Pulsed-field gel electrophoresis and pcr characterization of environmental Vibrio parahaemolyticus strains of different origins

Applied and Environmental Microbiology

Volumn 77, Issue 17, 2011, Pages 6301-6304

  Suffredini, E ^a   Lopez Joven, C ^b,c   Maddalena, L ^a   Croci, L ^a   Roque, A ^b  

^a ISTITUTO SUPERIORE DI SANITÀ   (Italy)

^b IRTA   (Spain)

^c UNIVERSIDAD DE ZARAGOZA   (Spain)

Author keywords

[No Author keywords available]

Indexed keywords

DIFFERENT ORIGINS; ENVIRONMENTAL ISOLATES; EUROPEAN COUNTRIES; GENETIC RELATIONSHIPS; PULSED FIELD GEL ELECTROPHORESIS; V. PARAHAEMOLYTICUS; VIBRIO PARAHAEMOLYTICUS;

GELS;

ELECTROPHORESIS;

BACTERIUM; ELECTROKINESIS; EXPERIMENTAL STUDY; GENETIC VARIATION; INTRASPECIFIC VARIATION;

ARTICLE; CLASSIFICATION; EUROPE; GENETIC VARIABILITY; GENETICS; ISOLATION AND PURIFICATION; MICROBIOLOGY; MOLECULAR TYPING; POLYMERASE CHAIN REACTION; PULSED FIELD GEL ELECTROPHORESIS; VIBRIO PARAHAEMOLYTICUS;

ELECTROPHORESIS, GEL, PULSED-FIELD; ENVIRONMENTAL MICROBIOLOGY; EUROPE; GENETIC VARIATION; MOLECULAR TYPING; POLYMERASE CHAIN REACTION; VIBRIO PARAHAEMOLYTICUS;

EUROPE;

VIBRIO PARAHAEMOLYTICUS;

EID: 80052731567     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00333-11     Document Type: Article

References (26)

1. Baffone, W., et al. 2006. Detection of free-living and plankton-bound vibrios in coastal waters of the Adriatic Sea (Italy) and study of their pathogenicity-associated properties. Environ. Microbiol. 8:1299-1305.
2. Baker-Austin, C., L. Stockley, R. Rangdale, and J. Martinez-Urtaza. 2010. Environmental occurrence and clinical impact of Vibrio vulnificus and Vibrio parahaemolyticus: a European perspective. Environ. Microbiol. Rep. 2:7-18.
3. Bauer, A., Y. Ostensvik, M. Florvag, O. Ormen, and L. M. Rorvik. 2006. Occurrence of Vibrio parahaemolyticus, V. cholerae, and V. vulnificus in Norwegian blue mussels (Mytilus edulis). Appl. Environ. Microbiol. 72:3058-3061.
4. Bej, A. K., et al. 1999. Detection of total and hemolysin-producing Vibrio parahaemolyticus in shellfish using multiplex PCR amplification of tl, tdh and trh. J. Microbiol. Methods 36:215-225.
5. Caburlotto, G., V. Ghidini, M. Gennari, M. Tafi, and M. M. Lleo. 2008. Isolation of a Vibrio parahaemolyticus pandemic strain from a marine water sample obtained in the northern Adriatic. Euro Surveill. 13:8068.
6. Croci, L., et al. 2007. Evaluation of different PCR methods for the identification of Vibrio parahaemolyticus strains isolated by cultural methods. J. AOAC Int. 90:1588-1597.
7. Dryselius, R., K. Kurokawa, and T. Iida. 2007. Vibrionaceae, a versatile bacterial family with evolutionarily conserved variability. Res. Microbiol. 158:479-486.
8. Ellingsen, A. B., H. Jorgensen, S. Wagley, M. Monshaugen, and L. M. Rorvik. 2008. Genetic diversity among Norwegian Vibrio parahaemolyticus. J. Appl. Microbiol. 105:2195-2202.
9. Hervio-Heath, D., et al. 2002. Occurrence of pathogenic vibrios in coastal areas of France. J. Appl. Microbiol. 92:1123-1135.
10. Lizarraga-Partida, M. L., and M. L. Quilici. 2009. Molecular analyses of Vibrio cholerae O1 clinical strains, including new nontoxigenic variants isolated in Mexico during the cholera epidemic years between 1991 and 2000. J. Clin. Microbiol. 47:1364-1371.
11. Martinez-Urtaza, J., et al. 2004. Characterization of pathogenic Vibrio parahaemolyticus isolates from clinical sources in Spain and comparison with Asian and North American pandemic isolates. J. Clin. Microbiol. 42:4672-4678.
12. Martinez-Urtaza, J., et al. 2008. Environmental determinants of the occurrence and distribution of Vibrio parahaemolyticus in the rias of Galicia, Spain. Appl. Environ. Microbiol. 74:265-274.
13. Montilla, R., J. Palomar, M. Santmarti, C. Fuste, and M. Vinas. 1994. Isolation and characterization of halophilic Vibrio from bivalves bred in nurseries at the Ebre delta. J. Invertebr. Pathol. 63:178-181.
14. Ottaviani, D., et al. 2010. Vibrio parahaemolyticus-associated gastroenteritis in Italy: persistent occurrence of O3:K6 pandemic clone and emergence of O1:KUT serotype. Diagn. Microbiol. Infect. Dis. 66:452-455.
15. Ripabelli, G., G. M. Grasso, M. L. Sammarco, and I. Luzzi. 1997. Procedure di isolamento e caratterizzazione di Vibrio spp. di importanza clinica. Rapporti ISTISAN. 31:1-55.
16. Robert-Pillot, A., et al. 2004. Occurrence of the tdh and trh genes in Vibrio parahaemolyticus isolates from waters and raw shellfish collected in two French coastal areas and from seafood imported into France. Int. J. Food Microbiol. 91:319-325.
17. Roche Applied Science. 25 November 2009, posting date. DIG application manual for filter hybridization. Roche Applied Science, Indianapolis, IN. http://www.roche-applied-science.com/PROD_INF/MANUALS/DIG_MAN/dig_toc.htm.
18. Rodriguez-Castro, A., et al. 2010. Prevalence and genetic diversity of pathogenic populations of Vibrio parahaemolyticus in coastal waters of Galicia, Spain. Environ. Microbiol. Rep. 2:58-66.
19. Roque, A., et al. 2009. Detection and identification of tdh- and trh-positive Vibrio parahaemolyticus strains from four species of cultured bivalve molluscs on the Spanish Mediterranean Coast. Appl. Environ. Microbiol. 75:7574-7577.
20. Suffredini, E., L. Cozzi, and L. Croci. 2009. Colony hybridization method for the simultaneous enumeration of total and pathogenic V. parahaemolyticus in shellfish, p. 206. In Abstr. 7th Int. Conf. Molluscan Shellfish Safety (IC-MSS09), Nantes, France.
21. Tada, J., et al. 1992. Detection of the thermostable direct hemolysin gene (tdh) and the thermostable direct hemolysin-related hemolysin gene (trh) of Vibrio parahaemolyticus by PCR. Mol. Cell. Probes 6:477-487.
22. Tanil, G. B., et al. 2005. Characterization of Vibrio parahaemolyticus isolated from coastal seawater in peninsular Malaysia. Southeast Asian J. Trop. Med. Public Health 36:940-945.
23. Wagley, S., K. Koofhethile, and R. Rangdale. 2009. Prevalence and potential pathogenicity of Vibrio parahaemolyticus in Chinese mitten crabs (Eriocheir sinensis) harvested from the River Thames estuary, England. J. Food Prot. 72:60-66.
24. Wagley, S., K. Koofhethile, J. B. Wing, and R. Rangdale. 2008. Comparison of V. parahaemolyticus isolated from seafoods and cases of gastrointestinal disease in the UK. Int. J. Environ. Health Res. 18:283-293.
25. Wang, Y. W., L. L. Chern, P. D. Cam, and C. S. Chiou. 2008. Evaluation of restriction enzymes for standardizing pulsed-field gel electrophoresis proto- col for rapid subtyping of Vibrio parahaemolyticus. Diagn. Microbiol. Infect. Dis. 61:251-255.
26. Wong, H. C., et al. 2007. A pulsed-field gel electrophoresis typing scheme for Vibrio parahaemolyticus isolates from fifteen countries. Int. J. Food Micro-biol. 114:280-287.