Characterization of a three-component coupling reaction on proteins by isotopic labeling and nuclear magnetic resonance spectroscopy

Journal of the American Chemical Society

Volumn 130, Issue 24, 2008, Pages 7639-7644

  McFarland, Jesse M ^a   Joshi, Neel S ^a   Francis, Matthew B ^a  

^a NONE

Author keywords

[No Author keywords available]

Indexed keywords

ADDITION REACTIONS; ALDEHYDES; AMINO ACIDS; AROMATIC COMPOUNDS; ATOMS; BIOMOLECULES; CHEMICAL BONDS; CHEMICAL REACTIONS; COMPUTER NETWORKS; FORMALDEHYDE; ISOTOPES; LABELING; MAGNETIC FIELDS; MAGNETIC RESONANCE; MAGNETISM; NUCLEAR MAGNETIC RESONANCE; NUCLEAR MAGNETIC RESONANCE SPECTROSCOPY; NUMBER THEORY; NUMERICAL METHODS; ORGANIC COMPOUNDS; PHENOLS; PROTEINS; RESONANCE; STEREOSELECTIVITY; SUBSTITUTION REACTIONS;

AMERICAN CHEMICAL SOCIETY (ACS); ANALYSIS METHODS; BIO-CONJUGATION; CYSTEINE RESIDUES; DETAILED STUDY; ELECTROPHILIC AROMATIC SUBSTITUTIONS; INDOLE RINGS; ISOTOPIC LABELLING; ISOTOPICALLY ENRICHED; MAGNETIC (CE); MILD CONDITIONS; REACTION PRODUCTS; RESONANCE SPECTROSCOPY; SIDE CHAINS; STRUCTURAL CONFIRMATION; THREE COMPONENT COUPLING; THREE-COMPONENT; TRYPTOPHAN RESIDUES; TYROSINE (TYR); TYROSINE RESIDUES;

REACTION KINETICS;

ACETAL DERIVATIVE; CYSTEINE; INDOLE; PROTEIN; TRYPTOPHAN; TYROSINE;

ARTICLE; CHEMICAL REACTION; CONJUGATION; CROSS COUPLING REACTION; DISULFIDE BOND; ISOTOPE LABELING; NUCLEAR MAGNETIC RESONANCE; PROTEIN ANALYSIS; PROTEIN MODIFICATION; PROTEIN STRUCTURE; REDUCTION; STRUCTURE ANALYSIS;

CARBON RADIOISOTOPES; CHYMOTRYPSINOGEN; CYSTEINE; FORMALDEHYDE; HUMANS; ISOTOPE LABELING; MURAMIDASE; NUCLEAR MAGNETIC RESONANCE, BIOMOLECULAR; PAPAIN; PROTEIN CONFORMATION; PROTEINS; THIOREDOXINS; TRYPTOPHAN; TYROSINE;

EID: 45249106551     PISSN: 00027863     EISSN: None     Source Type: Journal    
DOI: 10.1021/ja710927q     Document Type: Article

References (21)

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14. Two additional peaks appear at 32 ppm in F1 at 2.6 and 1.9 ppm, respectively. These peaks are much lower in intensity and do not correlate well to any model structures. Thus, they could not be assigned in this study (see Figure S4).
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