Glia-neuron sandwich cocultures: An in vitro approach to evaluate cell-to-cell communication in neuroinflammation and neurotoxicity

Methods in Molecular Biology

Volumn 758, Issue , 2011, Pages 135-152

  Boraso, Mariaserena ^b   Viviani, Barbara ^a  

^a UNIVERSITY OF MILAN   (Italy)

^b NONE

Author keywords

Biological assay; MTT test; Primary glial cells; Primary hippocampal neurons; Sandwich coculture; TNF

Indexed keywords

TUMOR NECROSIS FACTOR ALPHA;

ANIMAL CELL; ARTICLE; BRAIN NERVE CELL; CELL COMMUNICATION; CELL FUNCTION; CELL INTERACTION; CELL SURVIVAL; COCULTURE; CONTROLLED STUDY; CYTOKINE RELEASE; ENZYME ASSAY; FEMALE; GLIA CELL; HIPPOCAMPUS; NERVE CELL CULTURE; NERVOUS SYSTEM INFLAMMATION; NEUROPATHOLOGY; NEUROTOXICITY; NONHUMAN; PRIORITY JOURNAL; RAT; ANIMAL; ANIMAL EMBRYO; BRAIN; CALIBRATION; CELL LINE; CYTOLOGY; GLIA; INFLAMMATION; METHODOLOGY; NERVE CELL; PATHOLOGY; PREGNANCY; PRIMARY CELL CULTURE; SPRAGUE DAWLEY RAT;

RATTUS;

ANIMALS; BRAIN; CALIBRATION; CELL COMMUNICATION; CELL LINE; CELL SURVIVAL; COCULTURE TECHNIQUES; EMBRYO, MAMMALIAN; FEMALE; INFLAMMATION; NEUROGLIA; NEURONS; PREGNANCY; PRIMARY CELL CULTURE; RATS; RATS, SPRAGUE-DAWLEY; TUMOR NECROSIS FACTOR-ALPHA;

EID: 80052943971     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-61779-170-3_9     Document Type: Article

References (11)

1. Allan, S. M., Rothwell, N. J. (2001) Cytokines and acute neurodegeneration. Nat Rev Neurosci. 10, 734-744.
2. Block M.L., Zecca L., Hong J.S. (2007) Microglia-mediated neurotoxicity: uncovering the molecular mechanisms. Nat. Rev. Neurosci. 8, 57-69.
3. Viviani B., Corsini E., Galli C.L., Padovani A., Ciusani E., Marinovich M. (2000) Dying neural cells activate glia through the release of a protease product. Glia, 32, 84-90, 2000.
4. Biber K., Neumann H., Inoue K., Boddeke H.W.G.M. (2007) Neuronal "on" and "off" signals control microglia. TINS, 30, 596-602.
5. Villa P., Bigini P., Mennini T., Agnello D., Laragione T., Cagnotto A., Viviani B., Marinovich M., Cerami A., Coleman T.R., Brines M., Ghezzi P. (2003) Erythropoietin selectively attenuates cytokine production and inflammation in cerebral ischemia by targeting neuronal apoptosis. J. Exp. Med. 198, 971-975.
6. Vitkovic, L., Bockaert, J., and Jacque, C. (2000). " Inflammatory" cytokines: neuromodulators in normal brain? J Neurochem. 74, 457-71.
7. Viviani B., Gardoni F., Marinovich M. (2007). Cytokines and neuronal ion channels in health and disease. Int. Rev. Neurobiol. 82, 247-63.
8. Srinivasan, D., Yen, J. H., Joseph, D. J., and Friedman, W. (2004). Cell type-specific interleukin-1 beta signaling in the CNS. J Neurosci. 24, 6482-8.
9. Viviani B., Gardoni F., Bartesaghi S., Corsini E., Facchi, Galli CL, DiLuca M., Marinovich M. (2006) IL-1beta released by gp120 drives neural death through tyrosine phosphorylation and trafficking of NMDA receptors J. Biol. Chem.; 281, 30212-30222.
10. Viviani B., Corsini E., Galli C.L., Marinovich M. (1998) Glia increase degeneration of hippocampal neurons through release of tumor necrosis factor-a. Toxicol and Appl. Pharmacol., 150, 271-276.
11. Viviani B., Corsini E., Binaglia M., Galli C.L., Marinovich M. (2001) Reactive oxygen species generated by glia are responsible for neuron death induced by human immunodeficiency virus-glycoprotein 120 in vitro. Neuroscience. 107, 51-58.