Validation of reference genes in human testis and ejaculate

Andrologia

Volumn 43, Issue 5, 2011, Pages 361-367

  Cavalcanti, M C O ^a   Failling, K ^a   Schuppe, H C ^a   Bergmann, M ^a   Stalf, T ^b   Weidner, W ^a   Steger, K ^a  

^a JUSTUS LIEBIG UNIVERSITY   (Germany)

^b Fertility Center Middle Hessia   (Germany)

Author keywords

Ejaculate; Real time quantitative PCR; Reference gene; Testis biopsy

Indexed keywords

ADENOSINE TRIPHOSPHATE; ADENOSINE TRIPHOSPHATE 5 BETA; BETA ACTIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; HEAT SHOCK PROTEIN; HEAT SHOCK PROTEIN 1BETA; PARAFFIN; UNCLASSIFIED DRUG;

ARTICLE; CLINICAL ARTICLE; CONTROLLED STUDY; CRYOPRESERVATION; GENE EXPRESSION; GENETIC STABILITY; HUMAN; HUMAN TISSUE; MALE; MALE FERTILITY; MALE INFERTILITY; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SPERM; STATISTICAL ANALYSIS; SUBFERTILITY; TESTIS;

BASE SEQUENCE; DNA PRIMERS; DNA, COMPLEMENTARY; EJACULATION; GENE EXPRESSION PROFILING; HUMANS; MALE; REAL-TIME POLYMERASE CHAIN REACTION; SEMEN; TESTIS;

EID: 80053177335     PISSN: 03034569     EISSN: 14390272     Source Type: Journal    
DOI: 10.1111/j.1439-0272.2010.01076.x     Document Type: Article

References (23)

1. Barber RD, Harmer DW, Coleman RA, Clark BJ (2005) GAPDH as a housekeeping gene: analysis of GAPDH mRNA expression in a panel of 72 human tissues. Physiol Genomics 21:389-395.
2. Bergmann M (2006) Physiology of spermatogenesis. In: Andrology for the Clinician. Schill WB, Comhaire FH, Hargreave TB (eds). Springer, Berlin, Heidelberg, pp 272-281.
3. Bustin SA (2000) Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays. J Mol Endocrinol 25:169-193.
4. Cedenho AP, Lima SB, Cenedeze MA, Spaine DM, Ortiz V, Oehninger S (2006) Oligozoospermia and heat-shock protein expression in ejaculated spermatozoa. Hum Reprod 21:1791-1794.
5. Dheda K, Huggett JF, Bustin SA, Johnson MA, Rook G, Zumla A (2004) Validation of housekeeping genes for normalizing RNA expression in real-time PCR. BioTechniques 37:112-119.
6. Dixon WJ (1993) BMDP Statistical Software Manual. University of California Press, Berkeley, Los Angeles, pp 1-3.
7. Ginzinger DG (2002) Gene quantification using real-time quantitative PCR: an emerging technology hits the mainstream. Exp Hematol 30:503-512.
8. Glare EM, Divjak M, Bailey MJ, Walters EH (2002) B-Actin and GAPDH housekeeping gene expression in asthmatic airways is variable and not suitable for normalising mRNA levels. Thorax 57:754-756.
9. Henkel RR, Franken DR, Lombard CJ, Schill WB (1994) Selective capacity of glass-wool filtration for the separation of human spermatozoa with condensed chromatin: a possible therapeutic modality for male-factor cases? J Assist Reprod Genet 11:395-400.
10. Lambard S, Galeraud-Denis I, Bouraïma H, Bourguiba S, Chocat A, Carreau S (2003) Expression of aromatase in human ejaculated spermatozoa: a putative marker of motility. Mol Hum Reprod 9:117-124.
11. Luebbehusen H (2004) The significance of the 260/230 ratio in determining nucleic acid purity. Available at (accessed on 23 June 2004).
12. Miller D (2000) Analysis and significance of messenger RNA in human ejaculated spermatozoa. Mol Reprod Dev 56:259-264.
13. Miller D, Briggs D, Snowden H, Hamlington J, Rollinson S, Lilford R, Krawetz SA (1999) A complex population of RNAs exists in human ejaculate spermatozoa: implications for understanding molecular aspects of spermiogenesis. Gene 237:385-392.
14. Mori R, Wang Q, Danenberg KD, Pinski JK, Danenberg PV (2008) Both B-actin and GAPDH are useful reference genes for normalization of quantitative RT-PCR in human FFPE tissue samples of prostate cancer. Prostate 14:1555-1560.
15. Peddinti D, Nanduri B, Kaya A, Feugang JM, Burgess SC, Memili E (2008) Comprehensive proteomic analysis of bovine spermatozoa of varying fertility rates and identification of biomarkers associated with fertility. BMC Syst Biol 19:1-14.
16. Selvey S, Thompson EW, Matthaei K, Lea RA, Irving MG, Griffiths LR (2001) Beta-actin - an unsuitable internal control for RT-PCR. Mol Cell Probes 15:307-311.
17. Sha J, Zhou Z, Li J, Yin L, Yang H, Hu G, Luo M, Chan HC, Zhou K (2002) Identification of testis development and spermatogenesis related genes in human and mouse testes using cDNA arrays. Mol Hum Reprod 8:511-517.
18. Silver N, Best S, Jiang J, Lay Thein S (2006) Selection of housekeeping genes for gene expression studies in human reticulocytes using real-time PCR. BMC Mol Biol 7:1-9.
19. Steger K, Fink L, Failing K, Bohle RM, Kliesch S, Weidner W, Bergmann M (2003) Decreased protamine-1 transcript levels in testes from infertile men. Mol Hum Reprod 9:331-336.
20. Thellin O, Zorzi W, Lakaye B, De Borman B, Coumans B, Hennen G, Grisar T, Igout A, Heinen E (1999) Housekeeping genes as internal standards: use and limits. J Biotechnol 75:291-295.
21. World Health Organization (1999) WHO Laboratory Manual for the Examination of Human Semen and Sperm-Cervical Mucus Interaction. Cambridge University Press, Cambridge, UK.
22. Wykes MS, Visscher DW, Krawetz SA (1997) Haploid transcripts persist in mature human spermatozoa. Mol Hum Reprod 3:15-19.
23. Wykes SM, Miller D, Krawetz SA (2000) Mammalian spermatozoal mRNAs: tools for the functional analysis of male gametes. Mol Reprod Dev 56:259-264.