Culture of impure human islet fractions in the presence of alpha-1 antitrypsin prevents insulin cleavage and improves islet recovery

Transplantation Proceedings

Volumn 42, Issue 6, 2010, Pages 2055-2057

  Loganathan, G ^a   Dawra, R K ^a   Pugazhenthi, S ^b   Wiseman, A C ^b   Sanders, M A ^c   Saluja, A K ^a   Sutherland, D E R ^a   Hering, B J ^a   Balamurugan, A N ^a  

^a UNIVERSITY OF MINNESOTA MEDICAL SCHOOL   (United States)

^b UNIVERSITY OF COLORADO SCHOOL OF MEDICINE   (United States)

^c UNIVERSITY OF MINNESOTA   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALPHA 1 ANTITRYPSIN; CHYMOTRYPSIN; DODECYL SULFATE SODIUM; ELASTASE; INSULIN; PROTEINASE; TRYPSIN;

ACINAR CELL; AUTOGRAFT; CELL CULTURE; CELL VIABILITY; CONFERENCE PAPER; CONTROLLED STUDY; ELECTRON MICROSCOPY; ENZYME ACTIVITY; ENZYME LINKED IMMUNOSORBENT ASSAY; EXOCRINE CELL; HUMAN; HUMAN CELL; HUMAN TISSUE; PANCREAS ISLET; POLYACRYLAMIDE GEL ELECTROPHORESIS; PRIORITY JOURNAL; PROTEIN CLEAVAGE; PROTEIN FUNCTION; TISSUE LEVEL; TISSUE STRUCTURE;

ALPHA 1-ANTITRYPSIN; CADAVER; CELL CULTURE TECHNIQUES; CHYMOTRYPSIN; GRAFT SURVIVAL; HUMANS; INSULIN; ISLETS OF LANGERHANS; ISLETS OF LANGERHANS TRANSPLANTATION; PANCREATIC ELASTASE; TISSUE DONORS; TRANSPLANTATION, AUTOLOGOUS; TRANSPLANTATION, HOMOLOGOUS; TRYPSIN;

EID: 77955536367     PISSN: 00411345     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.transproceed.2010.05.119     Document Type: Conference Paper

References (4)

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2. A.N. Balamurugan, A.G. Breite, and T. Anazawa Successful human islet isolation and transplantation indicating the importance of class 1 collagenase and collagen degradation activity assay Transplantation 89 2010 954
3. S. Kawabata, T. Miura, and T. Morita Highly sensitive peptide-4-methylcoumaryl-7-amide substrates for blood-clotting proteases and trypsin Eur J Biochem 172 1988 17
4. M.B. Ray, V.J. Desmet, and W. Gepts Alpha-1-antitrypsin immunoreactivity in islet cells of adult human pancreas Cell Tissue Res 185 1977 63