Detailed protocol for evaluation of dynamic perifusion of human islets to assess β-cell function

Islets

Volumn 3, Issue 5, 2011, Pages 284-290

  Bentsi Barnes, Kwamina ^a   Doyle, Máire E ^b   Abad, Danny ^a   Kandeel, Fouad ^a   Al Abdullah, Ismail H ^a  

^a City of Hope ^*  (United States)

^b JOHNS HOPKINS BAYVIEW MEDICAL CENTER   (United States)

Author keywords

Exendin 4; Glucose; Human islets of Langerhans; Insulin release

Indexed keywords

EXENDIN 4; GHRELIN RECEPTOR; GLUCOSE;

ARTICLE; CLINICAL ASSESSMENT; CLINICAL EVALUATION; CLINICAL EXAMINATION; CONTROLLED STUDY; COST CONTROL; GLUCOSE BLOOD LEVEL; HORMONE RELEASE; HUMAN; IN VIVO STUDY; INSULIN RELEASE; LABORATORY TEST; METHODOLOGY; PANCREAS ISLET BETA CELL; PANCREAS ISLET CELL FUNCTION;

ADULT; CADAVER; EQUIPMENT DESIGN; GLUCOSE; HUMANS; INSULIN; INSULIN-SECRETING CELLS; ISLETS OF LANGERHANS TRANSPLANTATION; MIDDLE AGED; PERFUSION; POTASSIUM CHLORIDE; REPRODUCIBILITY OF RESULTS; TISSUE DONORS;

EID: 80052716132     PISSN: 19382014     EISSN: 19382022     Source Type: Journal    
DOI: 10.4161/isl.3.5.15938     Document Type: Article

References (39)

1. Shapiro AM, Lakey JR, Ryan EA, Korbutt GS, Toth E, Warnock GL, et al. Islet transplantation in seven patients with type 1 diabetes mellitus using a glucocorticoid-free immunosuppressive regimen. N Engl J Med 2000; 343:230-8; PMID:10911004; DOI:10.1056/NEJM200007273430401.
2. Shapiro AM, Ricordi C, Hering BJ, Auchincloss H, Lindblad R, Robertson RP, et al. International trial of the Edmonton protocol for islet transplantation. N Engl J Med 2006; 355:1318-30; PMID:17005949; DOI:10.1056/NEJMoa061267.
3. Ricordi C, Lacy PE, Scharp DW. Automated islet isolation from human pancreas. Diabetes 1989; 38:140-2; PMID:2642838.
4. Antonioli B, Fermo I, Cainarca S, Marzorati S, Nano R, Baldissera M, et al. Characterization of collagenase blend enzymes for human islet transplantation. Transplantation 2007; 84:1568-75; PMID:18165766; DOI:10.1097/01.tp.0000295719.88525.60.
5. Faradji RN, Monroy K, Messinger S, Pileggi A, Froud T, Baidal DA, et al. Simple measures to monitor beta-cell mass and assess islet graft dysfunction. Am J Transplant 2007; 7:303-8; PMID:17173660; DOI:10.1111/j.1600-6143.2006.01620. x.
6. Pileggi A, Alejandro R, Ricordi C. Clinical islet transplantation. Minerva Endocrinol 2006; 31:219-32; PMID:17213789.
7. Hering BJ, Kandaswamy R, Ansite JD, Eckman PM, Nakano M, Sawada T, et al. Single-donor, marginal-dose islet transplantation in patients with type 1 diabetes. JAMA 2005; 293:830-5; PMID:15713772; DOI:10.1001/jama.293.7.830.
8. Tan J, Yang S, Cai J, Guo J, Huang L, Wu Z, et al. Simultaneous islet-kidney transplantation in 7 patients of type 1 diabetes with end-stage renal disease using a glucocorticoid-free immunosuppressive regimen with alemtuzumab induction. Diabetes 2008; 57:2666-71; PMID:18633105; DOI:10.2337/db08-0358.
9. Ashcroft SJ, Bassett JM, Randle PJ. Isolation of human pancreatic islets capable of releasing insulin and metabolising glucose in vitro. Lancet 1971; 1:888-9; PMID:4102029; DOI:10.1016/S0140-6736(71)92445-7.
10. Hopcroft DW, Mason DR, Scott RS. Standardization of insulin secretion from pancreatic islets: validation of a DNA assay. Horm Metab Res 1985; 17:559-61; PMID:3908266; DOI:10.1055/s-2007-1013606.
11. Norfleet WT, Pagliara AS, Haymond MW, Matschinsky F. Comparison of alpha- and beta-cell secretory responses in islets isolated with collagenase and in the isolated perfused pancreas of rats. Diabetes 1975; 24:961-70; PMID:171190; DOI:10.2337/diabetes.24.11.961.
12. Dufrane D, Nenquin M, Henquin JC. Nutrient control of insulin secretion in perifused adult pig islets. Diabetes Metab 2007; 33:430-8; PMID:17584514; DOI:10.1016/j.diabet.2007.05.001.
13. Haber EP, Hirabara SM, Gomes AD, Curi R, Carpinelli AR, Carvalho CR. Palmitate modulates the early steps of insulin signalling pathway in pancreatic islets. FEBS Lett 2003; 544:185-8; PMID:12782313; DOI:10.1016/S0014-5793(03) 00503-9.
14. Henquin JC, Dufrane D, Nenquin M. Nutrient control of insulin secretion in isolated normal human islets. Diabetes 2006; 55:3470-7; PMID:17130494; DOI:10.2337/db06-0868.
15. Szkudelski T. The insulin-suppressive effect of resveratrol - an in vitro and in vivo phenomenon. Life Sci 2008; 82:430-5; PMID:18206958; DOI:10.1016/j.lfs.2007.12.008.
16. Zawalich WS, Tesz GJ, Yamazaki H, Zawalich KC, Philbrick W. Dexamethasone suppresses phospholipase C activation and insulin secretion from isolated rat islets. Metabolism 2006; 55:35-42; PMID:16324917; DOI:10.1016/j.metabol.2005.06. 023.
17. Zawalich WS, Zawalich KC. Effects of protein kinase C inhibitors on insulin secretory responses from rodent pancreatic islets. Mol Cell Endocrinol 2001; 177:95-105; PMID:11377825; DOI:10.1016/S0303-7207(01)00422-1.
18. Cabrera O, Jacques-Silva MC, Berman DM, Fachado A, Echeverri F, Poo R, et al. Automated, high-throughput assays for evaluation of human pancreatic islet function. Cell Transplant 2008; 16:1039-48; PMID:18351020; DOI:10.3727/ 000000007783472408.
19. Nduaguibe CC, Bentsi-Barnes K, Mullen Y, Kandeel F, Al-Abdullah IH. Serine protease inhibitors suppress pancreatic endogenous proteases and modulate bacterial neutral proteases. Islets 2010; 2:200-6; PMID:21099314; DOI:10.4161/isl.2.3.11714.
20. Bentsi-Barnes K, Kandeel F, Al-Abdullah IH. Evaluation of human islet-specific functional quality cultured on different gas-permeable membranes. Transplant Proc 2008; 40:401-2; PMID:18374081; DOI:10.1016/j.transproceed.2008. 01.036.
21. Henquin JC, Ravier MA, Nenquin M, Jonas JC, Gilon P. Hierarchy of the beta-cell signals controlling insulin secretion. Eur J Clin Invest 2003; 33:742-50; PMID:12925032; DOI:10.1046/j.1365-2362.2003.01207.x.
22. Srivastava S, Goren HJ. Insulin constitutively secreted by beta-cells is necessary for glucose-stimulated insulin secretion. Diabetes 2003; 52:2049-56; PMID:12882922; DOI:10.2337/diabetes.52.8.2049.
23. Boffa DJ, Waka J, Thomas D, Suh S, Curran K, Sharma VK, et al. Measurement of apoptosis of intact human islets by confocal optical sectioning and stereologic analysis of YO-PRO-1-stained islets. Transplantation 2005; 79:842-5; PMID:15818328; DOI:10.1097/01.TP.0000155175.24802.73.
24. Brissova M, Fowler MJ, Nicholson WE, Chu A, Hirshberg B, Harlan DM, et al. Assessment of human pancreatic islet architecture and composition by laser scanning confocal microscopy. J Histochem Cytochem 2005; 53:1087-97; PMID:15923354; DOI:10.1369/jhc.5C6684.2005.
25. Fraker C, Timmins MR, Guarino RD, Haaland PD, Ichii H, Molano D, et al. The use of the BD oxygen biosensor system to assess isolated human islets of langerhans: oxygen consumption as a potential measure of islet potency. Cell Transplant 2006; 15:745-58; PMID:17269445; DOI:10.3727/000000006783981440.
26. Ichii H, Inverardi L, Pileggi A, Molano RD, Cabrera O, Caicedo A, et al. A novel method for the assessment of cellular composition and beta-cell viability in human islet preparations. Am J Transplant 2005; 5:1635-45; PMID:15943621; DOI:10.1111/j.1600-6143.2005.00913.x.
27. Papas KK, Colton CK, Nelson RA, Rozak PR, Avgoustiniatos ES, Scott WE, 3rd, et al. Human islet oxygen consumption rate and DNA measurements predict diabetes reversal in nude mice. Am J Transplant 2007; 7:707-13; PMID:17229069; DOI:10.1111/j.1600-6143.2006.01655.x.
28. Vandewalle B, Douillard C, Kerr Conte J, Gmyr V, Riachi R, D'Herbomez M, et al. Human pancreatic islet quality control: easy assessment of metabolic functions. Exp Clin Endocrinol Diabetes 1999; 107:214-9; PMID:10376449; DOI:10.1055/s-0029-1212101.
29. Caiazzo R, Gmyr V, Kremer B, Hubert T, Soudan B, Lukowiak B, et al. Quantitative in vivo islet potency assay in normoglycemic nude mice correlates with primary graft function after clinical transplantation. Transplantation 2008; 86:360-3; PMID:18645503; DOI:10.1097/TP.0b013e31817ef846.
30. Sabek OM, Fraga DW, Minoru O, McClaren JL, Gaber AO. Assessment of human islet viability using various mouse models. Transplant Proc 2005; 37:3415-6; PMID:16298612; DOI:10.1016/j.transproceed.2005.09.049.
31. Weber DJ. FDA regulation of allogeneic islets as a biological product. Cell Biochem Biophys 2004; 40:19-22; PMID:15289639; DOI:10.1385/CBB:40:3S:019.
32. Loganathan G, Dawra RK, Pugazhenthi S, Wiseman AC, Sanders MA, Saluja AK, et al. Culture of impure human islet fractions in the presence of alpha-1 antitrypsin prevents insulin cleavage and improves islet recovery. Transplant Proc 2010; 42:2055-7; PMID:20692406; DOI:10.1016/j.transproceed.2010.05.119.
33. Eizirik DL, Korbutt GS, Hellerstrom C. Prolonged exposure of human pancreatic islets to high glucose concentrations in vitro impairs the beta-cell function. J Clin Invest 1992; 90:1263-8; PMID:1401063; DOI:10.1172/JCI115989.
34. Cabrera O, Berman DM, Kenyon NS, Ricordi C, Berggren PO, Caicedo A. The unique cytoarchitecture of human pancreatic islets has implications for islet cell function. Proc Natl Acad Sci USA 2006; 103:2334-9; PMID:16461897; DOI:10.1073/pnas.0510790103.
35. De Marinis YZ, Zhang E, Amisten S, Taneera J, Renstrom E, Rorsman P, et al. Enhancement of glucagon secretion in mouse and human pancreatic alpha cells by protein kinase C (PKC) involves intracellular trafficking of PKCalpha and PKCdelta. Diabetologia 2010; 53:717-29; PMID:20020096; DOI:10.1007/s00125-009- 1635-x.
36. Ricordi C, Fraker C, Szust J, Al-Abdullah I, Poggioli R, Kirlew T, et al. Improved human islet isolation outcome from marginal donors following addition of oxygenated perfluorocarbon to the cold-storage solution. Transplantation 2003; 75:1524-7; PMID:12792508; DOI:10.1097/01.TP.0000058813.95063.7A.
37. Latif ZA, Noel J, Alejandro R. A simple method of staining fresh and cultured islets. Transplantation 1988; 45:827-30; PMID:2451869; DOI:10.1097/00007890-198804000-00038.
38. Ricordi C, Gray DW, Hering BJ, Kaufman DB, Warnock GL, Kneteman NM, et al. Islet isolation assessment in man and large animals. Acta Diabetol Lat 1990; 27:185-95; PMID:2075782; DOI:10.1007/BF02581331.
39. Gray DW, Morris PJ. The use of fluorescein diacetate and ethidium bromide as a viability stain for isolated islets of Langerhans. Stain Technol 1987; 62:373-81; PMID:2448922.