Haploid transcripts persist in mature human spermatozoa

Molecular Human Reproduction

Volumn 3, Issue 1, 1997, Pages 15-19

  Wykes, Susan M ^a   Visscher, Daniel W ^b   Krawetz, Stephen A ^a  

^a WAYNE STATE UNIVERSITY SCHOOL OF MEDICINE   (United States)

^b HARPER UNIVERSITY HOSPITAL   (United States)

Author keywords

Haploid transcripts; In situ hybridization; mRNA; Protamines; Spermatogenesis

Indexed keywords

MESSENGER RNA; NONHISTONE PROTEIN; NUCLEAR PROTEIN; PROTAMINE; SPERMATID TRANSITION PROTEINS;

ARTICLE; GENETIC TRANSCRIPTION; GENETICS; HAPLOIDY; HUMAN; IN SITU HYBRIDIZATION; MALE; METABOLISM; SPERMATOZOON;

CHROMOSOMAL PROTEINS, NON-HISTONE; HAPLOIDY; HUMANS; IN SITU HYBRIDIZATION; MALE; NUCLEAR PROTEINS; PROTAMINES; RNA, MESSENGER; SPERMATOZOA; TRANSCRIPTION, GENETIC;

EID: 0030625918     PISSN: 13609947     EISSN: None     Source Type: Journal    
DOI: 10.1093/molehr/3.1.15     Document Type: Article

References (14)

1. Angerer, L. and Angerer, R. (1992) In situ hybridization to cellular RNA with radiolabelled RNA probes. In Wilkinson, D.G. (ed.), In Situ Hybridization. A Practical Approach. Oxford University Press, New York, pp. 16-32.
2. Balhom, R. (1989) Mammalian protamines: structure and molecular interactions. In Aldolph, K.W. (ed.), Molecular Biology of Chromosome Function. Springer, New York, pp. 366-395.
3. Bellvé, A.R., McKav, D J., Renaux, B.S. and Dixon, G.H. (1988) Purification and characterization of mouse protamines P1 and P2. Amino acid sequence of P2. Biochemistry, 27, 2890-2987.
4. Caldwell, K.A. and Handel, M.A. (1991) Protamine transcript sharing among postmeiotic spermatids. Proc. Natl. Acad. Sci. USA, 88, 2407-2411.
5. Chiang, M.H., Main, E.K., Steuerwald, N. et al. (1994) Detection of human leukocyte common antigen class I messenger ribonucleic acid transcripts in human spermatozoa via reverse transcription polymerase chain reaction. Fertil. Steril., 71, 276-280.
6. Clermont, Y. (1963) The cycle of the seminiferous epithelium in man. Am. J. Anat., 112, 35-45.
7. Kumar, G., Patel, D., and Naz, R.K. (1993) c-Myc mRNA is present in human sperm cells. Cell Mol. Biol. Res., 49, 111-117.
8. Miller, D., Tang, P., Skinner, C., and Lilford, R. (1994) Differential RNA fingerprinting as a tool in the analysis of spermatozoal gene expression. Hum. Reprod., 9, 864-869.
9. Nelson, J. E. and Krawetz, S.A. (1993) Linkage of human sperm-specific basic nuclear protein genes. J. Biol. Chem., 268, 2932-2936.
10. Pessot, C.A., Brito, M., Figueroa, J. et al. (1989) The presence of RNA in the sperm nucleus. Biochem. Biophys. Comm., 158, 272-278.
11. Poccia, D. (1989) Reactivation and remodeling of the sperm nucleus following fertilization. In Schatten, H. and Schatten, G. (eds). The Molecular Biology of Fertilization. Academic Press, San Diego, pp. 115-136.
12. 1 integrins in ejaculated human spermatozoa by nested reverse transcription-polymerase chain reaction. Mol. Hum. Reprod., 2, 499-505.
13. Witkin, S.S., Korngold, G.C. and Bendich, A. (1975) Ribonuclease-sensitive DNA synthesizing complex in human sperm heads and seminal fluid. Proc. Natl. Acad. Sci. USA, 75, 3295-3299
14. Wykes, S.M., Nelson, J., Visscher, D.W. et al. (1995) Coordinate expression of the PRM1, PRM2 and TNP2 multigene locus in human testes. DNA Cell Biol., 14, 155-161.