENESIS;

DNA BREAKS, DOUBLE-STRANDED; GENOTYPE; MUTAGENESIS, SITE-DIRECTED; POLYMERASE CHAIN REACTION; SACCHAROMYCES CEREVISIAE;

EID: 80053323743     PISSN: 10643745     EISSN: 19406029     Source Type: Book Series    
DOI: 10.1007/978-1-61779-129-1_11     Document Type: Chapter

References (11)

1. Sherman, F. (2002) Getting started with yeast. Methods Enzymol 350, 3–41.
2. Dujon, B. (1996) The yeast genome project: what did we learn? Trends Genet 12, 263– 270.
3. Oliver, S.G. (1996) From DNA sequence to biological function. Nature 379, 653–654.
4. Winzeler, E.A., and Davis, R.W. (1997) Functional analysis of the yeast genome. Curr Opin Genet Dev 7, 771–776.
5. Resnick, M.A., and Cox, B.S. (2000) Yeast as an honorary mammal. Mutat Res 451, 1–11.
6. Wach, A., Brachat, A., Pohlmann, R., and Philippsen, P. (1994) New heterologous modules for classical or PCR-based gene disruptions in Saccharomyces cerevisiae. Yeast 10, 1793–1808.
7. Storici, F., Lewis, L.K., and Resnick, M.A. (2001) In vivo site-directed mutagenesis using oligonucleotides. Nat Biotechnol 19, 773–776.
8. Storici, F., and Resnick, M.A. (2003) Delitto perfetto targeted mutagenesis in yeast with oligonucleotides. In Genetic engineering, principle and methods, Vol. 25 J.K. Setlow, ed. (Upton, NY: Kluwer Academic/Plenum Publisher), pp. 189–207.
9. Storici, F., Durham, C., Gordenin, D., and Resnick, M. (2003) Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast. Proc Natl Acad Sci 100, 14994– 14999.
10. Storici, F., Snipe, J., Chan, G., Gordenin, G., and Resnick, M. (2006) Conservative repair of a chromosomal double-strand break by single-strand DNA through two steps of annealing. Mol Cell Biol 26, 7645–7657.
11. Storici, F., and Resnick, M. (2006) The delitto perfetto approach to in vivo site-directed mutagenesis and chromosome rearrangements with synthetic oligonucleotides in yeast. Methods Enzymol 409, 329–345.