Tyrosine-targeted spin labeling and EPR spectroscopy: An alternative strategy for studying structural transitions in proteins

Angewandte Chemie - International Edition

Volumn 50, Issue 39, 2011, Pages 9108-9111

  Lorenzi, Magali ^a,b   Puppo, Carine ^a,b   Lebrun, Régine ^b   Lignon, Sabrina ^b   Roubaud, Valérie ^c   Martinho, Marlène ^a,b   Mileo, Elisabetta ^c   Tordo, Paul ^c   Marque, Sylvain R A ^c   Gontero, Brigitte ^a,b   Guigliarelli, Bruno ^a,b   Belle, Valérie ^a,b  

^a CNRS   (France)

^b CNRS and Aix Marseille University   (France)

^c AIX MARSEILLE UNIVERSITY   (France)

Author keywords

bioconjugation; EPR spectroscopy; Mannich reaction; proteins; radicals

Indexed keywords

BIO-CONJUGATION; COMPLEX FORMATIONS; EPR SPECTROSCOPY; HIGH MOBILITY; LABELED PROTEINS; MANNICH REACTIONS; MANNICH-TYPE REACTIONS; RADICALS; SITE-DIRECTED SPIN LABELING; SPIN LABELING; STRUCTURAL TRANSITIONS; THREE-COMPONENT; TYROSINE RESIDUES;

AMINO ACIDS; MASS SPECTROMETRY; PARAMAGNETIC RESONANCE; PROTEINS;

ELECTRON SPIN RESONANCE SPECTROSCOPY;

CHLOROPLAST PROTEIN; GLYCERALDEHYDE 3 PHOSPHATE DEHYDROGENASE; MANNICH BASE; PROTEIN; TYROSINE;

ARTICLE; CHEMISTRY; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE; GREEN ALGA; MASS SPECTROMETRY; METABOLISM; METHODOLOGY; SPIN LABELING;

CHLOROPHYTA; CHLOROPLAST PROTEINS; CIRCULAR DICHROISM; ELECTRON SPIN RESONANCE SPECTROSCOPY; GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASES; MANNICH BASES; PROTEINS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION; SPIN LABELS; TYROSINE;

EID: 80052829953     PISSN: 14337851     EISSN: 15213773     Source Type: Journal    
DOI: 10.1002/anie.201102539     Document Type: Article

References (22)

1. W. L. Hubbell, D. S. Cafiso, C. Altenbach, Nat. Struct. Biol. 2000, 7, 735-739
2. J. P. Klare, H. J. Steinhoff, Photosynth. Res. 2009, 102, 377-390
3. V. Belle, S. Rouger, S. Costanzo, S. Longhi, A. Fournel, in Assessing Structures and Conformations of Intrinsically Disordered Proteins (Ed.:, V. N. Uversky,), Wiley, Hoboken, 2009.
4. B. Morin, J. M. Bourhis, V. Belle, M. Woudstra, F. Carriere, B. Guigliarelli, A. Fournel, S. Longhi, J. Phys. Chem. B 2006, 110, 20596-20608
5. N. L. Pirman, E. Milshteyn, L. Galiano, J. C. Hewlett, G. E. Fanucci, Protein Sci. 2011, 20, 150-159.
6. C. Kumsta, U. Jakob, Biochemistry 2009, 48, 4666-4676.
7. J. Erales, M. Lorenzi, R. Lebrun, A. Fournel, E. Etienne, C. Courcelle, B. Guigliarelli, B. Gontero, V. Belle, Biochemistry 2009, 48, 6034-6040.
8. M. R. Fleissner, E. M. Brustad, T. Kalai, C. Altenbach, D. Cascio, F. B. Peters, K. Hideg, S. Peuker, P. G. Schultz, W. L. Hubbell, Proc. Natl. Acad. Sci. USA 2009, 106, 21637-21642.
9. M. D. Best, Biochemistry 2009, 48, 6571-6584.
10. J. M. Antos, M. B. Francis, J. Am. Chem. Soc. 2004, 126, 10256-10257.
11. J. M. McFarland, N. S. Joshi, M. B. Francis, J. Am. Chem. Soc. 2008, 130, 7639-7644
12. H. Ban, J. Gavrilyuk, C. F. Barbas, J. Am. Chem. Soc. 2010, 132, 1523-1525.
13. F. Gardebien, R. R. Thangudu, B. Gontero, B. Offmann, J. Mol. Graphics Modell. 2006, 25, 186-195.
14. M. M. Bradford, Anal. Biochem. 1976, 72, 248-254.
15. E. Graciet, P. Gans, N. Wedel, S. Lebreton, J. M. Camadro, B. Gontero, Biochemistry 2003, 42, 8163-8170.
16. J. Erales, L. Avilan, S. Lebreton, B. Gontero, FEBS J. 2008, 275, 1248-1259.
17. K. B. Qu, J. L. Vaughn, A. Sienkiewicz, C. P. Scholes, J. S. Fetrow, Biochemistry 1997, 36, 2884-2897.
18. H. S. Mchaourab, M. A. Lietzow, K. Hideg, W. L. Hubbell, Biochemistry 1996, 35, 7692-7704
19. V. P. Timofeev, V. I. Tsetlin, Biophys. Struct. Mech. 1983, 10, 93-108.
20. S. Popp, L. Packschies, N. Radzwill, K. P. Vogel, H. A. Steinhoff, J. Reinstein, J. Mol. Biol. 2005, 347, 1039-1052.
21. V. Belle, S. Rouger, S. Costanzo, E. Liquière, J. Strancar, B. Guigliarelli, A. Fournel, S. Longhi, Proteins 2008, 73, 973-988.
22. S. Lebreton, S. Andreescu, E. Graciet, B. Gontero, FEBS J. 2006, 273, 3358-3369.