In vitro centromere and kinetochore assembly on defined chromatin templates

Nature

Volumn 477, Issue 7364, 2011, Pages 354-358

  Guse, Annika ^a   Carroll, Christopher W ^a   Moree, Ben ^a   Fuller, Colin J ^a   Straight, Aaron F ^a  

^a STANFORD UNIVERSITY SCHOOL OF MEDICINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CENTROMERE PROTEIN A; HISTONE;

BIOCHEMISTRY; CHEMICAL BINDING; CHROMOSOME; CYTOLOGY; DNA; EUKARYOTE; FUNCTIONAL ROLE; PROTEIN; SUBSTRATE;

ARTICLE; CARBOXY TERMINAL SEQUENCE; CENTROMERE; CHROMATIN; CHROMOSOME; IN VITRO STUDY; MICROTUBULE; MITOSIS; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; AUTOANTIGENS; CELL EXTRACTS; CELL-FREE SYSTEM; CENTROMERE; CHROMATIN; CHROMOSOMAL PROTEINS, NON-HISTONE; CHROMOSOME SEGREGATION; CONSERVED SEQUENCE; HISTONES; HUMANS; KINETOCHORES; MICROTUBULES; MITOSIS; OOCYTES; PROTEIN STRUCTURE, TERTIARY; TEMPLATES, GENETIC; XENOPUS LAEVIS;

CHIMAERIFORMES; EUKARYOTA;

EID: 80052849224     PISSN: 00280836     EISSN: 14764687     Source Type: Journal    
DOI: 10.1038/nature10379     Document Type: Article

References (37)

1. Cheeseman, I. M. & Desai, A. Molecular architecture of the kinetochoremicrotubule interface. Nature Rev. Mol. Cell Biol. 9, 33-46 (2008).
2. Black, B. E. et al. Structural determinants for generating centromeric chromatin. Nature 430, 578-582 (2004). (Pubitemid 38998633)
3. Black, B. E. & Bassett, E. A. The histone variant CENP-A and centromere specification. Curr. Opin. Cell Biol. 20, 91-100 (2008).
4. Sekulic, N., Bassett, E. A., Rogers, D. J. & Black, B. E. The structure of (CENP-A-H4)2 reveals physical features that mark centromeres. Nature 467, 347-351 (2010).
5. Carroll, C. W., Milks, K. J. & Straight, A. F. Dual recognition of CENP-A nucleosomes is required for centromere assembly. J. Cell Biol. 189, 1143-1155 (2010).
6. Carroll, C. W., Silva, M. C. C., Godek, K. M., Jansen, L. E. T.&Straight,A. F.Centromere assembly requires the direct recognition of CENP-A nucleosomes by CENP-N. Nature Cell Biol. 11, 896-902 (2009).
7. Dunleavy, E. M. et al.HJURPis a cell-cycle-dependentmaintenance anddeposition factor of CENP-A at centromeres. Cell 137, 485-497 (2009).
8. Foltz, D. R. et al. Centromere-specific assembly of CENP-A nucleosomes is mediated by HJURP. Cell 137, 472-484 (2009).
9. Hu, H. et al. Structure of a CENP-A-histone H4 heterodimer in complex with chaperone HJURP. Genes Dev. 25, 901-906 (2011).
10. Tachiwana, H. et al. Crystal structure of the human centromeric nucleosome containing CENP-A. Nature. doi:10.1038/nature10258 (10 July, 2011).
11. Blower, M. D., Sullivan, B. A.&Karpen, G. H. Conserved organization of centromeric chromatin in flies and humans. Dev. Cell 2, 319-330 (2002). (Pubitemid 34266117)
12. Ribeiro, S. et al. A super-resolution map of the vertebrate kinetochore. Proc. Natl Acad. Sci. USA 107, 10484-10489 (2010).
13. Zinkowski, R. P.,Meyne, J.&Brinkley, B. R. The centromere-kinetochore complex: a repeat subunit model. J. Cell Biol. 113, 1091-1110 (1991). (Pubitemid 21909741)
14. Huynh, V., Robinson, P. & Rhodes, D. A method for the in vitro reconstitution of a defined "30 nm" chromatin fibre containing stoichiometric amounts of the linker histone. J. Mol. Biol. 345, 957-968 (2005). (Pubitemid 40092217)
15. Luger, K., Rechsteiner, T. J., Flaus, A. J., Waye, M. M. & Richmond, T. J. Characterization of nucleosome core particles containing histone proteins made in bacteria. J. Mol. Biol. 272, 301-311 (1997). (Pubitemid 27410045)
16. Desai, A., Murray, A., Mitchison, T. J. & Walczak, C. E. The use of Xenopus egg extracts to study mitotic spindle assembly and function in vitro. Methods Cell Biol. 61, 385-412 (1999).
17. Hori, T. et al. CCAN makes multiple contacts with centromeric DNA to provide distinct pathways to the outer kinetochore. Cell 135, 1039-1052 (2008).
18. Kawashima, S. A., Yamagishi, Y., Honda, T., Ishiguro, K.-i. & Watanabe, Y. Phosphorylation of H2A by Bub1 prevents chromosomal instability through localizing shugoshin. Science 327, 172-177 (2010).
19. Kelly, A. E. et al. Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B. Science 330, 235-239 (2010).
20. Wang, F. et al. Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis. Science 330, 231-235 (2010).
21. Budde, P. P., Kumagai, A., Dunphy, W. G. & Heald, R. Regulation of Op18 during spindle assembly in Xenopus egg extracts. J. Cell Biol. 153, 149-158 (2001).
22. Blow, J. J.& Laskey, R. A. Initiation ofDNA replication in nuclei and purifiedDNAby a cell-free extract of Xenopus eggs. Cell 47, 577-587 (1986). (Pubitemid 17215347)
23. Minshull, J., Sun, H., Tonks, N. K. & Murray, A. W. A MAP kinase-dependent spindle assembly checkpoint in Xenopus egg extracts. Cell 79, 475-486 (1994). (Pubitemid 24346062)
24. Sawin, K. E.&Mitchison, T. J. Mitotic spindle assembly by two different pathways in vitro. J. Cell Biol. 112, 925-940 (1991). (Pubitemid 21909639)
25. Heald, R. et al. Self-organization of microtubules into bipolar spindles around artificial chromosomes in Xenopus egg extracts. Nature 382, 420-425 (1996). (Pubitemid 26264851)
26. Nicklas, R. B., Ward, S. C. & Gorbsky, G. J. Kinetochore chemistry is sensitive to tension and may link mitotic forces to a cell cycle checkpoint. J. Cell Biol. 130, 929-939 (1995).
27. Rieder, C. L., Cole, R. W., Khodjakov, A. & Sluder, G. The checkpoint delaying anaphase in response to chromosome monoorientation is mediated by an inhibitory signal produced by unattached kinetochores. J. Cell Biol. 130, 941-948 (1995).
28. Milks, K. J., Moree, B. & Straight, A. F. Dissection of CENP-C-directed centromere and kinetochore assembly. Mol. Biol. Cell 20, 4246-4255 (2009).
29. Foltz, D. R. et al. The human CENP-A centromeric nucleosome-associated complex. Nature Cell Biol. 8, 458-469 (2006).
30. McClelland, S. E. et al. The CENP-A NAC/CAD kinetochore complex controls chromosomecongression and spindle bipolarity.EMBOJ.26, 5033-5047 (2007). (Pubitemid 350261290)
31. Luger, K., Rechsteiner, T. & Richmond, T. Preparation of nucleosome core particle from recombinant histones. Methods Enzymol. 304, 3-19 (1999). (Pubitemid 29268874)
32. Lowary, P. T. & Widom, J. New DNA sequence rules for high affinity binding to histone octamer and sequence-directed nucleosome positioning. J. Mol. Biol. 276, 19-42 (1998). (Pubitemid 28085408)
33. Murray, A. W. Cell cycle extracts. Methods Cell Biol. 36, 581-605 (1991).
34. Kim, S., Song, E., Lee, K. & Ferrell, J. Jr. Multisite M-phase phosphorylation of Xenopus Wee1A. Mol. Cell. Biol. 25, 10580-10590 (2005). (Pubitemid 41681978)
35. Hannak, E. & Heald, R. Investigating mitotic spindle assembly and function in vitro using Xenopus laevis egg extracts. Nature Protocols 1, 2305-2314 (2006).
36. Field, C. M., Oegema, K., Zheng, Y., Mitchison, T. J. & Walczak, C. E. Purification of cytoskeletal proteins using peptide antibodies. Methods Enzymol. 298, 525-541 (1998). (Pubitemid 28450777)
37. Osborne, J. W. Best Practices in Quantitative Methods (Sage Publications, 2008).