Characterization of a novel immobilized biocatalyst obtained by matrix-assisted refolding of recombinant polyhydroxyoctanoate depolymerase from Pseudomonas putida KT2442 isolated from inclusion bodies

Journal of Industrial Microbiology and Biotechnology

Volumn 38, Issue 9, 2011, Pages 1203-1209

  Arroyo, M ^a   Garcia Hidalgo J ^a,b   Villalon M ^a   De Eugenio, L ^b   Hormigo, D ^b   Acebal, C ^a   Garcia J L ^b   Prieto, M A ^b   De La Mata, Isabel ^a  

^a UNIVERSIDAD COMPLUTENSE DE MADRID   (Spain)

^b CENTRO DE INVESTIGACIONES BIOLÓGICAS   (Spain)

Author keywords

Immobilization; PHA depolymerase; Polyhydroxyalkanoate; Refolding

Indexed keywords

4 NITROPHENYL ALKANOATE ESTER; AGAROSE; ESTER DERIVATIVE; NITRILOTRIACETIC ACID; PHOSPHATE; POLYHISTIDINE TAG; RECOMBINANT POLYHYDROXYOCTANOATE DEPOLYMERASE; UNCLASSIFIED DRUG; UREA;

ARTICLE; BACTERIUM ISOLATION; BIOCATALYST; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME IMMOBILIZATION; ENZYME PURIFICATION; ESCHERICHIA COLI; NONHUMAN; PH; PROTEIN HYDROLYSIS; PROTEIN REFOLDING; PSEUDOMONAS PUTIDA;

BIOCATALYSIS; CARBOXYLIC ESTER HYDROLASES; ENZYMES, IMMOBILIZED; ESCHERICHIA COLI; HYDROLYSIS; INCLUSION BODIES; PROTEIN REFOLDING; PSEUDOMONAS PUTIDA; RECOMBINANT PROTEINS;

PSEUDOMONAS PUTIDA;

EID: 80052508281     PISSN: 13675435     EISSN: 14765535     Source Type: Journal    
DOI: 10.1007/s10295-010-0898-z     Document Type: Article

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