Expression, Characterization, and site-directed mutation of a multiple herbicide-resistant acetohydroxyacid synthase (rAHAS) from pseudomonas sp. Lm10

Current Microbiology

Volumn 63, Issue 2, 2011, Pages 145-150

  Lang, Zhi Fei ^a   Shen, Jing Shen ^a   Cai, Shu Cai ^a   Zhang, Jun Zhang ^a   He, Jian He ^a   Li, Shun Peng ^a  

^a NANJING AGRICULTURAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ACETOLACTATE SYNTHASE; FLUMETSULAM; HERBICIDE; IMAZETHAPYR; IMIDAZOLINONE; METSULFURON METHYL; PYRIMINOBAC METHYL; SULFONYLUREA; TRIAZOLOPYRIMIDINE SULFONAMIDE; UNCLASSIFIED DRUG;

ACETOHYDROXYACID SYNTHASE GENE; ARTICLE; BACTERIAL GENE; CONTROLLED STUDY; DNA SEQUENCE; ENZYME ACTIVITY; ENZYME PURIFICATION; GENE OVEREXPRESSION; HERBICIDE RESISTANCE; MOLECULAR DYNAMICS; MOLECULAR WEIGHT; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN DETERMINATION; PROTEIN EXPRESSION; PSEUDOMONAS PUTIDA; SENSITIVITY ANALYSIS; SEQUENCE ANALYSIS; SITE DIRECTED MUTAGENESIS;

ACETOLACTATE SYNTHASE; AMINO ACID SUBSTITUTION; CLONING, MOLECULAR; DNA, BACTERIAL; ENZYME INHIBITORS; GENE EXPRESSION; HERBICIDES; KINETICS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, SITE-DIRECTED; MUTANT PROTEINS; PROTEIN SUBUNITS; PSEUDOMONAS; RECOMBINANT PROTEINS; SEQUENCE ANALYSIS, DNA;

PSEUDOMONAS PUTIDA; PSEUDOMONAS SP.;

EID: 80052517768     PISSN: 03438651     EISSN: 14320991     Source Type: Journal    
DOI: 10.1007/s00284-011-9953-x     Document Type: Article

References (27)

1. Bernasconi P, Woodworth AR, Rosen BA, Subramanian MV, Siehl DL (1995) A naturally occurring point mutation confers broad range tolerance to herbicides that target acetolactate synthase. J Biol Chem 270:17381-17385
2. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-254
3. Chang AK, Duggleby RG (1998) Herbicide-resistant forms of Arabidopsis thaliana acetohydroxyacid synthase: characterization of the catalytic properties and sensitivity to inhibitors of four defined mutants. Biochem J 333:765-777 (Pubitemid 28398547)
4. Duggleby RG, Pang SS (2000) Acetohydroxyacid synthase. J Biochem Mol Biol 33:1-36
5. Duggleby RG, Pang SS, Yu HQ, Guddat LW (2003) Systematic characterization of mutations in yeast acetohydroxyacid synthase. Eur J Biochem 270:2895-2904
6. Falco SC, McDevitt RE, Chui CF, Hartnett ME, Knowlton S, Mauvais CJ, Smith JK, Mazur BJ (1989) Engineering herbicideresistant acetolactate synthase. Dev Ind Microbiol 30:187-194
7. Harms CT, Armour SL, DiMaio JJ, Middlesteadt LA, Murray D, Negrotto DV, Thompson TH, Weymann K, Montoya AL, Shillito RD, Jen GC (1992) Herbicide resistance due to amplification of a mutant acetohydroxyacid synthase gene. Mol Gen Genet 233:427-435
8. Hattori J, Rutledge R, Labbé H, Brown D, Sunohara G, Miki B (1992) Multiple resistance to sulfonylureas and imidazolinones conferred by an acetohydroxyacid synthase gene with separate mutations for selective resistance. Mol Gen Genet 232:167-173
9. Hattori J, Brown D, Mourad G, Labbé H, Ouellet T, Sunohara G, Rutledge R, King J, Miki B (1995) An acetohydroxy acid synthase mutant reveals a single site involved in multiple herbicide resistance. Mol Gen Genet 246:419-425
10. Haughn GW, Smith J, Mazur B, Somerville C (1988) Transformation with a mutant Arabidopsis acetolactate synthase gene renders tobacco resistant to sulfonylurea herbicides. Mol Gen Genet 211:266-271
11. Hill CM, Duggleby RG (1998) Mutagenesis of E. coli acetohydroxyacid synthase isozyme II and characterization of three herbicide-resistant forms. Biochem J 335:653-661 (Pubitemid 28515421)
12. Huang X, He J, Sun XF, Sun JQ, Li YF, Shen JJ, Li SP (2010) Characterization and molecular mechanism of a naturally occurring metsulfuron-methyl resistant strain of Pseudomonas aeruginosa. World J Microbiol Biotechnol 26:515-521
13. Ibdah M, Bar-Ilan A, Livnah O, Schloss JV, Barak Z, Chipman DM (1996) Homology modeling of the structure of bacterial acetohydroxy acid synthase and examination of the active site by site-directed mutagenesis. Biochemistry 35:16282-16291 (Pubitemid 27044090)
14. Lee KY, Townsend J, Tepperman J, Black M, Chui CF, Mazur B, Dunsmuir P, Bedbrook J (1988) The molecular basis of sulfonylurea herbicide resistance in tobacco. EMBO J 7:1241-1248
15. Lee YT, Chang AK, Duggleby RG (1999) Effect of mutagenesis at serine 653 of Arabidopsis thaliana acetohydroxyacid synthase on the sensitivity to imidazolinone and sulfonylurea herbicides. FEBS Lett 452:341-345 (Pubitemid 29301772)
16. Mourad G, King J (1992) Effect of four classes of herbicides on growth and acetolactate-synthase activity in several variants of Arabidopsis thaliana. Planta 188:491-497
17. Pang SS, Duggleby RG, Guddat LW (2002) Crystal structure of yeast acetohydroxyacid synthase: a target for herbicidal inhibitors. J Mol Biol 317:249-262 (Pubitemid 34722192)
18. Pang SS, Guddat LW, Duggleby RG (2003) Molecular basis of sulfonylurea herbicide inhibition of acetohydroxyacid synthase. J Biol Chem 278:7639-7644 (Pubitemid 36800773)
19. Rajasekaran K, Grula JW, Anderson DM (1996) Selection and characterization of mutant cotton (Gossypium hirsutum L.) cell lines resistant to sulfonylurea and imidazolinone herbicides. Plant Sci 119:115-124 (Pubitemid 27076818)
20. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY
21. Sathasvian K, Haughn GW, Murai N (1991) Molecular basis of imidazolinone herbicide resistance in Arabidopsis thaliana var columbia. Plant Physiol 97:1044-1050 (Pubitemid 21914326)
22. Sun XF, Huang X, Chen B, Li SP, He J (2008) Isolation of a metsulfuron-methyl-resistant bacteria and cloning and expression of the acetohydroxyacid synthase genes ilvIH. Acta Microbiologica Sinica 48:149-1493
23. Wang BZ, Guo P, Hang BJ, Li L, He J, Li SP (2009) Cloning of a novel pyrethroid-hydrolyzing carboxylesterase gene from sphingobium sp. strain JZ-1 and characterization of the gene product. Appl Environ Microbiol 75:5496-5500
24. Wang GL, Li R, Li SP, Jiang JD (2010) A novel hydrolytic dehalogenase for the chlorinated aromatic compound chlorothalonil. J Bacteriol 192:2737-2745
25. Xie Q, Jiménez A (1996) Molecular cloning of a novel allele of SMR1 which determines sulfometuron methyl resistance in Saccharomyces cerevisiae. FEMS Microbiol Lett 137:165-168 (Pubitemid 26176543)
26. Yadav N, McDevitt RE, Benard S, Falco SC (1986) Single amino acid substitutions in the enzyme acetolactate synthase confer resistance to the herbicide sulfometuron methyl. Proc Natl Acad Sci 83:4418-4422 (Pubitemid 16057059)
27. Yoon TY, Chung SM, Chang SI, Yoon MY, Hahn TR, Choia JD (2002) Roles of lysine 219 and 255 residues in tobacco acetolactate synthase. Biochem Biophys Res Commun 293:433-439