Inducible cassette exchange: A rapid and efficient system enabling conditional gene expression in embryonic stem and primary cells

Stem Cells

Volumn 29, Issue 10, 2011, Pages 1580-1588

  Iacovino, Michelina ^a   Bosnakovski, Darko ^b   Fey, Holger ^a   Rux, Danielle ^a   Bajwa, Gagan ^c   Mahen, Elisabeth ^a   Mitanoska, Ana ^a   Xu, Zhaohui ^c   Kyba, Michael ^a,c  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b GOCE DELCEV UNIVERSITY   (Macedonia)

^c UNIVERSITY OF TEXAS SOUTHWESTERN MEDICAL CENTER   (United States)

Author keywords

Cassette exchange recombination; Conditional gene expression; ESCs

Indexed keywords

LENTIVIRUS VECTOR; MYOGENIC FACTOR;

ALLELE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL DIFFERENTIATION; CELL FATE; CONTROLLED STUDY; EMBRYONIC STEM CELL; GENE; GENE EXPRESSION; GENE FUNCTION; GENE LOCUS; GENETIC ANALYSIS; INDUCIBLE CASSETTE EXCHANGE GENE; MALE; MOUSE; MYF5 GENE; NONHUMAN; SKELETAL MUSCLE;

MURINAE; MUS;

EID: 80053233920     PISSN: 10665099     EISSN: None     Source Type: Journal    
DOI: 10.1002/stem.715     Document Type: Article

References (30)

1. Thomson JA, Itskovitz-Eldor J, Shapiro SS et al. Embryonic stem cell lines derived from human blastocysts. Science 1998;282:1145-1147. (Pubitemid 28516247)
2. Evans MJ, Kaufman M. Establishment in culture of pluripotential cells from mouse embryos. Nature 1981;292:154-156. (Pubitemid 11050741)
3. Martin GR. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells. Proc Natl Acad Sci USA 1981;78:7634-7638. (Pubitemid 12186197)
4. Thomas KR, Capecchi MR. Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Cell 1987;51:503-512.
5. Doetschman T, Gregg RG, Maeda N et al. Targeted correction of a mutant HPRT gene in mouse embryonic stem cells. Nature 1987;330:576-578. (Pubitemid 17164356)
6. Zwaka TP, Thomson JA. Homologous recombination in human embryonic stem cells. Nat Biotechnol 2003;21:319-321. (Pubitemid 36314818)
7. Porteus MH, Baltimore D. Chimeric nucleases stimulate gene targeting in human cells. Science 2003;300:763. (Pubitemid 36532103)
8. Radecke S, Radecke F, Cathomen T et al. Zinc-finger nuclease-induced gene repair with oligodeoxynucleotides: Wanted and unwanted target locus modifications. Mol Ther 2010;18:743-753.
9. Bronson SK, Plaehn EG, Kluckman KD et al. Single-copy transgenic mice with chosen-site integration. Proc Natl Acad Sci USA 1996;93:9067-9072. (Pubitemid 26282019)
10. Iacovino M, Hernandez C, Xu Z et al. A conserved role for Hox paralog group 4 in regulation of hematopoietic progenitors. Stem Cells Dev 2009;18:783-792.
11. Kyba M, Perlingeiro RCR, Daley GQ. HoxB4 confers definitive lymphoid-myeloid engraftment potential on embryonic stem cell and yolk sac hematopoietic progenitors. Cell 2002;109:29-37. (Pubitemid 34327514)
12. Agha-Mohammadi S, O'Malley M, Etemad A et al. Second-generation tetracycline-regulatable promoter: Repositioned tet operator elements optimize transactivator synergy while shorter minimal promoter offers tight basal leakiness. J Gene Med 2004;6:817-828. (Pubitemid 40228098)
13. Bosnakovski D, Xu Z, Gang EJ et al. An isogenetic myoblast expression screen identifies DUX4-mediated FSHD-associated molecular pathologies. EMBO J 2008;27:2766-2779.
14. Lois C, Hong EJ, Pease S et al. Germline transmission and tissue-specific expression of transgenes delivered by lentiviral vectors. Science 2002;295:868-872. (Pubitemid 34118370)
15. Urlinger S, Baron U, Thellmann M et al. Exploring the sequence space for tetracycline-dependent transcriptional activators: Novel mutations yield expanded range and sensitivity. Proc Natl Acad Sci USA 2000;97:7963-7968. (Pubitemid 30460756)
16. Heaney JD, Rettew AN, Bronson SK. Tissue-specific expression of a BAC transgene targeted to the Hprt locus in mouse embryonic stem cells. Genomics 2004;83:1072-1082. (Pubitemid 38726055)
17. Touw K, Hoggatt AM, Simon G et al. Hprt-targeted transgenes provide new insights into smooth muscle-restricted promoter activity. Am J Physiol Cell Physiol 2007;292:C1024-C1032. (Pubitemid 46440400)
18. Portales-Casamar E, Swanson DJ, Liu L et al. A regulatory toolbox of MiniPromoters to drive selective expression in the brain. Proc Natl Acad Sci USA 2010;107:16589-16594.
19. Hooper M, Hardy K, Handyside A et al. HPRT-deficient (Lesch-Nyhan) mouse embryos derived from germline colonization by cultured cells. Nature 1987;326:292-295. (Pubitemid 17053752)
20. Wutz A, Rasmussen TP, Jaenisch R. Chromosomal silencing and localization are mediated by different domains of Xist RNA. Nat Genet 2002;30:167-174.
21. Fukushige S, Sauer B. Genomic targeting with a positive-selection lox integration vector allows highly reproducible gene expression in mammalian cells. Proc Natl Acad Sci USA 1992;89:7905-7909.
22. Darabi R, Gehlbach K, Bachoo RM et al. Functional skeletal muscle regeneration from differentiating embryonic stem cells. Nat Med 2008;14:134-143. (Pubitemid 351214552)
23. Rudnicki MA, Schnegelsberg PN, Stead RH et al. MyoD or Myf-5 is required for the formation of skeletal muscle. Cell 1993;75:1351-1359. (Pubitemid 24021909)
24. Ott MO, Bober E, Lyons G et al. Early expression of the myogenic regulatory gene, myf-5, in precursor cells of skeletal muscle in the mouse embryo. Development 1991;111:1097-1107.
25. Tajbakhsh S, Rocancourt D, Cossu G et al. Redefining the genetic hierarchies controlling skeletal myogenesis: Pax-3 and Myf-5 act upstream of MyoD. Cell 1997;89:127-138.
26. Bajard L, Relaix F, Lagha M et al. A novel genetic hierarchy functions during hypaxial myogenesis: Pax3 directly activates Myf5 in muscle progenitor cells in the limb. Genes Dev 2006;20:2450-2464. (Pubitemid 44320392)
27. Mizuno Y, Chang H, Umeda K et al. Generation of skeletal muscle stem/progenitor cells from murine induced pluripotent stem cells. FASEB J 2010;24:2245-2253.
28. Barberi T, Bradbury M, Dincer Z et al. Derivation of engraftable skeletal myoblasts from human embryonic stem cells. Nat Med 2007;13:642-648. (Pubitemid 46828477)
29. Darabi R, Pan W, Bosnakovski D et al. Functional myogenic engraftment from mouse iPS cells. Stem Cell Rev 2011 [Epub ahead of print].
30. Darabi R, Santos FN, Filareto A et al. Assessment of the myogenic stem cell compartment following transplantation of Pax3/Pax7-induced embryonic stem cell-derived progenitors. Stem Cells 2011;29:777-790.