Δ 6-Desaturase from Mortierella alpina: cDNA cloning, expression, and phylogenetic analysis

Biotechnology Letters

Volumn 33, Issue 10, 2011, Pages 1985-1991

  Liu, Jianmin ^a,b   Li, Dianrong ^a,c   Yin, Yongtai ^a   Wang, Hao ^c   Li, Maoteng ^a   Yu, Longjiang ^a  

^a HUAZHONG UNIVERSITY OF SCIENCE AND TECHNOLOGY   (China)

^b HENAN POLYTECHNIC UNIVERSITY   (China)

^c Hybrid Rapeseed Research Center of Shaanxi Province   (China)

Author keywords

6 fatty acid desaturase; Linolenic acid; Cloning and expression; Mortierella alpina; Phylogenetic analysis

Indexed keywords

CLONING AND EXPRESSION; DESATURASE; LINOLENIC ACIDS; MORTIERELLA ALPINA; PHYLOGENETIC ANALYSIS;

CLONING; GENES; METHANOL; UNSATURATED FATTY ACIDS;

LINOLEIC ACID;

COMPLEMENTARY DNA; FATTY ACID; FUNGAL RNA; GAMMA LINOLENIC ACID; LINOLEOYL COENZYME A DESATURASE; MESSENGER RNA; RECOMBINANT PROTEIN;

ARTICLE; BIOSYNTHESIS; CHEMISTRY; DNA SEQUENCE; ENZYMOLOGY; GENETICS; ISOLATION AND PURIFICATION; MASS FRAGMENTOGRAPHY; METABOLISM; METHODOLOGY; MOLECULAR CLONING; MORTIERELLA; PHYLOGENY; PICHIA; SEQUENCE ALIGNMENT;

CLONING, MOLECULAR; DNA, COMPLEMENTARY; FATTY ACIDS; GAMMA-LINOLENIC ACID; GAS CHROMATOGRAPHY-MASS SPECTROMETRY; LINOLEOYL-COA DESATURASE; MORTIERELLA; PHYLOGENY; PICHIA; RECOMBINANT PROTEINS; RNA, FUNGAL; RNA, MESSENGER; SEQUENCE ALIGNMENT; SEQUENCE ANALYSIS, DNA;

MORTIERELLA ALPINA; PICHIA PASTORIS;

EID: 80052809961     PISSN: 01415492     EISSN: 15736776     Source Type: Journal    
DOI: 10.1007/s10529-011-0650-4     Document Type: Article

References (16)

1. Cereghino JL, Cregg JM (2000) Heterologous protein expression in the methylotrophic yeast Pichia pastoris. FEMS Microbiol Rev 24: 45-66.
2. Gunstone FD (1992) Gamma linolenic acid-occurrence and physical and chemical properties. Prog Lipid Res 31: 145-161.
3. 6 desaturases from Mortierella alpina and recombinant production of γ-linolenic acid in Saccharomyces cerevisiae. Lipids 34: 649-659.
4. Kremer JM (1996) Effects of modulation of inflammatory and immune parameters in patients with rheumatic and inflammatory disease receiving dietary supplementation of n-3 and n-6 fatty acids. Lipids 31: 243-247.
5. Laoteng K, Mannontarat R, Tanticharoen M et al (2000) Delta-6-desaturase of Mucor rouxii with high similarity to plant delta-6-desaturase and its heterologous expression in Saccharomyces cerevisiae. Biochem Biophys Res Commun 279: 17-22.
6. Li YT, Li MT, Fu CH et al (2009) Improvement of arachidonic acid and eicosapentaenoic acid production by increasing the copy number of the genes encoding fatty acid desaturase and elongase into Pichia pastoris. Biotechnol Lett 31: 1011-1017.
7. Liu L, Li MC, Hu GW et al (2001a) Expression of delta 6 fatty acid desaturase gene from Mortierella alpina in Saccharomyces cerevisiae. Chin J Biotechnol 17: 161-164.
8. Liu L, Li MC, Hu GW et al (2001b) Identification of Mortierella isabellina M6-22 delta 6 fatty acid desaturase by heterologous expression in Saccharomyces cerevisiae. Acta Microbiol Sin 41: 397-401.
9. Na-Ranong S, Laoteng K, KIttakoop P et al (2006) Targeted mutagenesis of a fatty acid Delta (6)-desaturase from Mucor rouxii: role of amino acid residues adjacent to histidine-rich motif II. Biochem Biophys Res Commun 339: 1029-1034.
10. 6 desaturase gene from the cyanobacterium Synechocystis sp. strain PCC6803 by gain-of-function expression in Anabaena sp. strain PCC7120. Plant Mol Biol 22: 293-300.
11. Sakuradani E, Kohayashi M, Shimizu S (1999) Δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus gene cloning and its heterologous expression in a fungus, Aspergillus. Gene 238: 445-453.
12. Tamura K, Dudley J, Nei M et al (2007) MEGA4: molecular evolutionary genetics analysis (MEGA) software version 4. 0. Mol Biol Evol 24: 1596-1599.
13. Tocher DR, Leaver MJ, Hodgson PA (1998) Recent advances in the biochemistry and molecular biology of fatty acyl desaturases. Prog Lipid Res 37: 73-117.
14. 6-fatty acid desaturase gene from Rhizopus arrhizus. FEBS Lett 556: 81-85.
15. Zhang Q, Miao CP, Li MI, Li SL (2006) A phylogenetic analysis of Δ6-fatty acid desaturases. J Yunnan Univ 28: 450-455.
16. Zhu M, Zhou PP, Yu LJ (2002) Extraction of lipids from Mortierella alpina and enrichment of arachidonic acid from the fungal lipids. Bioresour Technol 84: 93-95.