New fluorescent substrates of microbial transglutaminase and its application to peptide tag-directed covalent protein labeling

Methods in Molecular Biology

Volumn 751, Issue , 2011, Pages 81-94

  Kamiya, Noriho ^a   Abe, Hiroki ^a  

^a KYUSHU UNIVERSITY   (Japan)

Author keywords

Fluorescent substrate peptide; Peptide tag; Site specific protein modification; Transglutaminase

Indexed keywords

ALKALINE PHOSPHATASE; BUFFER; FLUORESCEIN ISOTHIOCYANATE; PROTEIN GLUTAMINE GAMMA GLUTAMYLTRANSFERASE; 9 FLUORENYLMETHOXYCARBONYL; 9-FLUORENYLMETHOXYCARBONYL; FLUORENE DERIVATIVE; FLUORESCENT DYE; GLUTAMINE; HYBRID PROTEIN; PEPTIDE;

AMINO TERMINAL SEQUENCE; CARBOXY TERMINAL SEQUENCE; CHEMICAL LABELING; CROSS LINKING; DNA TEMPLATE; ELECTROPHORESIS; EXPRESSION VECTOR; FLUORESCENCE IMAGING; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MOLECULAR CLONING; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; PEPTIDE SYNTHESIS; PLASMID; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PROTEIN DETERMINATION; PROTEIN LABELING; REVERSED PHASE HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; SEQUENCE ANALYSIS; STREPTOMYCES; STREPTOMYCES MOBARAENSIS; AMINO ACID SEQUENCE; ARTICLE; BIOCATALYSIS; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; ESCHERICHIA COLI; GENE VECTOR; GENETICS; METABOLISM; METHODOLOGY; STAINING;

ALKALINE PHOSPHATASE; AMINO ACID SEQUENCE; BIOCATALYSIS; ELECTROPHORESIS, POLYACRYLAMIDE GEL; ESCHERICHIA COLI; FLUORENES; FLUORESCEIN-5-ISOTHIOCYANATE; FLUORESCENT DYES; GENETIC VECTORS; GLUTAMINE; PEPTIDES; PLASMIDS; RECOMBINANT FUSION PROTEINS; STAINING AND LABELING; STREPTOMYCES; SUBSTRATE SPECIFICITY; TRANSGLUTAMINASES;

EID: 80053310456     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-151-2_7     Document Type: Chapter

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